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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,245 results for: grna

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Showing: 2061 - 2080 of 16245 results

  3. pJB07

    Plasmid
    #190481
    Purpose
    Targeting plasmid for 2-plasmid C. difficile mutagenesis system
    Depositor
    Joseph Sorg
    Publication
    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Upstream & Downstream pyrE deletion region
    gRNA
    Use
    E. coli / b. subtilis - c. difficile shuttle vect…
    Available since
    Sept. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    190481-plasmid-map-sequence-id-372027

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  4. pEF005-sgRNA-shuffle-in

    Plasmid
    #104441
    Purpose
    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.
    Depositor
    Detlef Weigel
    Publication
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Insert
    sgRNA-transRNA transcription by U6 promoter
    Use
    CRISPR
    Expression
    Bacterial and Plant
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104441-plasmid-map-sequence-id-201145

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  5. pEF004-sgRNA-shuffle-in

    Plasmid
    #104440
    Purpose
    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005
    Depositor
    Detlef Weigel
    Publication
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Insert
    sgRNA-transRNA transcription by U6 promoter
    Use
    CRISPR
    Expression
    Bacterial and Plant
    Promoter
    U6 promoter
    Available since
    Dec. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    104440-plasmid-map-sequence-id-201143

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  6. pMOD_B2312

    Plasmid
    #91070
    Purpose
    Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    Use
    CRISPR
    Promoter
    PvUbi1
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91070-plasmid-map-sequence-id-181878

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  7. pMOD_B2309

    Plasmid
    #91069
    Purpose
    Module B, Promoter: YAO, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    Use
    CRISPR
    Promoter
    YAO
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91069-plasmid-map-sequence-id-181875

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  8. pMOD_B2101

    Plasmid
    #91060
    Purpose
    Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Promoter
    35S
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91060-plasmid-map-sequence-id-181852

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  9. pDIRECT_26G

    Plasmid
    #91149
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91149-plasmid-map-sequence-id-182283

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  10. pDIRECT_21B

    Plasmid
    #91129
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91129-plasmid-map-sequence-id-182252

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  15. CIRTS-YTHDF2-Malat1

    Plasmid
    #216858
    Purpose
    CIRTS RNA targeting system for targeted knockdown of m6A-containing Malat1 at the synapse. CIRTS-YTHDF2-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Publication
    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100
    Insert
    CIRTS-YTHDF2-Calm3-U6-Malat1 gRNA
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    Syn1, U6
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216858-plasmid-map-sequence-id-443348

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  16. CIRTS-FTO-Malat1

    Plasmid
    #216860
    Purpose
    CIRTS RNA targeting system for targeted removal of m6A on Malat1 at the synapse. CIRTS-FTO-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Publication
    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100
    Insert
    CIRTS-FTO-Calm3-U6-Malat1 gRNA
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Syn1, U6
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    216860-plasmid-map-sequence-id-434854

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  19. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

    Plasmid
    #133794
    Purpose
    U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Publication
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    NmeCas9 and Nme2Cas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Amrani et al. Genome Biol…
    Promoter
    U6
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133794-plasmid-map-sequence-id-268076

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Showing: 2061 - 2080 of 16245 results