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pBAD-HisD-BR1

Plasmid

#192816

Purpose

Expresses BR1 in bacteria

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

BR1 (BRI1 Mustard Weed)

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-Y530EEI

Plasmid

#140322

Purpose

Expresses HA-tagged human Src-Y530EEI in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

Y530EEI

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-ORF30-2xStrep

Plasmid

#130958

Purpose

Lentiviral vector for expression of KSHV ORF30 with a C-terminal 2xStrep tag

Depositor

Britt Glaunsinger

Article

Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

Insert

ORF30 (ORF30 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))

Use

Lentiviral

Tags

2xStrep

Promoter

CMV

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-ORF24-3xFLAG

Plasmid

#130959

Purpose

Lentiviral vector for expression of KSHV ORF24 with a C-terminal 3xFLAG tag

Depositor

Britt Glaunsinger

Article

Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

Insert

ORF24 (ORF24 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))

Use

Lentiviral

Tags

3xFLAG

Promoter

CMV

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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mei-S332 5.6Kb genomic DNA in Casper4

Plasmid

#112984

Purpose

P element vector for transposon insertion of mei-S332 5.6Kb genomic DNA into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

Insert

mei-S332 (Sgo) (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pSC45

Plasmid

#104819

Purpose

CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-2). Also expresses Cas9 from Gmubi promoter.

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Insert

Glyma.04g057400

Use

CRISPR

Expression

Plant

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pVRa17_1458

Plasmid

#49671

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF17_1458

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa14_1324

Plasmid

#49665

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF14_1324

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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psicheck2 PTEN 3'UTR

Plasmid

#50936

Purpose

reporter for PTEN RNAi

Depositor

Pier Pandolfi

Article

Tay et al Cell. 2011 Oct 14;147(2):344-57. doi: 10

Insert

PTEN 3'UTR (PTEN Human)

Use

RNAi

Tags

Renilla luciferase

Expression

Mammalian

Promoter

T7

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MTS-WT-c-Src-FLAG

Plasmid

#44652

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (res…

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO-3XFLAG Halo WT nsp1

Plasmid

#175412

Purpose

Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 nsp1 (ORF1ab CoV2)

Tags

3XFLAG Halo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MTS-CA-c-Src-FLAG

Plasmid

#44654

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed Tyr 530 to Phe

Promoter

CMV promoter

Available Since

May 6, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MTS-KD-c-Src-FLAG

Plasmid

#44653

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed lysine 298 to methionine

Promoter

CMV promoter

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

Plasmid

#175421

Purpose

Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR E2 HSPter-RPS5Ap-mito

Plasmid

#163146

Purpose

Plant mitoTALEN vector assembly, Entry clone 2 with attL1 L4; Multisite Gateway entry vector to terminate the E1 ORF and having RPS5A promoter with mitochondrial presequence for the E3 ORF

Depositor

Shin-ichi Arimura

Article

Arimura et al Plant J. 2020 Oct 24. doi: 10.1111/tpj.1

Insert

A terminator of Arabidopsis heat shock protein, RPS5A promoter, and mitochondrial presequence

Use

Entry vector

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO-3XFLAG Halo delta 1-117 nsp1

Plasmid

#175423

Purpose

Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 delta 1-117 nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

nsp1 C-terminus

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo delta 122-130 nsp1

Plasmid

#175425

Purpose

Expresses delta 122-130 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 delta 122-130 nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo delta 118-180 nsp1

Plasmid

#175424

Purpose

Expresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 delta 118-180 nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

N-terminus of nsp1

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO-3XFLAG Halo R124A/K125A nsp1

Plasmid

#175417

Purpose

Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 R124A/K125A nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

R124A and K125A

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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bactin:mKate2-ras

Plasmid

#105963

Purpose

transgenesis, ubiquitous expression

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

Ras (KRAS Human)

Tags

mKate2

Expression

Bacterial

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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