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Flag-Vac 14 Full Length

Plasmid

#47418

Depositor

Peter McPherson

Article

Lemaire et al FEBS Lett. 2006 Dec 22;580(30):6948-54.

Insert

Vac14 (VAC14 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pSTARR-seq_fly

Plasmid

#71499

Purpose

flySTARR-seq screening vector with DSCP core promoter.

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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shHIF2α(#2) (HIF2α-#3-pSUPER-retro-GFP/Neo)

Plasmid

#22132

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-GFP-EWSR1-myc-His

Plasmid

#46385

Purpose

expresses EGFP tagged EWSR1 in mammalian cells

Depositor

Heinz Gehring

Article

Pahlich et al Proteins. 2008 Sep;72(4):1125-37. doi: 1

Insert

EWSR1 (EWSR1 Human)

Tags

EGFP, His, and myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
p5'Entry-lck-5.5kb

Plasmid

#58890

Purpose

provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes

Depositor

Jeffrey Yoder

Insert

zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb

Use

Gateway 5' entry clone

Mutation

mutated single nucleotide (T-->C, 13th nucleot…

Promoter

lymphocyte-specific protein tyrosine kinase (lck)…

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2 (S939A)

Plasmid

#14132

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 S939A (TSC2 Human)

Tags

Flag

Expression

Mammalian

Mutation

S939A.

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mBeRFP

Plasmid

#175173

Purpose

large-stokes shift red fluorescent protein mBeRFP

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

mBeRFP

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_NOTCH2_WT

Plasmid

#82292

Purpose

Gateway Donor vector containing NOTCH2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NOTCH2 (NOTCH2 Human)

Use

Gateway Cloning

Mutation

C946Y

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)

Plasmid

#110559

Purpose

miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-BRD1

Plasmid

#65375

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD1 (BRD1 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-DHFR

Plasmid

#80329

Purpose

Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

DHFR-TSc3

Use

CRISPR

Promoter

DHFR-TS 5' UTR

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEBTet_NADP-Snifit-mito

Plasmid

#113919

Purpose

Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mTagBFP2

Plasmid

#96935

Purpose

Retroviral plasmid containing mTagBFP2 for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Tag Blue Fluorescent Protein

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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β₂AR-mCFP

Plasmid

#38260

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

β₂AR (ADRB2 Human)

Tags

Flag, mCFP, and signal peptide sequence (MKTIIALS…

Expression

Mammalian

Mutation

diglycine linker (replacing terminator codon)

Promoter

CMV

Available Since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
560HTR

Plasmid

#24444

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis

Expression

Bacterial

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
pTn Donor

Plasmid

#236211

Purpose

Arabinose-inducible Tn5 InducTn-seq plasmid

Depositor

Matthew Waldor

Article

Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

Insert

None

Expression

Bacterial

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pgLAP5

Plasmid

#19706

Depositor

Peter Jackson

Insert

pgLAP5

Tags

EGFP, Precission Protease site, and S peptide

Expression

Mammalian

Available Since

Feb. 19, 2009

Availability

Academic Institutions and Nonprofits only

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pJH-MBP-CbAgo

Plasmid

#127707

Purpose

pET His6 MBP TEV CbAgo

Depositor

John van der Oost

Article

Hegge et al Nucleic Acids Res. 2019 May 9. pii: 5487

Insert

CbAgo

Tags

MPB

Expression

Bacterial

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMN437

Plasmid

#32362

Depositor

Michael Niederweis

Article

Song et al Tuberculosis (Edinb). 2008 Nov;88(6):526

Insert

gfpm2+

Use

Mycobacterial gfp expression vector

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pPAICS-EGFP

Plasmid

#99108

Purpose

Expresses human PAICS-EGFP fusion in mammalian cells

Depositor

Stephen Benkovic

Article

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

PAICS (PAICS Human)

Tags

EGFP (enhanced green fluorescent protein)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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