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Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

Plasmid

#138460

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

MCP-KRAB-IRES-zsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK2

Plasmid

#122673

Purpose

Expresses mouse PCSK2 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2) (Pcsk2 Mouse)

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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EcoHIV-mScarlet

Plasmid

#235598

Purpose

EcoHIV-mScarlet is a modification of pNL4-3-EcoHIV (Potash et al, PNAS 2005). EcoHIV is rodent-infectable modification of HIV where HIV env replaced for MLV-env

Depositor

Michael Shtutman

Article

Li et al J Vis Exp. 2024 Dec 20;(214). doi: 10.37

Insert

NL4-3 EcoHIV mScarlet

Use

Lentiviral

Tags

mScarlet

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ40-Flag-Ferritin-iBox-PAK4cat

Plasmid

#190180

Purpose

expresses Flag-tagged Ferritin-iBox-PAK4cat

Depositor

Bianxiao Cui

Article

Li et al Nano Lett. 2019 Oct 9;19(10):6955-6963.

Insert

Ferritin-iBox-PAK4cat

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PCMV-flag-Caspase11

Plasmid

#21145

Depositor

Junying Yuan

Article

Li et al Nat Cell Biol. 2007 Mar . 9(3):276-86.

Insert

Caspase11 (Casp4 Mouse)

Tags

flag

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hSAPS1-HA

Plasmid

#165466

Purpose

Human SAPS1 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Protein phosphatase 6 regulatory subunit 1 (PPP6R1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSBtet-RH-β+ε

Plasmid

#247178

Purpose

For generating Sleeping Beauty-based stable cell line expressing human muscle acetylcholine receptor beta1 and epsilon subunits. Expresses RFP; hygromycin selection.

Depositor

Ryan Hibbs

Article

Li et al Cell. 2025 May 1;188(9):2390-2406.e20. d

Insert

Human muscle acetylcholine receptor beta and epsilon subunits

Tags

3xFLAG tag

Expression

Mammalian

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSBtet-GP-α+δ

Plasmid

#247177

Purpose

For generating Sleeping Beauty-based stable cell line expressing human muscle acetylcholine receptor alpha1 and delta subunits. Expresses GFP; puromycin selection.

Depositor

Ryan Hibbs

Article

Li et al Cell. 2025 May 1;188(9):2390-2406.e20. d

Insert

Human muscle acetylcholine receptor alpha1 and delta subunits

Tags

Twin strep tag

Expression

Mammalian

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-th-sgRNA

Plasmid

#65565

Purpose

in vitro trancription of sgRNA targeting the zebrafish th locus

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

th sgRNA (th Zebrafish)

Use

In vitro rna transcription

Promoter

T7

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pUC57-GFP-SFPQ-RT

Plasmid

#97090

Purpose

Encodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

EGFP-SFPQ-RT (SFPQ Human)

Use

CRISPR

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform II-EGFP

Plasmid

#120316

Purpose

Septin 11 isoform II mammalian expression (EGFP-tag at the C-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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Luc-pSUPER-Retro-GFP/Neo

Plasmid

#22133

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Available Since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pAcGHLT-B-DDB1

Plasmid

#48638

Purpose

Expresses Full Lenght DDB1 in Insect Cells (ampR)

Depositor

Ning Zheng

Article

Li et al Cell. 2006 Jan 13;124(1):105-17.

Insert

DDB1 (DDB1 Human)

Expression

Insect

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mPCSK7

Plasmid

#122676

Purpose

Expresses mouse PCSK7 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 7 (Pcsk7), transcript variant 2 (Pcsk7 Mouse)

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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FHpHG_mTent5a

Plasmid

#221439

Purpose

Transfection into the cells for the reporter or qPCR assay.

Depositor

Marcos Morgan

Article

Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

Insert

Tent5a (Fam46a Mouse)

Tags

FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

Expression

Mammalian

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a(+)-PE2

Plasmid

#180015

Purpose

Expressing PE2 protein in E.coli for protein purification

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

PE2

Tags

6xHis

Expression

Bacterial

Promoter

Lac operator-controlled T7 promoter

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Lsg-GBA-1 + 2

Plasmid

#199276

Purpose

nicking sgRNA to induce or correct GBA (c. 1226 A > G) mutation using PE3

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

GBA nick

Expression

Mammalian

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRIP3-mCherry

Plasmid

#61386

Purpose

Expresses RIP3-mCherry in mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

RIP3 (RIPK3 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mPCSK4

Plasmid

#122675

Purpose

Expresses mouse PCSK4 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 4 (Pcsk4) (Pcsk4 Mouse)

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pET11a-CA(A14C, E45C)

Plasmid

#79045

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

CA (A14C, E45C)

Expression

Bacterial

Mutation

A14C and E45C mutations in HIV-1 CA protein

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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