We narrowed to 7,041 results for: Ank
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Plasmid#113820PurposeCRISPR donor plasmid to tag human transcription factor ISL1 with GFPDepositorInsertISL1 homology arms flanking EGFP-IRES-Neo cassette (ISL1 Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB2375
Plasmid#67546PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae XI-1 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTalpha1-EGFP-N1
Plasmid#133929PurposeNeuron-specific expression of EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationEGFPPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOX + kqt3
Plasmid#16138DepositorInsertCaenorhabditis elegans potassium channel (kqt-3 Nematode)
UseXenopus oocyte expressionTagsExpressionMutationlacks aa1-16 compared to GenBank reference AY5729…PromoterAvailable sinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pE-01
Plasmid#166930PurposeStop codon part (E)DepositorInsertSTOP CODON TAA and spacer - BLOCK E- Flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD-01
Plasmid#166929PurposeTrrnB and L3S2P21 terminator part (D)DepositorInsertDOUBLE TERMINATOR ( TrrnB - L3S2P21) _BLOCK D - Flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB-01
Plasmid#166924PurposePtac promoter part (B)DepositorInsertPTAC promoter - Block B- Flanked by BsaI restriction sites.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pA-01
Plasmid#166923PurposeLacI expression cassette (A)DepositorInsertLacI expression cassette - Block A- Flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
p817-mgammaFcry-mCherry
Plasmid#23154DepositorInsertmouse gammaF crystallin promoter:mCherry (Crygf Mouse)
UseMulticloning site flanked by i-scei sitesTagsmCherryExpressionMutationmouse gammaF crystallin promoter (-225 - +73) pre…PromoterAvailable sinceApril 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCfB2224
Plasmid#67547PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKG204
Plasmid#63078PurposeRetroviral expression of EGFP-TEV-S-tag HP1 alpha for protein localization and affinity purification (LAP)DepositorInsertHP1 alpha (CBX5 Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationV151I mutation compared to GenBank reference sequ…PromoterAvailable sinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL4.75-KRAS LCS6m
Plasmid#44571DepositorInsertKRAS 3'UTR (KRAS Human)
UseTagsExpressionMammalianMutationThe T allele at LCS6 (let-7 complementary site 6)…PromoterCMVAvailable sinceApril 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNM261
Plasmid#128411PurposeVanillic acid inducible production of lysostaphin. LsyT with no signaling peptide for secretion.DepositorInsertLysostaphin (epr Staphylococcus simulans)
UseTagsExpressionBacterialMutationPromoterPspank(V)Available sinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
dg264_pENTR_ChronosCel
Plasmid#66102PurposeEntry clone containing Chronos coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2DepositorInsertChronos
UseGatewayTagsExpressionMutationPromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMLX-donor
Plasmid#112370PurposeCRISPR donor plasmid to tag human transcription factor MLX with GFPDepositorInsertMLX homology arms flanking EGFP-IRES-Neo cassette (MLX Human)
UseCRISPRTagsExpressionMutationhg38:17:42570798:G>A, hg38:17:42570950:T>G,…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHOXB13-donor
Plasmid#113814PurposeCRISPR donor plasmid to tag human transcription factor HOXB13 with GFPDepositorInsertHOXB13 homology arms flanking EGFP-IRES-Neo cassette (HOXB13 Human)
UseCRISPRTagsExpressionMutationrs139887310PromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLP-TRE2-hSox2
Plasmid#49392Purposeinducible (dox) expression of hSox2DepositorInsertSox2 (SOX2 Human)
UseCre/Lox; Donor vectorTags6xHNExpressionMammalianMutationTGA (stop) to CCA (Pro) at nt 1027 with respect t…PromoterTet-responsiveAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMST694_BB3_pTet-on_mttA_trpC_pcoxA:pyrGtrunc_AMA2.8
Plasmid#90292PurposeBB3 for mttA integration with homologous flanks at pyrG locus, hygromycin resistanceDepositorInsertmttA
UseA nigerTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRVV584
Plasmid#108280PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRVV669
Plasmid#108283PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFOXO4-donor
Plasmid#112312PurposeCRISPR donor plasmid to tag human transcription factor FOXO4 with GFPDepositorInsertFOXO4 homology arms flanking EGFP-IRES-Neo cassette (FOXO4 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRVV613
Plasmid#108281PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site
Plasmid#228633PurposeBacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)DepositorInsertEnterovirus Coxsackievirus A71 2A protease
UseTagsHis-SUMOExpressionBacterialMutationC110APromoterAvailable sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain
Plasmid#228663PurposeBacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)DepositorInsertZika virus portion of NS2B protease coexpressed with NS3 protease
UseTagsHis-GST-TEVExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease fusion with non cleavable linker (N-terminal Twin-Strep-tag)
Plasmid#228661PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Zika virus for biochemical assayDepositorInsertZika virus portion of NS2B protease fused to NS3
UseTagsHis-Twin Strep-TevExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSS1 - pBait-5GC[1xMS2]-empty
Plasmid#222405Purposearabinose-induced synthesis of an RNA containing an MS2 hairpin flanked by a 5GC clamp. Used as a three-hybrid control. For cloning purposes, see plasmid #222403.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUPD2_miniDFR-att-OCSterm-att-miniDFR (GB4641)
Plasmid#225431PurposeOCS terminator flanked by two att sites of PhiC31 integrase located at position 112 of the SlDFR minimal promoterDepositorInsertminiDFR-att-OCSterm-att-miniDFR
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLXB3o1 HygR-MAR10-gemiGFP-MAR10 (GB4684)
Plasmid#225439PurposeModule for stable transformation of Geminino 1.0-eGFP flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.DepositorInsertHygR-MAR10-gemiGFP-MAR10
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
MERS Mpro - His6-SUMO with autoprocessing - MSAVLQ/SGLVKM---MGVVMQ/SGVRKVTYGTAHWL
Plasmid#204814PurposeBacterial expression of MERS MproDepositorInsertMERS Mpro (orf1ab MERS)
UseTagsExpressionBacterialMutationMSAVLQ/SGLVKMPromoterAvailable sinceFeb. 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHAP8
Plasmid#206857PurposeA plasmid containing a 11,944 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP7
Plasmid#206853PurposeA plasmid containing a 13,748 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP6
Plasmid#206852PurposeA plasmid containing a 15,671 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP5
Plasmid#206851PurposeA plasmid containing a 17,639 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP3_URA
Plasmid#206848PurposeA plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was integrated into the chloroplast fragment, which is flanked by SapI sites.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK1061
Plasmid#209550PurposeBTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome in place of the clpA geneDepositorInsertE2-Crimson cassette flanked with homology arms for recombination into the S. alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 2A protease; aliases: Enterovirus EV71 2A protease, 6xHis-SUMO-TEV tag, self cleaving
Plasmid#204810PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBetaActin-dCer3-C2-dCer3
Plasmid#191328PurposeExpresses the fluorescently labeled membrane anchor C2 without the chemical dimerization domain FRB as a control for recruiting proteins to plasma membraneDepositorInsertdCer3-C2-dCer3
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
50_p5E_UBI_loxTagBFP
Plasmid#172518Purposep5E tol2kit compatible clone encoding a Ubiquitin promoter and a tagBFP sequence terminated by 3x polyA sequences and flanked by floxP sites for CRE-excisionDepositorInsertubiquitin promoter, TagBFP, loxP sites (inversion)
UseTagsExpressionMutationPromoterUbiquitinAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-mScarlet
Plasmid#191324PurposeExpresses fluorescent labeled beta Tubulin to measure microtubule densityDepositorInsertTUBB2a-mScarlet (Tubb2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMLS713
Plasmid#188885PurposeMultisite Gateway [4-3] Destination vector; miniMos transposon with lox2272-flanked Cbr-unc-119 markerDepositorTypeEmpty backboneUseMultisite gateway [4-3] destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMLS715
Plasmid#188887PurposeMultisite Gateway [4-3] Destination vector; lox2272-flanked Cbr-unc-119 marker; homology to 5' miniMos ArmDepositorTypeEmpty backboneUseMultisite gateway [4-3] destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
PROM5_Ntprrn_rev_chlo
Plasmid#163952PurposeL0 part - promoterDepositorInsertfrom GenBank: KT364744.3
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PROM5_Ntpsba_chlo
Plasmid#163953PurposeL0 part - promoterDepositorInsertfrom GenBank: KT364744.3
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pG-02
Plasmid#166933PurposeTarget AD1.A5 part (G)DepositorInsertStar sense AD1.S5 +RBS - Block G- Flanked by BsaI restriction sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC-03
Plasmid#166927PurposeSTAR-AD1.A6 part (C)DepositorInsertStar Antisense AD1.A6 - Block C - Flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC-02
Plasmid#166926PurposeSTAR-AD1.A5 part (C)DepositorInsertStar Antisense AD1.A5- Block C- Flanked by BsaI restriction sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM0619
Plasmid#128994PurposeSpacer cassette plasmid flanked by ConL2 and ConREDepositorInsertConL2-Spacer-ConRE-AmpR-Col-E1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHOXC13-donor
Plasmid#113827PurposeCRISPR donor plasmid to tag human transcription factor HOXC13 with GFPDepositorInsertHOXC13 homology arms flanking EGFP-IRES-Neo cassette (HOXC13 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGLIS3-donor
Plasmid#113812PurposeCRISPR donor plasmid to tag human transcription factor GLIS3 with GFPDepositorInsertGLIS3 homology arms flanking EGFP-IRES-Neo cassette (GLIS3 Human)
UseCRISPRTagsExpressionMutationrs10118031, rs10973702, rs912257PromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSRF-donor
Plasmid#112362PurposeCRISPR donor plasmid to tag human transcription factor SRF with GFPDepositorInsertSRF homology arms flanking EGFP-IRES-Neo cassette (SRF Human)
UseCRISPRTagsExpressionMutationhg38:6:43178470:C>A, hg38:6:43178514:G>APromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only