We narrowed to 4,215 results for: bre

1. cyclin D1-HA-pBabe-Hygro

   Plasmid

   #22703

   Depositor

   William Kaelin

   Article

   Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

   Insert

   cyclin D1 (CCND1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt cDNA

   Promoter

   Available since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICE-HA-MRE11-H63S

   Plasmid

   #82035

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of H63S mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Patrick Calsou, Sébastien Britton

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   MRE11 (MRE11 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H63S MRE11 mutant

   Promoter

   CMV-tet

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-shLacZ-1735

   Plasmid

   #115454

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   shLacZ-1735
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry Codon 59 sgRNA

   Plasmid

   #109431

   Purpose

   MLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporter

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   mCherry codon 59 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. myc-hRAD18

   Plasmid

   #68827

   Purpose

   Expresses c-myc-tagged human RAD18

   Depositor

   Satoshi Tateishi

   Article

   Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

   Insert

   c-myc tagged human RAD18 (RAD18 Human)
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pSL0812 (pCRISPR(BbsI)_Vch)

   Plasmid

   #200898

   Purpose

   Expresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCRISPR (entry)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-hCas9-T2A-mStrawberry

   Plasmid

   #187224

   Purpose

   Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Inserted T2A-mStrawberry at 3' terminal of M…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas9-NLS-gs10-cTRb413

   Plasmid

   #188261

   Purpose

   Plasmid ensures constitutive expression of the C-terminal fragment of split TRbeta (aa 413-460), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   cTRb413 (TXNRD2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. eGFP-SUN1

   Plasmid

   #213515

   Purpose

   Expresses human eGFP-SUN1 in mammalian cells

   Depositor

   Karim Mekhail

   Article

   Shokrollahi et al bioRxiv 2023.05.07.539750

   Insert

   SUN1 (SUN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SUN1 has a silent mutation on its gene (nucleotid…

   Promoter

   CMV

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hNR2F2-RFP

    Plasmid

    #22926

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 . 3():19.

    Insert

    human nuclear receptor subfamily 2 group F member 2 promoter driving DsRedExpress (NR2F2 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIRES_EGFP_GA_KCNE1

    Plasmid

    #173160

    Purpose

    For mammalian expression of KCNE1 and creating variants

    Depositor

    Al George

    Article

    Kuenze et al Elife. 2020 Oct 23;9. pii: 57680. doi: 1

    Insert

    KCNE1 (KCNE1 Human)
    

    Use

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites)

    Plasmid

    #120297

    Purpose

    AAV vector for expression of AcrIIA4 (no miR binding sites, control vector)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIA4
    

    Use

    AAV and CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-AC3CtermGenomic-mCherry

    Plasmid

    #105250

    Purpose

    mouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    mCherry (with STOP and extra-sequence) with homology arms for mouse Adcy3 (Adcy3 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDest-636

    Plasmid

    #159574

    Purpose

    Gateway Destination vector for protein expression in baculovirus

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Type

    Empty backbone

    Use

    Tags

    His6-MBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVimentin-miRFP703

    Plasmid

    #79996

    Purpose

    Vimentin labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    miRFP703

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETDuet-1 TIM8,13

    Plasmid

    #170282

    Purpose

    Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea

    Insert

    yeast Tim8 and Tim13 (TIM8 Budding Yeast)
    

    Use

    Tags

    TEV-cleavable N-terminal His tag on Tim13 (on bac…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF1a-2xSV40_NLS-NLuc

    Plasmid

    #135953

    Purpose

    Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Use

    Tags

    2xSV40-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLdCH

    Plasmid

    #84291

    Purpose

    Express gRNA and Cas9 in Leishmania with Hygromycin resistance

    Depositor

    Greg Matlashewski

    Article

    Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

    Insert

    gRNA and Cas9
    

    Use

    CRISPR; Leishmania

    Tags

    Expression

    Mutation

    Promoter

    L. donovani ribosome RNA promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. LightR-iCre-miRFP670

    Plasmid

    #162158

    Purpose

    Expresses LightR-iCre-miRFP670 in mammalian cells

    Depositor

    Andrei Karginov

    Article

    Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

    Insert

    LightR-iCre-miRFP670
    

    Use

    Tags

    NLS and miRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pafG-CBE-3T

    Plasmid

    #220374

    Purpose

    for hA3A-nCas9 and gRNA expression in Aspergillus flavus

    Depositor

    Xue Gao

    Article

    Zhao et al J Am Chem Soc. 2023 Jan 11;145(1):413-42

    Inserts

    hA3A-nCas9
    tRNA and gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ptef1 and tRNA

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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