We narrowed to 6,889 results for: GFP expression plasmids

1. pAAVLINKv1-pCALM1-Cre-3' AHDC1

   Plasmid

   #236673

   Purpose

   3' AAVLINK plasmid for AHDC1 expression

   Depositor

   Zhonghua Lu

   Insert

   AHDC1 (AHDC1 Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   pCALM1

   Available since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAVLINKv1-pCALM1-Cre-3' DIP2A

   Plasmid

   #236651

   Purpose

   3' AAVLINK plasmid for KDIP2A expression

   Depositor

   Zhonghua Lu

   Insert

   DIP2A (DIP2A Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   pCALM1

   Available since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAVLINKv1-pCALM1-Cre-3' CDC42BPB

   Plasmid

   #236715

   Purpose

   3' AAVLINK plasmid for CDC42BPB expression

   Depositor

   Zhonghua Lu

   Insert

   CDC42BPB (CDC42BPB Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   PCalm1

   Available since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAVLINKv1-pCALM1-Cre-3' CHD2

   Plasmid

   #236659

   Purpose

   3' AAVLINK plasmid for CHD2 expression

   Depositor

   Zhonghua Lu

   Insert

   CHD2 (CHD2 Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   pCALM1

   Available since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAVLINKv1-pCALM1-Cre-3' IQGAP3

   Plasmid

   #236705

   Purpose

   3' AAVLINK plasmid for IQGAP3 expression

   Depositor

   Zhonghua Lu

   Insert

   IQGAP3 (IQGAP3 Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   pCALM1

   Available since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FgH1tUTG

   Plasmid

   #70183

   Purpose

   Inducible expression of guide RNA with fluorescent GFP reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   H1-Tet-sgrna cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV-CAG-FLEX-BFP2-(mCherry)’

   Plasmid

   #115234

   Purpose

   Lentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it

   Depositor

   Ian Wickersham

   Article

   Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

   Insert

   mTagBFP2 and mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRPN265NB-enh-MNase

   Plasmid

   #166035

   Purpose

   Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

   Depositor

   Marc Timmers

   Article

   Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

   Insert

   Glutathione-S-Transferase/enhancer/MNase
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   lac promoter / tac promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LLP778_Traptavidin-p65HSF1-CO

   Plasmid

   #211771

   Purpose

   AviTag counterpart binding domain, Traptavidin, fused to transcriptional activator p65HSF1, with sfGFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Traptavidin-p65HSF1
   

   Use

   Tags

   3xV5

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect p65HSF…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-CMV-FlaviA-mNeptune-puro

    Plasmid

    #140091

    Purpose

    Fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

    Depositor

    Jorge Luis Arias-Arias

    Article

    Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

    Insert

    FlaviA-mNeptune
    

    Use

    Lentiviral; High expression

    Tags

    mNeptune

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMW96

    Plasmid

    #178066

    Purpose

    Expression of His6-SUMO-TEV-GFP-PreScission-γ-TuNA in E. coli

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al Cell. 2020 Jan 9;180(1):165-175.e16. doi

    Insert

    CDK5RAP2 (CDK5RAP2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMIG-Flag-RIPK1-D324A

    Plasmid

    #102678

    Purpose

    retroviral expression plasmid for RIPK1-D324A

    Depositor

    Hasam Habelhah

    Article

    Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Retroviral

    Tags

    Flag and IRES-GFP

    Expression

    Mammalian

    Mutation

    CHANGE Glutamate 324 to Alanine

    Promoter

    unknown

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-CAG-F14F15S-BFP2-(mCherry)'

    Plasmid

    #115235

    Purpose

    Lentiviral transfer plasmid for expression of mTagBFP2 before Flp recombination and mCherry after it

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    mTagBFP2 and mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV_CaMKII-HyPer7

    Plasmid

    #238922

    Purpose

    Mammalian expression of HyPer7 in excitatory neurons

    Depositor

    Vsevolod Belousov

    Article

    Kalinichenko et al Redox Biol. 2023 Apr;60:102604. doi: 10.

    Insert

    HyPer7
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIalfa

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Interface-1 (b)

    Plasmid

    #204429

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    E626A T630A K668A E671A

    Promoter

    CMV

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Palpha6 and Interface-4 (a)

    Plasmid

    #204434

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    F860A Y861A Y862A Y863A Q864A R865A H866A F867A

    Promoter

    CMV

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Palpha2

    Plasmid

    #204441

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    W771A K772A K773A R774A W775A L776A Y777A W778A K…

    Promoter

    CMV

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. gh23

    Plasmid

    #106696

    Purpose

    expression of gRNA targeting POMT1, Cas9, and GFP

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    POMT1 (POMT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEMS2389

    Plasmid

    #225634

    Purpose

    AAV plasmid with Ple391 (DRD2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

    Insert

    Ple391 (DRD2 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEMS2383

    Plasmid

    #225635

    Purpose

    AAV plasmid with Ple385 (GPR6 MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

    Insert

    Ple385 (GPR6 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEMS2387

    Plasmid

    #225633

    Purpose

    AAV plasmid with Ple389 (ADORA2A MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

    Insert

    Ple389 (ADORA2A Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3 H2B-mIFP T2A Mpro (C145A)

    Plasmid

    #163080

    Purpose

    Expresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2B

    Depositor

    Xiaokun Shu

    Insert

    Inactive Mpro mutant C145A and H2B-mIFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AZ64_pE.DonorCLYBL.TS

    Plasmid

    #199228

    Purpose

    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for EGFP reporter
    

    Use

    Gene targeting donor plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1 gene promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid

    #223065

    Purpose

    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Mutation

    Three regions of SpCas9 encode type IIS restricti…

    Promoter

    Dual T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. lentiGuide-chr20-49345720-gRNA1

    Plasmid

    #232623

    Purpose

    Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.

    Depositor

    Claes Wadelius

    Article

    Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

    Insert

    S. pyogenes sgRNA cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. lentiGuide-chr20-49345720-gRNA2

    Plasmid

    #232624

    Purpose

    Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.

    Depositor

    Claes Wadelius

    Article

    Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

    Insert

    S. pyogenes sgRNA cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. lentiGuide-chr20-49345720-gRNA3

    Plasmid

    #232625

    Purpose

    Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.

    Depositor

    Claes Wadelius

    Article

    Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

    Insert

    S. pyogenes sgRNA cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3 H2B-mIFP T2A Mpro (wt)

    Plasmid

    #163079

    Purpose

    Expresses wild type Mpro (active) and a near-infrared fluorescent protein mIFP fused to H2B

    Depositor

    Xiaokun Shu

    Insert

    Wild type Mpro of SARS-CoV-2 and H2B-mIFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-Ptet-RFP-shR-rtTA

    Plasmid

    #35625

    Purpose

    Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

    Depositor

    Howard Gu

    Article

    Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

    Type

    Empty backbone

    Use

    AAV and RNAi; Tet inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ptet

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAVLINKv1-pCALM1-Cre-3' YTHDC2

    Plasmid

    #236697

    Purpose

    3' AAVLINK plasmid for YTHDC2 expression

    Depositor

    Zhonghua Lu

    Insert

    YTHDC2 (YTHDC2 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    pCALM1

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAVLINKv1-pCALM1-Cre-3' KDM2B

    Plasmid

    #236661

    Purpose

    3' AAVLINK plasmid for KDM2B expression

    Depositor

    Zhonghua Lu

    Insert

    KDM2B (KDM2B Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    pCALM1

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. IDP466

    Plasmid

    #87008

    Purpose

    genomic Sindbis virus plasmid for expression of SYNseq components

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Inserts

    myc-3xFlag-CLIP-Nrx1B-1xλN(i)
    eGFP
    

    Use

    Synthetic Biology; Sindbis virus genomic plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJUMP19-Pac

    Plasmid

    #126957

    Purpose

    Promoter acceptor vector. Minimal cargo vector; with eGFP without promoter in downstream secondary site. Allows introducing any part using BsmBI.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAVLINKv1-EF1alpha-5' KDM5B

    Plasmid

    #236648

    Purpose

    5' AAVLINK plasmid for KDM5B expression

    Depositor

    Zhonghua Lu

    Insert

    KDM5B (KDM5B Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    July 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAVLINKv1-pCALM1-Cre-3' NEXMIF

    Plasmid

    #236669

    Purpose

    3' AAVLINK plasmid for NEXMIF expression

    Depositor

    Zhonghua Lu

    Insert

    NEXMIF (NEXMIF Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    pCALM1

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAVLINKv1-pCALM1-Cre-3' KDM5C

    Plasmid

    #236671

    Purpose

    3' AAVLINK plasmid for KDM5C expression

    Depositor

    Zhonghua Lu

    Insert

    KDM5C (KDM5C Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    pCALM1

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAVLINKv1-pCALM1-Cre-3' KDM5A

    Plasmid

    #236711

    Purpose

    3' AAVLINK plasmid for KDM5A expression

    Depositor

    Zhonghua Lu

    Insert

    KDM5A (KDM5A Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    pCALM1

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAVLINKv1-pCALM1-Cre-3' ITSN1

    Plasmid

    #236717

    Purpose

    3' AAVLINK plasmid for ITSN1 expression

    Depositor

    Zhonghua Lu

    Insert

    ITSN1 (ITSN1 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    pCALM1

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAVLINKv1-pCALM1-Cre-3' KANK1

    Plasmid

    #236681

    Purpose

    3' AAVLINK plasmid for KANK1 expression

    Depositor

    Zhonghua Lu

    Insert

    KANK1 (KANK1 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    pCALM1

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAVLINKv1-pCALM1-Cre-3' KDM6A

    Plasmid

    #236689

    Purpose

    3' AAVLINK plasmid for KDM6A expression

    Depositor

    Zhonghua Lu

    Insert

    KDM6A (KDM6A Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    pCALM1

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pJUMP19-Tac

    Plasmid

    #126958

    Purpose

    Terminator acceptor vector. Minimal cargo vector; with eGFP in downstream secondary site and constitutive promoter J23100 in upstream site. Allows introducing any part using BsmBI.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pX330-Cetn1/1

    Plasmid

    #50718

    Purpose

    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Inserts

    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and hU6

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. AD59_pEP.DonorCLYBL.TS

    Plasmid

    #199227

    Purpose

    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for PuroR.T2A.EGFP selectable and reporter markers
    

    Use

    Gene targeting donor plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1 gene promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. IL1RN gRNA3

    Plasmid

    #113132

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. IL1RN gRNA4

    Plasmid

    #113133

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. IL1RN gRNA2

    Plasmid

    #113131

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pDG461

    Plasmid

    #100902

    Purpose

    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCAG-EGxxFP-Cetn1

    Plasmid

    #50717

    Purpose

    Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Insert

    Cetn1 (Cetn1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pBS513 EF1alpha-cre

    Plasmid

    #11918

    Purpose

    Expresses Cre under the control of the elongation factor-1 alpha (EF1alpha) promoter; includes upstream Kozak modification to increase expression.

    Depositor

    Brian Sauer

    Article

    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

    Insert

    cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pLV-SFFV-RTN3-WPRE-UbC-Emerald

    Plasmid

    #233467

    Purpose

    Lentiviral vector plasmid expressing human reticulon 3 (RTN3) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Evan Reid, Bart Nieuwenhuis

    Inserts

    RTN3 (RTN3 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge