We narrowed to 34,868 results for: CaS;

1. p2301y-tdtomato-dCas9_DegronDead_TPLN188

   Plasmid

   #205409

   Purpose

   dCas9 guided repressor

   Depositor

   Jennifer Nemhauser

   Article

   Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

   Insert

   dCas9_DegronDead_TPLN188
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-dCas12a-A3B

   Plasmid

   #193642

   Purpose

   Expression of base editor dCas12a-A3B in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3B, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-dCas12a-A3G

   Plasmid

   #193643

   Purpose

   Expression of base editor dCas12a-A3G in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3G, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. RCAS.Sfi myr-Akt1

   Plasmid

   #196374

   Purpose

   Akt1 with a myristoylation signal in avian expression vector

   Depositor

   Peter Vogt

   Article

   Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

   Insert

   myr-Akt1
   

   Use

   Retroviral; Avian

   Tags

   HA and myristoylation

   Expression

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. RCAS (A) Wnt3a (CT#169)

   Plasmid

   #13976

   Depositor

   Cliff Tabin

   Article

   Kengaku et al Science. 1998 May 22. 280(5367):1274-7.

   Insert

   Wnt3a (Wnt3a Mouse)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

6. FUG-T2A-Cas9

   Plasmid

   #75346

   Purpose

   Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP (via t2a)

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ-dpcoCas9-EV2.1

   Plasmid

   #158411

   Purpose

   CRISPR-dpcoCas9 fused with EDLL activator for transcriptional activation

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330 N-FKBP-Cas9

   Plasmid

   #162727

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   FKBP F36V
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. RCAS(A) cNkx2.5 (CT#800)

   Plasmid

   #13951

   Depositor

   Cliff Tabin

   Article

   Smith et al Development. 2000 Sep . 127(17):3671-81.

   Insert

   Nkx2.5 (NKX2-5 Chicken)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

10. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

    Plasmid

    #86611

    Purpose

    Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Tags

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

    Plasmid

    #183077

    Purpose

    Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c tracrRNA and crRNA ending with a HDV ribozyme
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. RCAS(A) mTcf4B (CT#1126)

    Plasmid

    #14019

    Depositor

    Cliff Tabin

    Article

    Kardon et al Dev Cell. 2003 Dec . 5(6):937-44.

    Insert

    Tcf4B (Tcf7l2 Mouse)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pSpCas9(BB)-2A-GFP-G3BP2(2)

    Plasmid

    #136061

    Purpose

    G3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP2 (G3BP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ-dAaCas12b-Act3.0

    Plasmid

    #158413

    Purpose

    CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330_ACTB-i1 sgRNA / hSpCas9

    Plasmid

    #172825

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX459-HypaCas9-TUBA1B_sgRNA

    Plasmid

    #183889

    Purpose

    pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    TUBA1B sgRNA spacer (TUBA1B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBLO43.3.-6_human ProCas9Flavi-6

    Plasmid

    #121630

    Purpose

    U6-sgRNAdest_CMV-intron_hProCas9Flavi-6_T2A Mcherry_AmpR_ColE1

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCaSpeR4-tubulin-QF#7m1

    Plasmid

    #46128

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7m1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pST1374-dCas9-GCN4

    Plasmid

    #113026

    Purpose

    Expresses dCas9-GCN4 in mammalian cells

    Depositor

    Xingxu Huang

    Article

    Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

    Insert

    dCas9-GCN4 (GCN4 S. pyogenes, Budding Yeast)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A, N863A

    Promoter

    CMV

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAGT8162 (AATG_dttLbCas12a-i_TTCG)

    Plasmid

    #192350

    Purpose

    L0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expression

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    AATG_dttLbCas12a-i_TTCG
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D156R; E925Q

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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