We narrowed to 41,246 results for: Eras
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Plasmid#198527PurposeExpression of GAL4 DNA-binding domain (BD)-FHIP fusion protein in yeast (yeast two-hybrid assays)DepositorInsertFHIP (FHIP1B Human)
TagsGAL4-DNA binding domain fragmentExpressionYeastMutationsilent mutations in codons 353 and 651 as well as…PromoterADH1Available SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-Hook1
Plasmid#198523PurposeExpression of GAL4 DNA-binding domain (BD)-Hook1 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertHook1 (HOOK1 Human)
TagsGAL4-DNA binding domain fragmentExpressionYeastMutationsilent substitution in codon 620 to eliminate int…PromoterADH1Available SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-3exo-Tetra-com-CLCN5-sp-g1
Plasmid#176236PurposePlasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-5Exo-tetra-com-hCLCN5-sp-g1
Plasmid#176237PurposePlasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-Exo-tetra-com-hCLCN5-sp-g1
Plasmid#176238PurposePlasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
NSS-GFP
Plasmid#163661PurposeExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF and TMD and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from TNF…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SNN-GFP
Plasmid#163664PurposeExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TNF in mammalian cellsDepositorInsertTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from ST,…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
NNS-GFP
Plasmid#163666PurposeExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TNF, and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from TNF…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCT-CMV-copGFP-Polß(WT)-puro
Plasmid#128665Purposemammalian expression of copGFP-Polß(WT) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagscopGFPExpressionMammalianMutationWild typePromoterCMVAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)
Plasmid#62580PurposeTranslational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.DepositorInsertSPRED1 (SPRED1 Human)
UseLuciferaseMutationmiR-21 binding site 1 mutant (TAAGCTA --> TAGA…Available SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)
Plasmid#62581PurposeTranslational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.DepositorInsertSPRED1 (SPRED1 Human)
UseLuciferaseMutationmiR-21 binding site 2 mutant (TAAGCTA --> TAGA…Available SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-eFFly-mCherry
Plasmid#104833Purpose3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detectionDepositorInsertseFFly
mCherry
UseLentiviral and LuciferaseExpressionMammalianAvailable SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
AID-C12*-dCas9-BE4max
Plasmid#216731PurposeExpresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.DepositorAvailable SinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK FKBP12(F36V)-OGT (Neo)
Plasmid#154294PurposeLentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoterDepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x HA and FKBP12(F36V)ExpressionMammalianPromoterPGKAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2-CIB1-mCherry-Rab8A
Plasmid#194333PurposeFor use in light-induced Rab8a protein inactivation through the interaction with CRY2DepositorTagsmcherryExpressionMammalianPromoterCMV IE94Available SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2 HNF4A P2 wt
Plasmid#60324PurposeHNF4A P2 promoter cloned into the pGL2-basic backbone.DepositorAvailable SinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
AID-C12*-BE4max
Plasmid#216729PurposeExpresses a BE4max base editing construct comprised of the hyperactive AID-C12* and nCas9 (Cas9 with D10A mutation) in mammalian cells.DepositorAvailable SinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
NLuc-GID4
Plasmid#200878PurposeMammalian expression of human GID4 with N-terminal NanoLuciferase fusionDepositorAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFPc1 hp47phox (S303/304/328D)
Plasmid#112245Purposeexpression of mutant p47phoxDepositorAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
FSF-GFP
Plasmid#163658PurposeExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from TFR, TMD from ST6GAL1, and luminal domain from TFR in mammalian cellsDepositorInsertTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from TFR…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only