We narrowed to 15,942 results for: LEA
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Plasmid#146482PurposeInsect Expression of DmGW182-M2-CtermDepositorAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only
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pAc5.1B-lambdaN-DmeIF4E-siRNAres-V5His6_H
Plasmid#146487PurposeInsect Expression of DmeIF4E-siRNAresDepositorInsertDmeIF4E-siRNAres
UseTagsExpressionInsectMutation3 silent mutations C411A, T531C, C652T compared t…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-M2-Cterm_H
Plasmid#146495PurposeInsect Expression of DmGW182-M2-CtermDepositorAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPop2a-V5His6_G
Plasmid#146390PurposeInsect Expression of DmPop2aDepositorAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-delDUFdelCterm_G
Plasmid#146393PurposeInsect Expression of DmGW182-delDUFdelCtermDepositorAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-delMGWdelRBD_G
Plasmid#146394PurposeInsect Expression of DmGW182-MGWdelRBDDepositorAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-delQdelMGW_G
Plasmid#146395PurposeInsect Expression of DmGW182-delQdelMGWDepositorAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
GLRX-roGFP2 in pT3TS-Dest
Plasmid#194297PurposeIn vitro transcription of the redox sensor GLRX-roGFP2 from the T3 promoter. Parton lab clone KRKDepositorInsertGLRX-roGFP2
UseIn vitro transcription of mrnaTagsExpressionMutationPromoterAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ_CydAB-mNeongreen
Plasmid#182393PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper and cydAB--mNeongreen fusionsDepositorInsertsVNp-LZ
CydAB-mNeongreen
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-p3E-GLRX-roGFP2
Plasmid#194288PurposeMultisite gateway entry clone for expression of the redox probe GLRX-roGFP2. Parton lab clone KQFDepositorInsertGLRX-roGFP2
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV sgRNA-Hic1 GFP
Plasmid#106953PurposeLentivirus encoding sgRNA targeting murine Hic1. Includes GFP marker.DepositorInsertanti-Hic1 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-mCherry-HRasCT
Plasmid#186704PurposeEncoding membrane-targeted mCherryDepositorInsertmCherry-HRasCT
UseTagsExpressionMammalianMutationPromoterAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.040
Plasmid#184974PurposeTest effect of extending a1/a2 on ADE2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v2
UseTagsExpressionYeastMutationADE2 donor P272stop, a1/a2 length extended to 27 …PromoterGal7Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.108
Plasmid#184977PurposeExpress -Eco1 LYP1 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 12
UseTagsExpressionYeastMutationLYP1 donor E27stopPromoterGal7Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.109
Plasmid#184978PurposeTest effect of extending a1/a2 on LYP1 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationLYP1 donor E27stop, a1/a2 length extended to 27 bpPromoterGal7Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.107
Plasmid#184976PurposeTest effect of extending a1/a2 on CAN1 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationCAN1 donor G444stop, a1/a2 length extended to 27 …PromoterGal7Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.106
Plasmid#184975PurposeExpress -Eco1 CAN1 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 12
UseTagsExpressionYeastMutationCAN1 donor G444stopPromoterGal7Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.111
Plasmid#184980PurposeTest effect of extending a1/a2 on TRP2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationTRP2 donor E64stop, a1/a2 length extended to 27 bpPromoterGal7Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.0
Plasmid#190795PurposePRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning processDepositorInsertPRE-Hsp70Bb-Cas9-T2A-eGFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.037
Plasmid#184966PurposeExpress -Eco1 dead RT and ncRNA in yeasstDepositorInsertEco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT
UseTagsExpressionYeastMutationHuman codon optimized RT, YXDD catalytic region m…PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.112
Plasmid#184981PurposeExpress -Eco1 FAA1 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12
UseTagsExpressionYeastMutationFAA1 donor P233stopPromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.113
Plasmid#184982PurposeTest effect of extending a1/a2 on FAA1 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationFAA1 donor P233stop, a1/a2 length extended to 27 …PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.002
Plasmid#184972PurposeExpress -Eco1 ADE2 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 12
UseTagsExpressionYeastMutationADE2 donor P272stopPromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.110
Plasmid#184979PurposeExpress -Eco1 TRP2 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12
UseTagsExpressionYeastMutationTRP2 donor E64stopPromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.039
Plasmid#184973PurposeTest effect of extending a1/a2 on ADE2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationADE2 donor P272stop, a1/a2 length extended to 27 …PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
hsLEMD2-pX330
Plasmid#179511PurposeEncodes gRNA for human LEMD2DepositorInsertgRNA for LEMD2
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7
Plasmid#186753PurposeA basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.DepositorTypeEmpty backboneUseSynthetic Biology; Cloning backboneTagsExpressionMutationPromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-NLSlacZ-stop-L2
Plasmid#186358PurposeEntry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.DepositorInsertlacZ (lacZ E. coli, Synthetic)
UseExpression of nuclear lacz reporterTagsNuclear localization signalExpressionMutationPromoterAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.407
Plasmid#187204PurposeExpresses retron Eco1 ncRNA v32, and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v32
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7/LacAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.408
Plasmid#187205PurposeExpresses retron Eco1 ncRNA v35, and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v35
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7/LacAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB438
Plasmid#185092PurposeGAL1::Rfa1[1-337, 344-621]-GFP with NLS removedDepositorInsertRFA1
UseTagsExpressionYeastMutationRfa1 with amino acids 338-343 deletedPromoterGAL1Available SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB372
Plasmid#185084PurposeGAL::Rfa1[330-621]-GFP under control of GAL1 promoterDepositorInsertRFA1
UseTagsGFPExpressionYeastMutationRfa1 amino acids 330-621PromoterGAL1Available SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)
Plasmid#158771PurposeExpression of synaptotagmin 1 variant with self-cleaving C2 domain.DepositorInsertSynaptotagmin 1 1-NS3/4a (Syt1 Mouse)
UseLentiviralTagsExpressionMutationFlexible linkers, ADVVCC/QMSY cleavage sitePromoterAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
NCH2exp
Plasmid#131019PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH2
UseTagseGFP-FLAG-HAExpressionPlantMutationPromoterP35SAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MNEAP1ec
Plasmid#131020PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMNEAP1
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationPromoterAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MNEAP1exp
Plasmid#131021PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMNEAP1
UseTagseGFP-FLAG-HAExpressionPlantMutationPromoterP35SAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MKAKU41ec
Plasmid#131014PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMKAKU41
UseGateway entry vectorTagsmCherry-FLAG-HAExpressionMutationPromoterAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
MKAKU41exp
Plasmid#131015PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMKAKU41
UseTagsmCherry-FLAG-HAExpressionPlantMutationPromoterP35SAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
NCH1ec
Plasmid#131016PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH1
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationPromoterAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
NCH1exp
Plasmid#131017PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH1
UseTagseGFP-FLAG-HAExpressionPlantMutationPromoterP35SAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
NCH2ec
Plasmid#131018PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH2
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationPromoterAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAc5.1B-lambdaN-HA-DmPAN3-dsRNAres_Y
Plasmid#148080PurposeInsect Expression of DmPAN3-dsRNAresDepositorAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-delUBAdelQrich_G
Plasmid#146396PurposeInsect Expression of DmGW182-delUBAdelQrichDepositorAvailable SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-SpRYgest_targetplasmid (KAC833)
Plasmid#181748PurposepUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.DepositorTypeEmpty backboneUseSubstrate plasmidTagsExpressionMutationPromoterAvailable SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Pigb-V5
Plasmid#175160PurposeLentiviral expression of mouse Pigb-V5DepositorAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-tRNA-Gly
Plasmid#174317PurposeFor in vitro transcription of mouse tRNAsDepositorInserttRNA-gly
UseTagsExpressionBacterialMutationPromoterT7Available SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc397
Plasmid#161955PurposeDemonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHHDepositorInsertpSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
UseTagsExpressionBacterialMutationPromoterPBADAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC-Hybrid1
Plasmid#173469PurposeN-terminal region of PGRMC2 fused to the transmembrane and C-terminal region of PGRMC1DepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC-Hybrid2
Plasmid#173470PurposeN-terminal and transmembrane region of PGRMC2 fused to the C-terminal region of PGRMC1DepositorUseTagsFLAGExpressionMammalianMutationPromoterAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC-Hybrid3
Plasmid#173471PurposeN-terminal region of PGRMC1 fused to the transmembrane and C-terminal region of PGRMC2DepositorUseTagsFLAGExpressionMammalianMutationPromoterAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only