We narrowed to 5,724 results for: Chia

1. pAJM.657

   Plasmid

   #108525

   Purpose

   CymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   CymRAM + PCymRC-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   CymR: S110G A171V

   Promoter

   Available since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMON-HsNMT1

   Plasmid

   #67475

   Purpose

   Expression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteria

   Depositor

   Richard Kahn

   Article

   Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

   Insert

   HsNMT1 (NMT1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCKmatBC

   Plasmid

   #138587

   Purpose

   matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat

   Depositor

   Markus Jeschek

   Article

   Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

   Insert

   bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   matB contains native GUG start codon

   Promoter

   Plac promoter

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF278

   Plasmid

   #167673

   Purpose

   Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLG027 (qatABCD)

   Plasmid

   #157905

   Purpose

   Expresses the qatABCD defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   qatABCD (ATPase + unknown protein + QueC homolog + TatD DNase)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAJM.677

   Plasmid

   #108530

   Purpose

   AraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   AraCAM + AraE + PBAD-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   AraC: L133F E165L E169G C280L

   Promoter

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAJM.474

   Plasmid

   #108526

   Purpose

   LuxR + PLuxB-YFP Reporter. OC6 sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   LuxR + PLuxB-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   VanR: T49I A111V P179S

   Promoter

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1a-mCherry-MinD-IRES-Puro

   Plasmid

   #213112

   Purpose

   Expression of mCherry-MinD in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   mCherry-MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4046768

   Plasmid

   #145763

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pSR59.4

    Plasmid

    #63175

    Purpose

    Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    OmpR (ompR )
    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBXNH3CA_MBP

    Plasmid

    #132700

    Purpose

    expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    Maltose binding Protein
    

    Use

    Tags

    Avi and His

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBW-m4046959

    Plasmid

    #145722

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pBbS8c-ddcpf1-rfp

    Plasmid

    #153038

    Purpose

    For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E993A

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pScI_dCas9-CDA-UL

    Plasmid

    #108551

    Purpose

    Bacterial Target-AID UGI-LVA vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    dCas9-PmCDA1-UGI-LVA
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    lambda OR

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSF280

    Plasmid

    #167675

    Purpose

    Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL4393 (pClpX_Eco)

    Plasmid

    #200907

    Purpose

    Expresses human codon-optimized E. coli ClpX from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    EcoClpX
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas12f1

    Plasmid

    #171182

    Purpose

    Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    Cas12f1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cas9 promoter from Streptococcus pyogenes

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2CT-His-MBP-Lsh_C2c2_WT

    Plasmid

    #83487

    Purpose

    Bacterial expression of WT L. shahii C2c2 CRISPR effector

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lsh_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. SupD v2

    Plasmid

    #198598

    Purpose

    Plasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SupD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAJM.336

    Plasmid

    #108528

    Purpose

    LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    LacIAM + PTac-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LacI Q18M A47V F161Y

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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