We narrowed to 22,471 results for: ESC

1. pClathrin-miRFP670nano3

   Plasmid

   #184667

   Purpose

   Clathrin labelled with near-infrared fluorescent protein miRFP670nano3

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   miRFP670nano3-clathrin light chain
   

   Use

   Tags

   miRFP670nano3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1/Puro-CAG-ASAP1

   Plasmid

   #52519

   Purpose

   For mammalian expression of the fluorescent voltage sensor ASAP1

   Depositor

   Michael Lin

   Article

   St-Pierre et al Nat Neurosci. 2014 Apr 22. doi: 10.1038/

   Insert

   Accelerated Sensor of Action Potentials 1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFast-FT-N1

   Plasmid

   #31910

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Fast-FT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

   Promoter

   CMV

   Available since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-Streptavidin-K121R

   Plasmid

   #89880

   Purpose

   Streptavidin that has unimpaired biotin binding after dye labeling

   Depositor

   Mark Howarth

   Article

   Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

   Insert

   Streptavidin-K121R-Glutamate_Tag
   

   Use

   Tags

   6 glutamate tag (C terminal on insert)

   Expression

   Bacterial

   Mutation

   K121R

   Promoter

   Available since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMito-Electra2

   Plasmid

   #184927

   Purpose

   Mitochondrial labeling with blue fluorescent protein Electra2 fused to partial COX8A

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   COX8A-Electra2
   

   Use

   Tags

   Electra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLD1 translation factors

   Plasmid

   #117760

   Purpose

   BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli

   Depositor

   Anthony Forster

   Article

   Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   Translation factor cistrons 1-13
   

   Use

   Escherichia coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC1-HyPerRed-mito

   Plasmid

   #60247

   Purpose

   Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

   Insert

   HyPerRed-mito
   

   Use

   Tags

   Mitochondrial targeting signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLD3 translation factors

   Plasmid

   #117762

   Purpose

   BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

   Depositor

   Anthony Forster

   Article

   Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   Translation factor cistrons 22-30
   

   Use

   Escherichia coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLD2 translation factors

   Plasmid

   #117761

   Purpose

   BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coli

   Depositor

   Anthony Forster

   Article

   Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   Translation factor cistrons 14-21
   

   Use

   Escherichia coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-mRuby2-Gephyrin.FingR-IL2RGTC

    Plasmid

    #125696

    Purpose

    Global labeling of inhibitory synapses (red fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    mRuby2-Gephyrin.FingR-IL2RGTC
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-smURFP-IRES-HO-1

    Plasmid

    #80345

    Purpose

    Expresses smURFP and Heme Oxygenase-1 in mammalian cells.

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    smURFP
    HO-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and IRES

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPAmCherry2-C1

    Plasmid

    #31932

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18L/K69N/L84F/A147T/S148L/I165L/Q167A/L169T/A179…

    Promoter

    CMV

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSETA-qmTQ2-ATP

    Plasmid

    #225247

    Purpose

    Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-ATP
    

    Use

    Tags

    6XHis, T7 tag, XpressTM tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTRE-Medium-FT

    Plasmid

    #31914

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Medium-FT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

    Promoter

    PTight Tet-responsive PminCMVd

    Available since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSF11-wElectra2

    Plasmid

    #184933

    Purpose

    Codon optimized blue fluorescent protein Electra2 in C.elegans expresison plasmid

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    tag-168

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCdrA-gfpC

    Plasmid

    #111614

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS41K-mScarlet-I-mNeonGreen (pKBJ010)

    Plasmid

    #173461

    Purpose

    C-SWAT donor for Seamless tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-I-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMedium-FT-N1

    Plasmid

    #31911

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Medium-FT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

    Promoter

    CMV

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSEM233 - [Pmlc-1 | tagRFP-T | cbr-tbb-2 UTR]

    Plasmid

    #159899

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | tagRFP | cbr-tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET24a-AlkB deltaN11

    Plasmid

    #73622

    Purpose

    Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

    Depositor

    Catherine Drennan

    Article

    Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    AlkB (alkB E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deletion of first 11 AA

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only