We narrowed to 2,494 results for: T2A

1. pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

   Plasmid

   #166133

   Purpose

   This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

   Insert

   hU6-gRNA and hH1-gRNA targeting cldn1 gene
   

   Use

   CRISPR and Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

   Plasmid

   #166134

   Purpose

   This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

   Insert

   hU6-gRNA and hH1-gRNA targeting firefly luciferase gene
   

   Use

   CRISPR and Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL

   Plasmid

   #172208

   Purpose

   AAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1S promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

   Depositor

   Garrett Kaas

   Article

   Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

   Insert

   HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL
   

   Use

   AAV and Mouse Targeting; Tale

   Tags

   HA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL

   Plasmid

   #172200

   Purpose

   AAV sequence-specific control vector for TALE-Tet1S-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

   Depositor

   Garrett Kaas

   Article

   Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

   Insert

   HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL
   

   Use

   AAV and Mouse Targeting; Tale

   Tags

   HA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   transcription regulatory domain removed

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS-SID4X_2A_EGFP_W3SL

   Plasmid

   #172205

   Purpose

   AAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1FL promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

   Depositor

   Garrett Kaas

   Article

   Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

   Insert

   HA-TALE(NG)-Tet1FL-SID4X-NLS_2A_EGFP_W3SL
   

   Use

   AAV and Mouse Targeting; Tale

   Tags

   HA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPN459

   Plasmid

   #137875

   Purpose

   PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinR

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TCF4 (TCF4 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. StopPR epegRNA library (lAC002)

   Pooled Library

   #227706

   Purpose

   CRISPR guide RNA library for prime editing

   Depositor

   Brittany Adamson

   Article

   Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

   Expression

   Mammalian

   Use

   Lentiviral

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

8. MOBE3

   Plasmid

   #219943

   Purpose

   Mammalian expression of MOBE3 protein (apt-CBE-SL3_PT2A_apt-ABE8e)

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   apt-CBE-SL3_PT2A_apt-ABE8e
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. MOBE4

   Plasmid

   #219944

   Purpose

   Mammalian expression of MOBE4 protein (apt-CBE-SL3_PT2A_apt-ABE8.20)

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   apt-CBE-SL3_PT2A_apt-ABE8.20
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MOBE2

    Plasmid

    #219942

    Purpose

    Mammalian expression of MOBE2 protein (apt-CBE-3'end_PT2A_apt-ABE8.20)

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    apt-CBE-3'end_PT2A_apt-ABE8.20
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSN993

    Plasmid

    #219961

    Purpose

    Expresses CMT2A mutant version of human KIF1Bß in C. elegans

    Depositor

    Shinsuke Niwa

    Insert

    unc-104 promoter::human KIF1Bß(Q98L) (KIF1B Human, Nematode)
    

    Expression

    Worm

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACEBac1-ZZ-TEV-MZT2-3C-mEGFP

    Plasmid

    #178074

    Purpose

    Insect cell expression of ZZ-TEV-MZT2-3C-mEGFP

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    MZT2A (MZT2A Human)
    

    Tags

    PreScission-mEGFP and ZZ-TEV

    Expression

    Insect

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Self-targeting epegRNA library, +5 G>H (lDS004)

    Pooled Library

    #227707

    Purpose

    CRISPR guide RNA library for prime editing

    Depositor

    Brittany Adamson

    Article

    Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

    Expression

    Mammalian

    Use

    Lentiviral

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

14. Self-targeting epegRNA library, Tiled edits (lRM001)

    Pooled Library

    #227708

    Purpose

    CRISPR guide RNA library for prime editing

    Depositor

    Brittany Adamson

    Article

    Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

    Expression

    Mammalian

    Use

    Lentiviral

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

15. pAAVS1-Neo-CAG-M2rtTA-H2BmNeon (donorAB-mNeon)

    Plasmid

    #85799

    Purpose

    donorAB-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP

    Depositor

    Roland Friedel

    Article

    Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

    Insert

    PPP1R12C (PPP1R12C Human)
    

    Use

    Human targeting

    Mutation

    homology arms for knock-in into first intron

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-EF1a-eFFly-mCherry

    Plasmid

    #104833

    Purpose

    3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

    Depositor

    Irmela Jeremias

    Article

    Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

    Inserts

    eFFly
    mCherry
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTet-O-Ngn2-puro

    Plasmid

    #52047

    Purpose

    3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

    Depositor

    Marius Wernig

    Article

    Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

    Insert

    Ngn2 (Neurog2 Mouse)
    

    Use

    Lentiviral

    Tags

    T2A-and puromycin resistant cassette

    Expression

    Mammalian

    Promoter

    tet-on

    Available Since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-EF1a-mCherry-EGFP-LC3B

    Plasmid

    #170446

    Purpose

    Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    mCherry-GFP-LC3 (MAP1LC3B Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pgRNA-CKB

    Plasmid

    #73501

    Purpose

    gRNA expression vector (with mKate2)

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    mKate2-T2A-Bsd
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. PE7_AAVS1_knockin_HDR_donor

    Plasmid

    #223001

    Purpose

    HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Hdr donor

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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