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BMP4_pLX307

Plasmid

#98321

Purpose

Lentiviral expression of BMP4

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

BMP4 (BMP4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMLS288

Plasmid

#73735

Purpose

SapTrap flexible linker N-terminal connector donor plasmid

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Flexible Linker

Use

CRISPR

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS287

Plasmid

#73730

Purpose

SapTrap flexible linker C-terminal connector donor plasmid

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Flexible Linker

Use

CRISPR

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAd-Track Flag GCN5 Y621A/P622A

Plasmid

#14425

Depositor

Pere Puigserver

Publication

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

GCN5 Y621A/P622A (KAT2A Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

Y621A, P622A. Catalytically inactive acetyltransf…

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pSC101-Donor

Plasmid

#140630

Purpose

Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

Depositor

Sheng Yang

Publication

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

LE, RE, KanR, temperature sensitive pSC101 origin of replication

Use

CRISPR; Transposon

Expression

Bacterial

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomaGCaMP6f2

Plasmid

#158756

Purpose

Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

Depositor

Edward Boyden

Publication

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP6f2

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-cGASsh4

Plasmid

#127646

Purpose

Knock-down of human cGAS

Depositor

Nicolas Manel

Publication

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

cGAS shRNA (CGAS Human)

Use

Lentiviral

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA-GRK3

Plasmid

#32689

Depositor

Robert Lefkowitz

Publication

Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

Insert

GRK3 (GRK3 Bovine)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pmRFP-SUMO1 GG

Plasmid

#13990

Depositor

Jonathan Chernoff

Publication

Dadke et al Nat Cell Biol. 2007 Jan . 9(1):80-85.

Insert

SUMO1 GG (Sumo1 Mouse)

Tags

mRFP

Expression

Mammalian

Mutation

C-terminal truncation. Cannot ligate to substrate…

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pEGFPC3-mSufu

Plasmid

#65431

Purpose

Expresses mouse Sufu protein in mammalian cells

Depositor

Aimin Liu

Publication

Zeng et al PLoS One. 2010 Dec 29;5(12):e15900. doi:

Insert

Suppressor of Fused (Sufu Mouse)

Tags

EGFP

Expression

Mammalian

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMU-TPr

Plasmid

#61189

Purpose

Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtTIP1-1

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCDF-His-mTET1CD

Plasmid

#81053

Purpose

bacterial expression of the catalytic domain of murine TET1

Depositor

Primo Schaer

Publication

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

catalytic domain amino acid 1367-2057

Promoter

T7

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-3HA-His3MX6

Plasmid

#41600

Depositor

John Pringle

Publication

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Available since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTorPE-G-GECO1

Plasmid

#32466

Depositor

Robert Campbell

Publication

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFQAQLGGL…

Expression

Bacterial

Mutation

GCaMP3 K119I/L173Q/S404G/E430V

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMKO.1 GFP B55 alpha shRNA 3-1

Plasmid

#10679

Depositor

William Hahn

Publication

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B55 alpha shRNA (PPP2R2A Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pENTR-JNKKTRClover

Plasmid

#59139

Purpose

ORF encoding JNK KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Publication

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Mouse, Human)

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-15b-UbcH5b/2419

Plasmid

#11475

Depositor

Allan Weissman

Publication

Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

Insert

UbcH5b (UBE2D2 Human)

Expression

Bacterial

Mutation

n/a

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
psgRNAv1-empty

Plasmid

#171611

Purpose

sgRNA_v1 plasmid for AsCas12f1 in bacteria

Depositor

Quanjiang Ji

Publication

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1_sgRNA_1

Expression

Bacterial

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmScarlet-HSP70

Plasmid

#163790

Purpose

Expresses mScarlet-Hsp70 in mammalian cells

Depositor

Vincent Timmerman

Publication

Adriaenssens et al Sci Rep. 2020 May 29;10(1):8755. doi: 10

Insert

HSP70 (HSPA1A Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLX303-ABI1-dCas9

Plasmid

#154474

Purpose

Expresses ABI1 fused to the N terminus of dCas9

Depositor

Mikko Taipale

Publication

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ABI1 (ABI1 Mustard Weed)

Use

Lentiviral

Expression

Mammalian

Mutation

Includes ABI1 aa 126-423, D143A

Promoter

CMV

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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