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EC70188

Plasmid

#209449

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

HvU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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LRG2.1_Puro

Plasmid

#125594

Purpose

Lentiviral expression of sgRNA with GFP and puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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PyronicSF-mRuby2/pBI-CMV1

Plasmid

#124830

Purpose

Highly resposive GFP-based pyruvate nanosensor.

Depositor

Luis Felipe Barros

Article

Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

Inserts

PyronicSF
mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K29

Plasmid

#22903

Depositor

Sandra Weller

Article

Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K29 only, all other lysines mutated to arginines

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pFLAG-Egfr(Velvet)

Plasmid

#18788

Depositor

Bruce Beutler

Article

Du et al Genetics. 2004 Jan . 166(1):331-40.

Insert

epidermal growth factor receptor (Egfr Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

D833G; signal sequence removed (aa1-24)

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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piggyBac CMV-GFP-FRT

Plasmid

#92078

Purpose

production of transgenic animal and recombination using flipase

Depositor

Jae Yong Han

Article

Lee et al FASEB J. 2016 Feb;30(2):555-63. doi: 10.

Insert

CMV-eGFP and TK-Neo between FRT3 and FRT

Use

Piggybac

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-mitoLbNOX-Flag

Plasmid

#234985

Purpose

Expresses mitochondrial LbNOX in human cell lines

Depositor

Denis Titov

Article

Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

Insert

mito-LbNOX

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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LVXN-Neo-NSD2-E1099K

Plasmid

#86011

Purpose

express NSD2 E1099K mutant in mammalian cells

Depositor

Darrin Stuart

Article

Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

Insert

NSD2 E1099K mutant (NSD2 Human)

Tags

Flag tag D Y K D D D D K

Expression

Mammalian

Mutation

E1099K; K1002R (please see depositor comments bel…

Promoter

CMV

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only
pH2B-miRFP713-N1

Plasmid

#197230

Purpose

Expresses the fusion protein of H2B-miRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

H2B-miRFP713

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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CBF:H2B-Venus

Plasmid

#44211

Depositor

Anna-Katerina Hadjantonakis

Article

Nowotschin et al BMC Dev Biol. 2013 Apr 25;13:15. doi: 10

Insert

CBF1-Responsive-Element

Expression

Mammalian

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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