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pLH-sgRNA-Sirius-8XMS2
Plasmid#121939PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minPro
Plasmid#211517PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSV40 and minProAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPUR-mU6-sgRNA-Sirius-8XPP7
Plasmid#121943PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XPP7
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromotermouse U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pHAGE-TO-dCas9-P2A-HSA
Plasmid#121936PurposeCRISPR-Sirius plasmidDepositorInsertdCas9-P2A-HSA
UseCRISPR and LentiviralTagsHSAExpressionMammalianMutationPromoterCMV-TOAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSIIhyg-rtTA
Plasmid#139480PurposeExpresses rtTA, Tet-ON reverse tetracycline-controlled trans-activator in mammalian cells.DepositorInsertrtTA, Tet-ON reverse transactivator
UseLentiviralTagsExpressionMammalianMutationPromoterHuman EF1a promoterAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro Xlone-eGFP
Plasmid#136936Purposedonor plasmid for inducible transgene expression in human cellsDepositorInsertall-in-one tet-on system
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag FKHR AAA mutant
Plasmid#13508DepositorInsertFKHR AAA (FOXO1 Human)
UseTagsFlagExpressionMammalianMutationT24A S256A S319APromoterAvailable sinceDec. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
negActinin-sstFRET-GR
Plasmid#83417PurposeFRET-based actinin tension sensor (negative control)DepositorInsertnegActinin-sstFRET
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
P[acman]-Alpha2
Plasmid#165859PurposeAlpha2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nucGFP11-3xControlgRNA
Plasmid#224568PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)ExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minTK
Plasmid#211516PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSV40 and minTKAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSC-SP-PW-NepX (aka: pBOB-NEPX)
Plasmid#12340DepositorInsertNEP-X (MME Human)
UseLentiviralTagsExpressionMammalianMutationE585V (inactive mutant)PromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pB-Neo-mtkeima
Plasmid#210338PurposeOverexpression of mtkeimaDepositorInsertmtkeima
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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RhoC FLARE.sc mCer, mVenus - wt
Plasmid#65071PurposeRhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoCDepositorInsertRBD, mCerulean, mVenus, RhoC
UseTagsExpressionBacterial, Insect, and Mamm…MutationT153M in mVenusPromoterCMV, p10Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-hPREP
Plasmid#179387PurposeExpress human prolyl oligopeptidase (tagged) in bacterial cellsDepositorInserthuman prolyl oligopeptidase (PREP Human)
UseTagsGSTExpressionBacterialMutationPromoterTACAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only
