We narrowed to 7,220 results for: 11

1. plk1 T210D

   Plasmid

   #132964

   Purpose

   To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

   Depositor

   Michael Yaffe

   Article

   Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

   Insert

   polo-like kinase 1 (PLK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T210D

   Promoter

   Available since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV-U6+27-tCorn

   Plasmid

   #106233

   Purpose

   Express tRNA-Corn as a reporter of U6 promoter activity in mammalian cells

   Depositor

   Samie Jaffrey

   Article

   Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

   Insert

   tRNA-Corn
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-HA-rat Nrxn1beta AS4(-)

   Plasmid

   #58267

   Purpose

   Expression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)

   Depositor

   Peter Scheiffele

   Article

   Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

   Insert

   Nrxn1beta AS4(-) (Nrxn1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   see comments

   Promoter

   CAG

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. plk1 T210A

   Plasmid

   #132965

   Purpose

   To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

   Depositor

   Michael Yaffe

   Article

   Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

   Insert

   polo-like kinase 1 T210A (PLK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T210A

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FLAG-CBLB

   Plasmid

   #192082

   Purpose

   Mammalian expression of human CBLB with FLAG tag

   Depositor

   Patrick Concannon

   Article

   Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

   Insert

   CBLB (CBLB Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-beta-3

   Plasmid

   #27289

   Depositor

   Timothy Springer

   Article

   Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

   Insert

   integrin beta 3 (ITGB3 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pB-Neo-mtkeima

   Plasmid

   #210338

   Purpose

   Overexpression of mtkeima

   Depositor

   Li Yu

   Article

   Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

   Insert

   mtkeima
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSpCas9n(BB) (PX460)

   Plasmid

   #48873

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9n
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMSP1E2D1

   Plasmid

   #161606

   Purpose

   Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

   Insert

   MSP1E2D1
   

   Use

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeat of h…

   Promoter

   T7

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. UbG76V-eGFP-V5His_20aa

    Plasmid

    #23970

    Depositor

    Nico Dantuma

    Article

    Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

    Insert

    Ub-G76V-eGFP-V5HIS_20aa
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    G76V mutation in Ubiquitin. Ub fused to N-termin…

    Promoter

    Available since

    March 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMSCVpuroATT

    Plasmid

    #47539

    Purpose

    Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)

    Depositor

    David Weinstock

    Article

    Shindoh et al PLoS One. 2012;7(11):e49201. doi: 10.137

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pLX301-ZIM3-KRAB-PYL1

    Plasmid

    #154761

    Purpose

    Expresses ZIM3 KRAB domain fused to PYL1

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    CMV

    Available since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. 2.2

    Plasmid

    #34885

    Depositor

    Irvin Chen

    Article

    Pariente et al Mol Ther. 2007 Nov;15(11):1973-81. Epub

    Insert

    Sindbis virus envelope protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    SGN UTR in E1 domain

    Promoter

    CMV

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV BAF170-Flag

    Plasmid

    #19142

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 (SMARCC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIntegrase_8

    Plasmid

    #60579

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_8
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ECFP-Bak

    Plasmid

    #31501

    Depositor

    Richard Youle

    Article

    Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

    Insert

    human Bak (BAK1 Human)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIntegrase_3

    Plasmid

    #60575

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pReporter_8

    Plasmid

    #60568

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_8
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIntegrase_4

    Plasmid

    #60576

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX459V2.0-HypaCas9-mRuby2

    Plasmid

    #183872

    Purpose

    pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    HypaCas9-P2A-mRuby2-T2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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