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We narrowed to 4,239 results for: crispr system

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Showing: 2161 - 2180 of 4239 results

  11. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

    Plasmid
    #100597
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)
    Depositor
    Akitsu Hotta
    Article
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available Since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100597-plasmid-map-sequence-id-207349

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  12. pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113698
    Purpose
    Dox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Available Since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113698-plasmid-map-sequence-id-232033

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  14. pCas9_sgRNA_0

    Plasmid
    #70763
    Purpose
    expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10
    Inserts
    U6 promoter
    cas9
    tnos terminator
    Use
    Self-replicating in ustilago maydis, conferring c…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U6 from Ustilago maydis and synthetic otef promot…
    Available Since
    Nov. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    70763-plasmid-map-sequence-id-240922

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  17. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

    Plasmid
    #100596
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)
    Depositor
    Akitsu Hotta
    Article
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available Since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100596-plasmid-map-sequence-id-207339

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  20. pNOC_dfnCas12a-KRAB_crRNA NC

    Plasmid
    #176262
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176262-plasmid-map-sequence-id-346962

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Showing: 2161 - 2180 of 4239 results