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pCEV-G4-Km

Plasmid

#46819

Purpose

TEF1 and HXT7 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Publication

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and HXT7

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pX330S-5

Plasmid

#58781

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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4xHRE_YB TATA-sfGFP-CMV_dsRed

Plasmid

#117399

Purpose

sfGFP expressed from hypoxia-inducible YB_TATA promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Publication

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Expression

Mammalian

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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863 pSG5L HA NES PTEN

Plasmid

#10932

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Tags

HA and NES

Expression

Mammalian

Available since

Jan. 3, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pM-CBE

Plasmid

#196293

Purpose

35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP

Depositor

Zhenghe Li

Publication

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV M antigenome encoding CBE in place of viral GP

Use

CRISPR

Tags

3×flag

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMAL-p2X

Plasmid

#75287

Purpose

Expresses proteins in the periplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Publication

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available since

July 18, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pSUPER retro puro GFP shRNA

Plasmid

#30519

Depositor

John Gurdon

Publication

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Insert

GFP shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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817 pSG5L HA PTEN G129R

Plasmid

#10748

Depositor

William Sellers

Publication

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

G129R

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
p426 103Q GPD

Plasmid

#1184

Depositor

Susan Lindquist

Publication

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 103Q (HTT Human)

Tags

GFP

Expression

Yeast

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
M13KO7-ΔM13-ΔgeneIII-ΔgeneVI

Plasmid

#80840

Purpose

Modified helper phage

Depositor

Mark Isalan

Publication

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene II
M13 gene X
M13 gene V
M13 gene VII
M13 gene IX
M13 gene VIII
M13 gene I
M13 gene XI
M13 gene IV

Expression

Bacterial

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast

Plasmid

#205565

Purpose

Express human NEDD4L (delta C2 isoform)

Depositor

Michael Elowitz

Publication

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

NEDD4L (NEDD4L Human)

Use

Piggybac

Expression

Mammalian

Mutation

delta C2 isoform

Promoter

EF1

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA_DNMT3b

Plasmid

#41823

Purpose

Expresses a guide RNA (gRNA) to target DNMT3b for genome engineering

Depositor

George Church

Publication

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_DNMT3b (DNMT3B Human)

Use

CRISPR

Expression

Mammalian

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only
BBa_J72214-BBa_J72090

Plasmid

#40782

Depositor

Christopher Anderson

Publication

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

VioABCDE

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCAG APX W41F dimeric (mito matrix)

Plasmid

#40307

Depositor

Alice Ting

Publication

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

pea APX W41F (dimeric)

Tags

Flag

Mutation

W41F mutation.

Promoter

CAG

Available since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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VV229: GCaMP6f in fck

Plasmid

#58514

Purpose

Expresses GCaMP6f under the a-CamKII promoter

Depositor

Adam Cohen

Publication

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

GCaMP6f

Use

Lentiviral

Tags

C-terminal FCYENEV (ER export motif); this was no…

Expression

Mammalian

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-CWB-cyan pre-eGRASP(p30)

Plasmid

#111586

Purpose

An AAV vector that expresses cyan pre-eGRASP(p30) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Publication

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p30)

Use

AAV

Promoter

CaMKIIa

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHSB04X

Plasmid

#117258

Purpose

Genome editing for Lactobacillus brevis ATCC367

Depositor

Sheng Yang

Publication

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019

Use

Other

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-SpCas9-HF1

Plasmid

#108301

Purpose

pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pX458-DsRed2

Plasmid

#112219

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

Depositor

Yinming Liang

Publication

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

DsRed2

Use

CRISPR

Expression

Mammalian

Mutation

DsRed2 contains a silent nucleotide substitution …

Available since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pnsgRNA

Plasmid

#172717

Purpose

Delivery of guide RNA for prime editing

Depositor

Tilmann Weber

Publication

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

J23119-gRNA

Expression

Bacterial

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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