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Plasmid#47574PurposeBacterial expression plasmid for GST-3xFlag-tagged JNK1a1DepositorInsertJNK1a1 (MAPK8 Human)
UseTags3xFlag and GSTExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ERKKTRClover
Plasmid#59138PurposeORF encoding ERK KTR mClover flanked by Gateway sequencesDepositorInsertERK Kinase Translocation Reporter (MAPK1 Human, Mouse)
UseTagsExpressionMutationPromoterAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr-mKO-MK2
Plasmid#115492PurposeExpression vector for the p38 activity reporter mKO-MK2 in mammalian cellsDepositorInsertmKO-MK2 (MAPKAPK2 Human)
UseTagsmonomeric Kusabira Orange (mKO)ExpressionMammalianMutationPromoterCAGAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp BFP-Erk D319N
Plasmid#102350PurposeLentiviral expressing translational fusion between blue fluorescent protein and Erk2 bearing the D319N mutationDepositorInsertErk2 (Mapk1 Mouse)
UseLentiviralTagsBFPExpressionMutationAspartic acid 319 was mutated to asparaginePromoterSFFVAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Tet-MKK6(DD)-Puro
Plasmid#86094PurposeTet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vectorDepositorInsertMKK6 (MAP2K6 Human)
UseLentiviralTagsMyc tagExpressionMammalianMutationSerine 207 and Threonine 211 both changed to Aspa…PromoterCMV/TetOAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MKK1K97A
Plasmid#107974Purposeexpression of a dominant negative MAP Kinase KinaseDepositorInsertMKK1 K97A (MAP2K1 Human)
UseTagsExpressionMammalianMutationdominant negative; see Depositor Comments belowPromoterCMVAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7B2Jnk1a1
Plasmid#19726DepositorUseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7B2Jnk3a2
Plasmid#19729DepositorUseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk1a1MKK7B2
Plasmid#19731DepositorUseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MKK1
Plasmid#107973Purposeexpression of MAP Kinase KinaseDepositorInsertMKK1 (MAP2K1 Human)
UseTagsExpressionMammalianMutationSee depositor comments below.PromoterCMVAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7B2Jnk1a1(APF)
Plasmid#19730DepositorUseTagsFlagExpressionMammalianMutationreplaced dual phosporylation motif Thr(1959)-Pro-…PromoterAvailable sinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-1 MKK4(EE)-MKK7a1(EE)
Plasmid#47580PurposeBacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1DepositorUseTagsExpressionBacterialMutationLacks the first 36 residues of the RefSeq protein…PromoterAvailable sinceSept. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNJP
Plasmid#115494PurposeExpression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cellsDepositorUseTagsiRFP, mCherry, and mKOExpressionMammalianMutationmCherry S227F, mCherry G229R, mKO V211GPromoterCAGAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
R777-E289 Hs.RPS6KA1
Plasmid#70573PurposeGateway ORF clone of human RPS6KA1 [NM_002953.3] with stop codon (for native or N-terminal fusions)DepositorInsertRPS6KA1 (RPS6KA1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E290 Hs.RPS6KA1-nostop
Plasmid#70574PurposeGateway ORF clone of human RPS6KA1 [NM_002953.3] without stop codon (for C-terminal fusions)DepositorInsertRPS6KA1 (RPS6KA1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pFLAG-CMV-hErk1
Plasmid#49328PurposeExpresses FLAG-tagged human Erk1DepositorInsertErk1 (MAPK3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKH3-human RSK1
Plasmid#13841DepositorInsertRSK1 (RPS6KA1 Human)
UseTags3x HAExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag p38 delta
Plasmid#20785DepositorInsertpDCNA3 Flag p38 delta (Mapk13 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -