Purpose
3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
Insert
3XHA_den1FnCas9_KRAB_eGFP
Use
CRISPR
Tags
3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP
Expression
Mammalian
Mutation
Changed Glutamic Acid to Arginine at 1369 position
Available since
Sept. 11, 2023
Availability
Academic Institutions and Nonprofits only