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Plasmid#178857PurposeMammalian expression of PKCδ-eDHFR(69K6) chimera fused to EGFPDepositorInsertPKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-EGFP (PRKCD Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-chPKCdelta/eDHFR(69K6)-miRFP670
Plasmid#178858PurposeMammalian expression of PKCδ-eDHFR(69K6) chimera fused to miRFP670DepositorInsertPKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-miRFP670 (PRKCD Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PRPS1L1 gRNA (BRDN0001145013)
Plasmid#75838Purpose3rd generation lentiviral gRNA plasmid targeting human PRPS1L1DepositorInsertPRPS1L1 (PRPS1L1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRPS1L1 gRNA (BRDN0001146253)
Plasmid#75839Purpose3rd generation lentiviral gRNA plasmid targeting human PRPS1L1DepositorInsertPRPS1L1 (PRPS1L1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRPS1L1 gRNA (BRDN0001146979)
Plasmid#75840Purpose3rd generation lentiviral gRNA plasmid targeting human PRPS1L1DepositorInsertPRPS1L1 (PRPS1L1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRPS1L1 gRNA (BRDN0001147147)
Plasmid#75841Purpose3rd generation lentiviral gRNA plasmid targeting human PRPS1L1DepositorInsertPRPS1L1 (PRPS1L1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHDR_sox2HA_P2A-GFP-pA_HA_G2913A
Plasmid#196191PurposeTagging human SOX2 gene with P2A-EGFP at the C-terminusDepositorInsertSOX2-HAL-P2A-EGFP-SOX2-HAR (SOX2 Human)
UseHomology repair donorTagsExpressionMutationG2913A mutation to replace the guide cutting site…PromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
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TFORF3482
Plasmid#144958PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX2 (SOX2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro
Plasmid#89991PurposeDonor template for generation of SOX2-ntdTomato reporter cell linesDepositorInsertSOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)
UseCRISPRTagsExpressionMutationPromoternoneAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-hSOX2
Plasmid#206388PurposeExpresses human SOX2 in mammalian cells, for lentivirus generation.DepositorInsertSOX2 (SOX2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
SOX2 KO (T2A-H2B-EGFP)
Plasmid#167969PurposeSOX2 CDS replacement with T2A-H2B-EGFPDepositorInsertSRY-Box Transcription Factor 2 (SOX2 Human)
UseCRISPR; Donor templateTagsH2B-EGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-ePOUSKM
Plasmid#206398PurposeExpresses four genes (human ePOU, SOX2, KLF4, c-MYC) in mammalian cells, for lentivirus generation.DepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV-hGFAP-GFP-T2A-SOX2
Plasmid#183908PurposeExpression of GFP and human SOX2 under the human GFAP ABC1D promoterDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC
Plasmid#229545PurposeLentiviral construct to express rtTA and SKM proteinsDepositorInsertrTetR-NLS-VP64-P2A-Sox2-T2A-Klf4-E2A-Myc (SOX2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhEF1aAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP4C 156-233
Plasmid#108257PurposeExpresses C-terminal 156-233 residues of CHMP4C with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-9DepositorInsertCHMP4C residues 156-233 (CHMP4C Human)
UseTagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-SOX2-T2A-miRFP670
Plasmid#187375PurposeExpresses human SOX2 and miRFP670 via T2A linker under control of a CMV promoter.DepositorInsertSOX2 (SOX2 Human)
UseTagsT2A-miRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP4C, residues 156-233, no Cys
Plasmid#180642PurposeBacterial expression for CHMP4C C-terminal tail, residues 156-233. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-14.DepositorInsertCHMP4C (CHMP4C Human)
UseTagsHIS-SUMOExpressionBacterialMutationresidues 316-366PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4C(S191C,I225A,L228A,W231A)
Plasmid#199242PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C,I225A,L228A,W231A)DepositorInsertGB1-His6-TEV-CHMP4C(121-233, S191C, I225A, L228A, W231A) (CHMP4C Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationS191C, I225A, L228A, W231APromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PSG9-COMP5AP-AviTag-9xHis
Plasmid#157388PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertPSG9 (PSG9 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only