We narrowed to 7,375 results for: ef1a

1. PB_TRE_P3-PDGFR_EF1a_iRFP670

   Plasmid

   #186305

   Purpose

   Plasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   P3-PDGFR
   iRFP670
   

   Use

   Tags

   HA, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. c-myc-PT3EF1a

   Plasmid

   #92046

   Purpose

   Expresses c-Myc in mammalian cells

   Depositor

   Xin Chen

   Article

   Liu et al Hepatology. 2017 Mar 30. doi: 10.1002/he

   Insert

   C-MYC (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   July 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Ef1a_Large T-antigen_Ires_Puro

   Plasmid

   #18922

   Depositor

   Linzhao Cheng

   Article

   Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

   Insert

   Large T Antigen (SV40gp6 Simian virus 40)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPB_EF1a_Gag-EIF4E

   Plasmid

   #212657

   Purpose

   piggyBac vector to express an MLV Gag-EIF4E fusion protein

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   MLV Gag (UniProt ID: P03332) (gag MoMLV)
   

   Use

   Piggybac

   Tags

   EIF4E

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPB_EF1a_Gag-EIF4E-TM

   Plasmid

   #212658

   Purpose

   piggyBac vector to express an MLV Gag-EIF4E fusion protein (W56L, W102L, W166L mutations installed into EIF4E)

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   MLV Gag (UniProt ID: P03332) (gag MoMLV)
   

   Use

   Piggybac

   Tags

   EIF4E (W56L, W102L, W166L)

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEF1a-mMLH1dn

   Plasmid

   #174825

   Purpose

   Mammalian expression of mouse MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   mouse MLH1dn (codon optimized)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of residues 758-760

   Promoter

   EF1a

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDV05.0_pEF1a-iRFP720

   Plasmid

   #200407

   Purpose

   Expresses iRFP720 constitutively in mammalian cells

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   iRFP720
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLX-EF1a_Empty

   Plasmid

   #221480

   Purpose

   Empty vector control for pLX307 overexpresion experiments

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKLV2-EF1adCas9VP64T2ABsd-W

   Plasmid

   #112919

   Purpose

   Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   dCas9VP64-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1a promoter

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEF1a_CD WT TET1

    Plasmid

    #124082

    Purpose

    Expression of wild-type catalytic domain only form of TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 catalytic domain (TET1 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 alpha

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDV05.2_pEF1a-iRFP720_secreted

    Plasmid

    #200409

    Purpose

    Expresses a secreted version of iRFP720 constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720 (secreted)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDV05.1_pEF1a-iRFP720_frameshifted

    Plasmid

    #200408

    Purpose

    Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720 (frameshift mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLX-EF1a_GFP

    Plasmid

    #221481

    Purpose

    GFP lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHR_EF1a-->GssmycLaG16LaG2_P2A_NLSIFP

    Plasmid

    #162228

    Purpose

    Lentiviral vector for the expression of Myc GFP nanbody LaG16LaG2 with IFP NLS in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    LaG16LaG2 and IFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT3-EF1aH

    Plasmid

    #180149

    Purpose

    This plasmid is an empty vector used as the control for other plasmids which are in pT3-EF1aH backbone. It does not have any restriction site for cloning.

    Depositor

    Xin Chen

    Article

    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB-EF1a-TetOn3G

    Plasmid

    #104543

    Purpose

    PiggyBac vector encoding Tet-On 3G constitutively expressed by EF1a promoter

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    Tet-On 3G
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEF1a-nSpCas9

    Plasmid

    #171691

    Purpose

    Expresses nSpCas9 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    nSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    EF1a

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLX-EF1a_GFP_CdTag-HA

    Plasmid

    #221492

    Purpose

    GFP lentiviral overexpression of C-terminal dTag fusion

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    FKBP F36V

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLX-EF1a_mCherry

    Plasmid

    #221482

    Purpose

    mCherry lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV.EF1a.ChR2-YFP.WPRE.hGH

    Plasmid

    #100056

    Purpose

    AAV expression of ChR2 fused to YFP driven byEF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Insert

    ChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge