We narrowed to 7,508 results for: ef1a

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pT3-EF1aH

Plasmid

#180149

Purpose

This plasmid is an empty vector used as the control for other plasmids which are in pT3-EF1aH backbone. It does not have any restriction site for cloning.

Depositor

Xin Chen

Article

Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

Type

Empty backbone

Expression

Mammalian

Promoter

EF1α

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_Empty

Plasmid

#221480

Purpose

Empty vector control for pLX307 overexpresion experiments

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->HA-mtLaG2

Plasmid

#162225

Purpose

Lentiviral vector for the expression of HA GFP nanobody Lag2 in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-GFP nanobody LaG2

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1amCherry-W

Plasmid

#159295

Purpose

Lentiviral vector expressing mCherry driven by human EF1a promoter

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

human EF1a promoter

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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PB-hEF1A-MG-U6-sgRNA

Plasmid

#175505

Purpose

All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hEF1a, U6

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_GFP_CdTag-HA

Plasmid

#221492

Purpose

GFP lentiviral overexpression of C-terminal dTag fusion

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

EGFP

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1aGFP-W

Plasmid

#159291

Purpose

Lentiviral vector expressing GFP driven by human EF1a promoter

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

human EF1a promoter

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_HA-tNGFR_PuroR

Plasmid

#212652

Purpose

piggyBac vector to express HA-tNGFR with puromycin resistance

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

tNGFR

Use

Piggybac

Tags

HA tag

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg30-PDGFR_EF1a_mCherry

Plasmid

#186309

Purpose

Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg30-PDGFR
mCherry

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg61-PDGFR_EF1a_eGFP

Plasmid

#186310

Purpose

Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg61-PDGFR
eGFP

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_GFP

Plasmid

#221481

Purpose

GFP lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

EGFP

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-B5.GS.CAR-3G

Plasmid

#194464

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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PB_TRE_Z18-PDGFR_EF1a_eGFP

Plasmid

#186304

Purpose

Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

Z18-PDGFR
eGFP

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_Z17-PDGFR_EF1a_mCherry

Plasmid

#186303

Purpose

Plasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

Z17-PDGFR
mCherry

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_AP4-PDGFR_EF1a_eBFP2

Plasmid

#186306

Purpose

Plasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

AP4-PDGFR
eBFP2

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg83-PDGFR_EF1a_iRFP670

Plasmid

#186311

Purpose

Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg83-PDGFR
iRFP670

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg88-PDGFR_EF1a_eBFP2

Plasmid

#186312

Purpose

Plasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg88-PDGFR
eBFP2

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_P9-PDGFR_EF1a_mPlum

Plasmid

#186307

Purpose

Plasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

P9-PDGFR
mPlum

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_AP10-PDGFR_EF1a_mOrange

Plasmid

#186308

Purpose

Plasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

AP10-PDGFR
mOrange

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_P3-PDGFR_EF1a_iRFP670

Plasmid

#186305

Purpose

Plasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

P3-PDGFR
iRFP670

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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