We narrowed to 3,800 results for: pcas9

1. pX330-NL-DHFR-SpCas9

   Plasmid

   #124522

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NL-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX330_Actb sgRNA / hSpCas9

   Plasmid

   #172832

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpCas9(BB)-2A-GFP-G3BP2(1)

   Plasmid

   #136060

   Purpose

   G3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP2 (G3BP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCas9-beta-mmr

   Plasmid

   #169241

   Purpose

   Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Inserts

   cas9
   beta
   mutL-E36K
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Changed glutamic acid 36 to lysine

   Promoter

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAS9-mCherry-ACTG1

   Plasmid

   #66943

   Purpose

   CRISPaint target selector ACTG1

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   gRNA ACTG1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX330_ACTB-i4 sgRNA / hSpCas9

   Plasmid

   #172828

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BII-gR-BtW-eSpCas9

   Plasmid

   #133358

   Purpose

   Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Use

   Tags

   Flag-tagged eSpCas9

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. G1397 DddAtox-C–dSpCas9

   Plasmid

   #157836

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330-Flag-dSpCas9

   Plasmid

   #92113

   Purpose

   Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Cbh

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCas9–mKate2ps–CgRNA

    Plasmid

    #64955

    Purpose

    Control gRNA (GTCAAGGCACTCTTGCCTA)

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAS9-mCherry-HIST1H4C

    Plasmid

    #66944

    Purpose

    CRISPaint target selector HIST1H4C

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA HIST1H4C
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. eSpCas9-EF-Gsk3b(new)

    Plasmid

    #122341

    Purpose

    Expresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Gsk3b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCas9–mKate2ps–T1gRNA

    Plasmid

    #62717

    Purpose

    t1 gRNA (ATGAGAATCAAGGCGGTCGA)

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. eSpCas9(1.1)_No_FLAG_ATP1A1_G2

    Plasmid

    #86610

    Purpose

    Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via nhej using ouabain

    Tags

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

    Plasmid

    #86611

    Purpose

    Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Tags

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSpCas9(BB)-2A-GFP-G3BP2(2)

    Plasmid

    #136061

    Purpose

    G3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP2 (G3BP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX330_ACTB-i1 sgRNA / hSpCas9

    Plasmid

    #172825

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

    Plasmid

    #107727

    Purpose

    Knock out of EB1 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PX458-3xHA-SpCas9

    Plasmid

    #130968

    Purpose

    3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    3xHA-NLS-SpCas9
    

    Use

    CRISPR

    Tags

    3x HA, EGFP, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. eSpCas9(1.1)_No_FLAG_ATP1A1-G7

    Plasmid

    #173203

    Purpose

    Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge