Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 24,396 results for: p

Showing: 2181 - 2200 of 24396 results

  1. pDEST-N2H-C1

    Plasmid
    #125549
    Purpose
    Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment1 attached to c-terminus of Gatew…
    Expression
    Mammalian and Yeast
    Mutation
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125549-plasmid-map-sequence-id-254837

    Enlarge

  2. pDEST-N2H-C2

    Plasmid
    #125559
    Purpose
    Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 attached to c-terminus of Gatew…
    Expression
    Mammalian and Yeast
    Mutation
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125559-plasmid-map-sequence-id-254701

    Enlarge

  3. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

    Enlarge

  4. pDEST-N2H-N2

    Plasmid
    #125548
    Purpose
    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 attached to n-terminus of Gatew…
    Expression
    Mammalian and Yeast
    Mutation
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125548-plasmid-map-sequence-id-254836

    Enlarge

  7. pDONR223 SARS-CoV-2 NSP6_nostop

    Plasmid
    #149309
    Purpose
    Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149309-plasmid-map-sequence-id-296438

    Enlarge

  12. pDONR223 SARS-CoV-2 NSP5_nostop

    Plasmid
    #149308
    Purpose
    Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149308-plasmid-map-sequence-id-296437

    Enlarge

  13. pCjedCas9

    Plasmid
    #172216
    Purpose
    Expresses catalytically inactive Campylobacter jejuni Cas9 (CjedCas9) in bacterial cells
    Depositor
    John van der Oost
    Article
    Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi
    Insert
    catalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
    Use
    Tags
    Histag
    Expression
    Mutation
    changed Asp 8 to Ala and His 559 to Ala
    Promoter
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    172216-plasmid-map-sequence-id-338133

    Enlarge

  15. pDONR207 SARS-CoV-2 E_nostop

    Plasmid
    #149321
    Purpose
    Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149321-plasmid-map-sequence-id-296470

    Enlarge

  16. pGGG-AH-VRT-A2

    Plasmid
    #163703
    Purpose
    Wheat transformation vector pGoldenGreenGate (pGGG) with OsActinP:: hygromycin (hpt) selection and the full native Triticum polonicum VRT-A2 gene (native prom::genomic seq::3'UTR)
    Depositor
    Cristobal Uauy
    Article
    Adamski et al Plant Cell. 2021 Aug 13;33(7):2296-2319.
    Inserts
    Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
    Triticum polonicum VRT-A2 genomic sequence (Native promoter:: genomic sequence::3'UTR) + Nos Terminator
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Rice - Os Actin promoter and Triticum polonicum V…
    Available since
    Jan. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    163703-plasmid-map-sequence-id-320920

    Enlarge

  18. pDONR207 SARS-CoV-2 NSP1_nostop

    Plasmid
    #149304
    Purpose
    Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    May 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149304-plasmid-map-sequence-id-296393

    Enlarge

  19. pYeDP60-hATP13A2-E343A

    Plasmid
    #171378
    Purpose
    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888
    Depositor
    Peter Vangheluwe
    Article
    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:
    Insert
    human ATP13A2 variant 2 wild-type
    Use
    Tags
    BAD tag
    Expression
    Yeast
    Mutation
    E343A
    Promoter
    URA3
    Available since
    Sept. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171378-plasmid-map-sequence-id-342930

    Enlarge

Showing: 2181 - 2200 of 24396 results