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HRV-B3 IRES

Plasmid

#189969

Purpose

Part 2 plasmid to introduce the HRV-B3 IRES into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

HRV-B3 IRES

Use

Cloning

Promoter

N/A

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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CVB3 IRES

Plasmid

#189968

Purpose

Part 2 plasmid to introduce the CVB3 IRES into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

CVB3 IRES

Use

Cloning

Promoter

N/A

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pRK-Myc-Smurf1

Plasmid

#13676

Depositor

Ying Zhang

Article

Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

Insert

Smurf1 (SMURF1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_PAsfCherry2(1-10)

Plasmid

#82612

Purpose

Express PAsfCherry2(1-10) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

PAsfCherry2(1-10)

Expression

Mammalian

Mutation

changed Glu 118 to Gln, changed Thr 128 to Ile

Promoter

CMV

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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M-SPOTIT1.1

Plasmid

#171216

Purpose

Expresses a mu-opioid receptor-based circular permuted green fluorescent protein sensor

Depositor

Wenjing Wang

Article

Kroning et al Angew Chem Int Ed Engl. 2021 Mar 4. doi:

Insert

M-SPOTIT1.1

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHCMV_DUGV_GP

Plasmid

#207268

Purpose

Mammalian expression of Dugbe virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DUGV_GP

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET24b-BR1.4

Plasmid

#199436

Purpose

expresses BAmRed1.4 in bacterial cells

Depositor

Andrew Woolley

Article

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

BAMRed1.4

Tags

his tag

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_FLUCV_HE

Plasmid

#207275

Purpose

Mammalian expression of Influenza C virus HE protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

FLUCV_HE

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_PUUV_GP

Plasmid

#207301

Purpose

Mammalian expression of Puumala virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

PUUV_GP

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET24b-BG1.3

Plasmid

#199437

Purpose

expresses BAmGreen1.3 in bacterial cells

Depositor

Andrew Woolley

Article

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

BAMGreen1.3

Tags

his tag

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_DHOV_GP

Plasmid

#207267

Purpose

Mammalian expression of Dhori virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DHOV_GP

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_ISFV_G

Plasmid

#207282

Purpose

Mammalian expression of Isfahan virus G protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

ISFV_G

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wmCardinal

Plasmid

#197251

Purpose

Expresses the protein of wmCardinal in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmCardinal

Expression

Worm

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_HCV_E2

Plasmid

#207280

Purpose

Mammalian expression of Hepatitis C virus E2 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

HCV_E2

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_HCV_E1

Plasmid

#207278

Purpose

Mammalian expression of Hepatitis C virus E1 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

HCV_E1

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_rHCaMP

Plasmid

#187107

Purpose

Expression of rHCaMP in mammalian cells

Depositor

Kai Johnsson

Article

Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

Insert

rHCaMP

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pKJ0377

Plasmid

#228712

Purpose

Mammalian expression of human codon-optimized enhanced Bxb1 (epBxb1 mutant T166R)

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

Bxb1_T166R

Expression

Mammalian

Mutation

T166R

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pHCMV_HTNV_GP

Plasmid

#207281

Purpose

Mammalian expression of Hantaan virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

HTNV_GP

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24b-BR1.0

Plasmid

#199435

Purpose

expresses BAmRed1.0 in bacterial cells

Depositor

Andrew Woolley

Article

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

BAMRed

Tags

his tag

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_DUVV_G

Plasmid

#207269

Purpose

Mammalian expression of Duvenhage virus G protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DUVV_G

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JBEI-12050

Plasmid

#84982

Purpose

Isopentenol production

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Insert

MevTo-T21-MK-aphA

Expression

Bacterial

Promoter

LacUV5

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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HEK3_pegF_60bp

Plasmid

#174856

Purpose

Express forward pegRNA mediating 991-bp deletion and 60-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegF_60bp insertion

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HEK3_pegR_60bp

Plasmid

#174857

Purpose

Express reverse pegRNA mediating 991-bp deletion and 60-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegR_60bp insertion

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCBLggPro

Plasmid

#199736

Purpose

Golden Gate entry vector for promoter cloning

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Type

Empty backbone

Expression

Plant

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLggCDS

Plasmid

#199737

Purpose

Golden Gate entry vector for gene of interest cloning

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Type

Empty backbone

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBLggTerm

Plasmid

#199738

Purpose

Golden Gate entry vector for terminator cloning

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Type

Empty backbone

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Rab4 CA

Plasmid

#53466

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab04

Tags

YFP

Expression

Insect

Mutation

Q67L

Promoter

GAL4

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
PJ14

Plasmid

#25912

Depositor

Kenneth Marcu

Article

Lang et al Nucleic Acids Res. 1982 Jan 22. 10(2):61

Insert

Ig germline mu switch region

Expression

Bacterial

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only
ninaE-stops myc

Plasmid

#26856

Depositor

Craig Montell

Article

Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

Insert

Slow Termination of Phototransduction (stops Fly)

Tags

myc

Expression

Insect

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
TwinStrepSUMO-DARPin-SpyCatcher

Plasmid

#243754

Purpose

For affinity purification of E01 anti-EGFR DARPin fused to SpyCatcher

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

DARPin-SpyCatcher

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TwinStrepSUMO-Nb-SpyCatcher

Plasmid

#243752

Purpose

For affinity purification of anti-mouse MHC-II nanobody (Nb) fused to SpyCatcher

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

Nb-SpyCatcher

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TwinStrepSUMO-scFv-SpyCatcher

Plasmid

#243753

Purpose

For affinity purification of anti-HA scFv fused to SpyCatcher

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

scFv-SpyCatcher

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWP78-PVCpnf_pvc13-SNAP

Plasmid

#243741

Purpose

PVC structural operon - tail fiber (Pvc13) tagged with SNAP tag

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-SNAP

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWP78-PVCpnf_pvc13-SpyTag

Plasmid

#243739

Purpose

PVC structural operon - tail fiber (Pvc13) tagged with SpyTag

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-SpyTag

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-eBRIC

Plasmid

#241801

Purpose

Bioluminescence indicator for Ca2+ imaging in neurons

Depositor

Huiwang Ai

Article

Tian et al ACS Sens. 2025 Jul 29. doi: 10.1021/acss

Inserts

eBRIC
eBRIC

Use

AAV

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
XZ039 CMV-TO-Shc1-MCP (FLP-IN)

Plasmid

#233392

Purpose

Shc1-tdMCP

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

Shc1-tdMCP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKJ247

Plasmid

#228714

Purpose

Mammalian expression of U6 epPsaCas12f RNF2 targeting guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

u6-RNF2 targeting guide

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXWTM-X2S-C

Plasmid

#225967

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of serine in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2S-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXWTM-X2N-C

Plasmid

#225968

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of asparagine in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2N-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXWTM-X2D-C

Plasmid

#225969

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of aspartic acid in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2D-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXWTM-X2E-C

Plasmid

#225970

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of glutamic acid in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2E-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXMCR-TLY-X2A-C

Plasmid

#225971

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGADTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTMCR-TLY-X2A-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXMCR-TLY-X2H-C

Plasmid

#225972

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGHDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2H-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXMCR-TLY-X2S-C

Plasmid

#225973

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGSDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2S-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXMCR-TLY-X2N-C

Plasmid

#225974

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGNDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2N-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXMCR-TLY-X2D-C

Plasmid

#225975

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGDDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2D-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXMCR-TLY-X2E-C

Plasmid

#225976

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGEDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2E-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXWTM-X2A-C

Plasmid

#225965

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of alanine in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2A-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXWTM-X2H-C

Plasmid

#225966

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of histidine in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2H-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF11-wmNeonGreen

Plasmid

#197242

Purpose

Expresses the protein of wNeonGreen in neurons of C elegan

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmNeonGreen

Expression

Worm

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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