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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,305 results for: LEA

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Showing: 2201 - 2220 of 12305 results

  4. pBAD24-sfGFPx2

    Plasmid
    #51559
    Purpose
    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)
    Depositor
    Sankar Adhya, Francisco Malagon
    Publication
    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261
    Insert
    superfolder GFP
    Tags
    GLESTCRHASLAVLADERRFSA and MARARA
    Expression
    Bacterial
    Mutation
    Contains additonal aa in the N and C terminus (in…
    Available since
    March 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51559-plasmid-map-sequence-id-78831

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  6. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

    Plasmid
    #89873
    Purpose
    expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production
    Depositor
    Hyungbae Kwon
    Publication
    Lee et al Nat Methods. 2017 May;14(5):495-503. doi
    Insert
    bArrestin2-TevC-P2A-TdTomato
    Use
    AAV
    Tags
    TdTomato
    Expression
    Mammalian
    Mutation
    R173H in TdTomato (Please see depositor comments …
    Promoter
    Human synapsin
    Available since
    May 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89873-plasmid-map-sequence-id-177108

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  7. pMM178

    Plasmid
    #61270
    Purpose
    Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.
    Depositor
    Timothy Lu
    Publication
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    pLtetO, native, native (respectively)
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61270-plasmid-map-sequence-id-99577

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  12. pcDNA3-Mito-ExRai-AMPKAR

    Plasmid
    #192448
    Purpose
    ExRai-AMPKAR targeted to the mitochondrial outer membrane.
    Depositor
    Jin Zhang
    Publication
    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mito-ExRai-AMPKAR
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192448-plasmid-map-sequence-id-379142

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  16. pNJP

    Plasmid
    #115494
    Purpose
    Expression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cells
    Depositor
    Kazuhiro Aoki
    Publication
    Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-iRFP-NLS-P2A-JNK KTR-mCherry-P2A-mKO-MK2 (MAPK14, MAPK8 Mouse, Synthetic, Human)
    Tags
    iRFP, mCherry, and mKO
    Expression
    Mammalian
    Mutation
    mCherry S227F, mCherry G229R, mKO V211G
    Promoter
    CAG
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    115494-plasmid-map-sequence-id-231521

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  17. NR4A1

    Plasmid
    #25477
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NR4A1 (NR4A1 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  18. pcDNA3-StagRFP

    Plasmid
    #173014
    Purpose
    Rationally enhanced red fluorescent protein derived from TagRFP-T. For mammalian expression.
    Depositor
    Jin Zhang
    Publication
    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    StagRFP
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    173014-plasmid-map-sequence-id-343313

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  19. pNuc-TrxRFP1

    Plasmid
    #98998
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization
    Depositor
    Huiwang Ai
    Publication
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TrxRFP1 and nuclear localization sequence
    Tags
    His-tag and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    98998-plasmid-map-sequence-id-190366

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Showing: 2201 - 2220 of 12305 results