We narrowed to 7,649 results for: Pol

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pPRIME-TET-GIN-recipient

Plasmid

#11656

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP-IRES-Neo expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP, IRES, and Neo

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pLEX307 hDVL1

Plasmid

#102863

Purpose

Expresses human DVL1 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

human Dishevelled 1 (DVL1) (DVL1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed alanine 2 to glycine. Likely due to poly…

Promoter

EF1A

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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Arp2/3_pBIG2abcd

Plasmid

#173683

Purpose

Insect expression of the 7-subunit Human Arp2/3 complex

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Inserts

Arp2
Arp3
ArpC2
ArpC3-FLAG
ArpC4
ArpC5
ArpC1

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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IMPT-10314:GABBR2

Plasmid

#157851

Purpose

HA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expression

Depositor

Vadim Cherezov, Raymond Stevens

Article

Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

Insert

GABBR2 (GABBR2 Human)

Expression

Insect

Promoter

Polyhedrin

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CMVie-IRES-BlastR alt MCS

Plasmid

#120862

Purpose

3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

Depositor

Ghassan Mouneimne

Article

Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV immediate early

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE M33 flag

Plasmid

#1952

Depositor

Robert Kingston

Article

Levine et al Mol Cell Biol 2002 Sep;22(17):6070-8.

Insert

M33 (Cbx2 Mouse)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pCMV6-AN-HA_WT REV1

Plasmid

#201586

Purpose

Expresses human DNA repair protein REV1 with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA repair protein REV1 (REV1 Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-PLtetO1-tetR-T500

Plasmid

#45391

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

tet repressor

Expression

Bacterial

Promoter

PL-tetO1

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pEIAV-SIN6.1 CBGFPW

Plasmid

#44173

Depositor

John Olsen

Insert

enhanced green fluorescent protein

Use

Lentiviral

Mutation

chromophore mutations

Promoter

CB, CMV enhancer/chicken beta-actin promoter

Available Since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM2-Flag

Plasmid

#180407

Purpose

lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM2 (TGM2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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His-GFP-Clathrin

Plasmid

#170858

Purpose

Mammalian expression of His-GFP-Clathrin.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Clathrin light polypeptide (Lca) (Clta Mouse)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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p3XFlag-Mettl4

Plasmid

#86665

Purpose

Expresses murine Mettl4 containing N-terminal 3XFlag tag

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

Mettl4 (Mettl4 Mouse)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pRET.IL.PGK-TK

Plasmid

#1837

Depositor

Philip Leder

Article

Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35

Type

Empty backbone

Use

Cre/Lox and Retroviral

Expression

Mammalian

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
AP-p002

Plasmid

#31493

Depositor

David Wang

Article

Gaynor et al PLoS Pathog. 2007 May 4. 3(5):e64.

Insert

Half genome of WU polyomavirus

Expression

Bacterial

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pET28a-MSP1D1deltaH4/2H5

Plasmid

#71715

Purpose

Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP11 CS puroR

Plasmid

#46748

Purpose

Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP11

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11 (USP11 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

C318S--catalytically inactive

Promoter

CMV

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pIC306

Plasmid

#52257

Purpose

Retroviral expression of EGFP-TEV-S-tag SKA3 for protein localization and affinity purification (LAP)

Depositor

Iain Cheeseman

Article

Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

Insert

Ska3 (SKA3 Human)

Use

Retroviral

Tags

EGFP, S tag, and TEV protease site

Expression

Mammalian

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDWUER

Plasmid

#37094

Depositor

David Wang

Article

Gaynor et al PLoS Pathog. 2007 May 4. 3(5):e64.

Insert

WU polyomavirus early region (WUPyV_gp4, WUPyV_gp5 WU polyomavirus)

Expression

Mammalian

Promoter

CMV

Available Since

July 3, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-TRE-C-prM-E-NS1

Plasmid

#175277

Purpose

AAV vector mediating inducible expression of 4 genes (the C-prM-E-NS1) of YFV-17D

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

C-prM-E-NS1 (POLY Yellow fever virus strain 17D)

Use

AAV

Promoter

tight TRE

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Myo1E-pEGFP

Plasmid

#124658

Purpose

Mouse myosin-1e fused with EGFP

Depositor

Cheng-han Yu

Article

Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

Insert

Myo1e (Myo1e Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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