We narrowed to 15,989 results for: LEA

1. pFastBac-HS-NLRC4Δcard

   Plasmid

   #177276

   Purpose

   Purification of mouse NLRC4 (90-1024) from insect cells

   Depositor

   Hao Wu

   Article

   Zhang et al Science. 2015 Oct 23;350(6259):404-9. do

   Insert

   NLRC4 (Nlrc4 Mouse)
   

   Tags

   HIS-SUMO-PreScission

   Expression

   Insect

   Mutation

   I586T, N761S, M885V, K915R and F972L- please see …

   Promoter

   polyhedrin

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCLASP_Crz1(19A)_CLASP_RGS2membrane

   Plasmid

   #133085

   Purpose

   Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   Crz1*-CLASP
   

   Expression

   Bacterial and Yeast

   Promoter

   pADH1

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NET37 pmRFP-N2 (450)

   Plasmid

   #61980

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

   Insert

   NET37 (KIAA1161 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pTE3987

   Plasmid

   #137716

   Purpose

   Expresses dLbCas12a-RVRR-BE in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   dLbCas12a-RVRR-BE
   

   Tags

   6xHis, APOBEC-1, SV40 NLS, and UGI

   Expression

   Mammalian

   Mutation

   G532A, K538V, Y542R, K595R

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-EF1a-NES-tdTomato-LINuS-WPRE

   Plasmid

   #159894

   Purpose

   AAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signal

   Depositor

   Tatsushi Onaka

   Article

   Inutsuka et al Biochem Biophys Res Commun. 2020 Jan 29;

   Insert

   NES-tdTomato-LINuS
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1α promoter

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-JNKsub(TA)DD-YPet-NES

   Plasmid

   #84634

   Purpose

   Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   JNKsub(TA)DD-YPet-NES
   

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TAPBPL pEGFP-C3 (1765)

   Plasmid

   #62055

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

   Insert

   TAPBPL (TAPBPL Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

8. Hy_pMT Flag-UAP56 MUT

   Plasmid

   #110124

   Purpose

   Expresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   UAP56 (DDX39B Human)
   

   Tags

   Flag

   Expression

   Insect

   Mutation

   4 mutations (KSLN motif changed to RSFS)

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ADAP1

   Plasmid

   #163905

   Purpose

   Full length mouse ADAP1 for purification from insect cells

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   ADAP1 (Adap1 Mouse)
   

   Tags

   Cleavable 6 x HIS tag

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-4T3-ZO-2-1st-PDZ-delta-NLS

    Plasmid

    #27425

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Deletion of NLS-Nuclear Localization Signal: Nucl…

    Available Since

    May 18, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pCS2 Notch1 LNG (CC→SS)-6MT

    Plasmid

    #41739

    Depositor

    Raphael Kopan

    Article

    Mumm et al Mol Cell. 2000 Feb;5(2):197-206.

    Insert

    Notch-1 Lin-Notch-glp (LNG) repeat-containing construct, CC->SS (Notch1 Mouse)
    

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Contains murine Notch-1 leader peptide (aa 1-23) …

    Available Since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

12. TTR F87E

    Plasmid

    #238075

    Purpose

    Bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Leach et al J Mol Biol. 2024 Aug 15;436(16):168673.

    Insert

    TTR (TTR Human)
    

    Expression

    Bacterial

    Mutation

    F87E

    Promoter

    T7

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHAGE2-TetO-Chim-Pchi-Q61A

    Plasmid

    #231520

    Purpose

    Expresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generation

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Pchi HMG Q61A flanked with N- and C-termini of mSox2
    

    Use

    Lentiviral

    Mutation

    Point mutation at position 61 Q to A of the HMG d…

    Available Since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHAGE2-TetO-Chim-Pchi-Q61A/E64M

    Plasmid

    #231521

    Purpose

    Expresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generation

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Pchi HMG Q61A/E64M flanked with N- and C-termini of mSox2
    

    Use

    Lentiviral

    Mutation

    Point mutations at position 61 Q to A and 64 E to…

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMBS-845

    Plasmid

    #218000

    Purpose

    Strep-Tag II fused to PreScission Protease cleavage site for protein purification and cleavage

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    Strep-tag II
    

    Use

    Synthetic Biology

    Tags

    PreScission protease cleavage site

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMBS-846

    Plasmid

    #218001

    Purpose

    3xFLAG-tag fused to PreScission Protease cleavage site for protein purification and cleavage

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    3xFLAG-tag
    

    Use

    Synthetic Biology

    Tags

    PreScission protease cleavage site

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMBS-847

    Plasmid

    #218002

    Purpose

    Myc-tag fused to PreScission Protease cleavage site for protein purification and cleavage

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    Myc-tag
    

    Use

    Synthetic Biology

    Tags

    PreScission protease cleavage site

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

    Plasmid

    #218916

    Purpose

    Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
    

    Use

    Lentiviral

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. KIF13B

    Plasmid

    #163907

    Purpose

    Full length mouse KIF13B for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    kif13b (Kif13b Mouse)
    

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KIF13B 1-584

    Plasmid

    #163908

    Purpose

    Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    kif13b (Kif13b Mouse)
    

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge