We narrowed to 2,477 results for: T2A

1. pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

   Plasmid

   #181975

   Purpose

   Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   FMC6.3-BBz CAR; tNGFR (NGFR Human, Synthetic)
   

   Use

   Lentiviral

   Mutation

   NGFR truncation: aa 11-276

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

   Plasmid

   #181974

   Purpose

   Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   FMC6.3-28z CAR; tNGFR (NGFR Human, Synthetic)
   

   Use

   Lentiviral

   Mutation

   NGFR truncation: aa 11-276

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV_U6-ωRNA*_ISDra2-TnpB-mNG

   Plasmid

   #212968

   Purpose

   AAV vector for encoding an ISDra2-TnpB driven by EFs promoter

   Depositor

   Chunlong Xu

   Article

   Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

   Inserts

   ISDra2-TnpB-T2A-mNG,
   ωRNA*
   

   Use

   AAV and CRISPR

   Tags

   NLS and NLS-FLAG

   Expression

   Mammalian

   Promoter

   EF1a, T7 and U6

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. ISDra2-TnpB-mCherry-U6-ωRNA*

   Plasmid

   #212967

   Purpose

   Expression vector for expression of ISDra2-TnpB driven by chicken β-actin promoter and mCherry driven by CMV promoter

   Depositor

   Chunlong Xu

   Article

   Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

   Inserts

   ISDra2-TnpB-T2A-mCherry
   ωRNA*
   

   Use

   CRISPR

   Tags

   NLS and NLS-FLAG

   Expression

   Mammalian

   Promoter

   CAG, T7 and U6

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

   Plasmid

   #132393

   Purpose

   Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   human TauP301L 2N4R-EGFP (MAPT Human, Synthetic)
   

   Use

   CRISPR and Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Proline 301 to Leucine (P301L)

   Promoter

   TREtight

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE2-TetOminiCMV-OSK

   Plasmid

   #136552

   Purpose

   Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4-P2A-Sox2-T2A-Klf4 (Klf4, Sox2, Pou5f1 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial

   Promoter

   tetO-miniCMV (dox-inducible)

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MIP 339 CoMiP coLin28 2A Nanog IRES eGFP

   Plasmid

   #63729

   Purpose

   To express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

   Depositor

   Joseph Wu, Mark Kay

   Article

   Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

   Inserts

   Lin28 (LIN28A Human)
   Nanog (NANOG Human)
   

   Tags

   T2A

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   SFFV

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPaint-mScarlet-PuroR [M1G]

   Plasmid

   #171809

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-T2A-PuroR
   

   Use

   Gene tagging

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPaint-mScarlet-BlastR [M1G]

   Plasmid

   #171814

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-T2A-BlastR
   

   Use

   Gene tagging

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE2-TetOminiCMV-SKM

    Plasmid

    #136551

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Sox2, Klf4, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2-T2A-Klf4-E2A-cMyc (Sox2, Klf4, Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPaint-mNeon-PuroR [M1G]

    Plasmid

    #171800

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPaint-mNeon-BlastR [M1G]

    Plasmid

    #171805

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-BlastR
    

    Use

    Gene tagging

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUW-tetO-hOKMminiSOX

    Plasmid

    #206383

    Purpose

    Expresses four genes (human OCT4, KLF4, c-MYC and mouse miniSOX) in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

    Insert

    OCT4, KLF4, c-MYC, miniSOX (POU5F1, KLF4, MYC Human, Mouse)
    

    Use

    Lentiviral

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE2-TetOminiCMV-OKM

    Plasmid

    #136554

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Klf4, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-T2A-Klf4-E2A-cMyc (Pou5f1, Myc, Klf4 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. rTTA-gRNA-AAV

    Plasmid

    #213036

    Purpose

    This vector contains rTTA expressed under CMV promoter and gRNA expressed under U6 promoter

    Depositor

    Nadav Ahituv

    Article

    Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

    Insert

    rTTA-T2A-mCherry
    

    Use

    AAV and CRISPR

    Promoter

    CMV

    Available Since

    Oct. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB-TR-hCAII

    Plasmid

    #232480

    Purpose

    Tetracycline inducible PiggyBac vector expressing human CAII gene including flag-tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Human carbonic anhydrase II (CA2 Human)
    

    Use

    Transposon system

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Promoter

    CMV-TetO

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-K36M TAIL[1-44]-3AID-HA-2A-mCherryBSD

    Plasmid

    #225872

    Purpose

    Lentiviral expression of AID degron tagged H3.3(K36M) histone tail corresponding to amino acids 1-44 and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3(K36M) amino acids 1-44 (H3-3A, H3-3B Human, Synthetic)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mCherry

    Expression

    Mammalian

    Mutation

    amino acids 1-44 from H3.3 K36M

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-GCN5

    Plasmid

    #65386

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    GCN5 (KAT2A Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF2959

    Plasmid

    #144435

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KAT2A (KAT2A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-KIF1B beta-FL

    Plasmid

    #79108

    Purpose

    Mammalian expression of GFP-KIF1B beta-FL

    Depositor

    Susanne Schlisio

    Article

    Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

    Insert

    GFP-KIF1B beta full length (KIF1B Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    exon 25 missing (natural splice varient)

    Promoter

    CMV

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only