We narrowed to 36,941 results for: kan

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pHCKan-yibDp-mCherry

Plasmid

#127058

Purpose

Low phosphate inducible mCherry with N-terminal linker and His tag

Depositor

Michael Lynch

Publication

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

mCherry

Expression

Bacterial

Promoter

yibDp

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDonor14-AcHELIX-KanR (CJT96)

Plasmid

#181794

Purpose

pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt-3HA

Plasmid

#39284

Depositor

Jurg Bahler, John Pringle

Publication

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Mutation

-1163 to +6 of nmt1 locus with a 4bp deletion (TA…

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pMST636_BB3_L_AD_hyg_KanOri_pyrG5

Plasmid

#90281

Purpose

BB3 recipient for homologous integration in pyrG locus with linker AD

Depositor

Michael Sauer

Publication

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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GPKAnes

Plasmid

#61092

Purpose

GFP-tagged PKA catalytic subunit targeted to the extranuclear cytoplasm by addition of a nuclear export signal

Depositor

Steven Green

Publication

Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.

Insert

pkac2

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pUC57KAN-80XALFA

Plasmid

#233124

Purpose

80X ALFA tag multimer

Depositor

Steve Stowers

Insert

ALFA tag

Use

Unspecified

Mutation

None

Available since

March 31, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57KAN-20XALFA

Plasmid

#233112

Purpose

20X ALFA tag multimer

Depositor

Steve Stowers

Insert

ALFA tag

Use

Unspecified

Mutation

None

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pKanB alpha

Plasmid

#223524

Purpose

Used for expression of secreted recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

Depositor

Geoff Lin-Cereghino

Publication

Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

Type

Empty backbone

Expression

Yeast

Promoter

AOX1

Available since

Sept. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pgRNAkan-glpR

Plasmid

#169873

Purpose

Single guide RNA plasmid for Cas9 targeting glpR in P. putida

Depositor

Brian Pfleger

Publication

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

sgRNA towards glpR in Pseudomonas putida

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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RS02060_pMQ30_KO_Kan

Plasmid

#185391

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS02060 in C. basilensis 4G11

Depositor

Adam Arkin

Publication

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS02060 upstream flanking homology region
RR42_RS02060 downstream flanking homology region

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS28935_pMQ30_KO_Kan

Plasmid

#185392

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS28935 in C. basilensis 4G11

Depositor

Adam Arkin

Publication

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS28935 upstream flanking homology region
RR42_RS28935 downstream flanking homology region

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS18965_pMQ30_KO_Kan

Plasmid

#185394

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS18965 in C. basilensis 4G11

Depositor

Adam Arkin

Publication

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS18965 upstream flanking homology region
RR42_RS18965 downstream flanking homology region

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS18970_pMQ30_KO_Kan

Plasmid

#185395

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS18970 in C. basilensis 4G11

Depositor

Adam Arkin

Publication

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS18970 upstream flanking homology region
RR42_RS18970 downstream flanking homology region

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS17060_pMQ30_KO_Kan

Plasmid

#185396

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS17060 in C. basilensis 4G11

Depositor

Adam Arkin

Publication

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS17060 upstream flanking homology region
RR42_RS17060 downstream flanking homology region

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS17055_pMQ30_KO_Kan

Plasmid

#185398

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11

Depositor

Adam Arkin

Publication

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS17055 upstream flanking homology region
RR42_RS17055 downstream flanking homology region

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDZ645 pKAN 1x-mCherry-12xPP7 V4

Plasmid

#73173

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

Depositor

Daniel Zenklusen

Publication

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

1x-mCherry

Expression

Mammalian and Yeast

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHCKan-yibDp2-GFPuv

Plasmid

#139083

Purpose

Low phosphate inducible promoter yibDp2 for GFP expression

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

yibDp2-GFPuv

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKD4_mCherry

Plasmid

#187386

Purpose

To chromosomally tag genes of interest with mCherry. FRT-mCherry-kan-FRT

Depositor

Roi Avraham

Insert

mCherry

Expression

Bacterial

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pUC-H1-Esp3I-mU6-Kan

Plasmid

#213015

Purpose

Vector for Cloning of multiple gRNAs driven by distinct promoters (H1 and mU6)

Depositor

Stephanie Roessler

Insert

H1 promoter, mU6 promoter, gRNA scaffolds

Promoter

H1 promoter

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCY5.1kh

Plasmid

#160297

Purpose

Yeast CRISPR plasmid targeting the kanMX and hphMX cassettes

Depositor

Amir Aharoni

Publication

Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

Inserts

sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
URA3MX selection cassette
sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
Cas9

Use

CRISPR

Tags

8xHis-tag and SV40 NLS

Available since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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