We narrowed to 24,295 results for: Spr;

1. hgRNA-A76_pLKO-Hyg

   Plasmid

   #100568

   Purpose

   hgRNA-A76 in a lentiviral backbone

   Depositor

   George Church

   Article

   Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

   Insert

   hgRNA-A76
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. hgRNA-B21_pLKO-Hyg

   Plasmid

   #100560

   Purpose

   hgRNA-B21 in a lentiviral backbone

   Depositor

   George Church

   Article

   Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

   Insert

   hgRNA-B21
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGRHL2.1.0-gDNA

   Plasmid

   #175863

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   GRHL2 (GRHL2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. U6>Ebf.774(F+E)

   Plasmid

   #59990

   Purpose

   U6 promoter driving Ebf.774 (F+E) sgRNA

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   Ebf.774 (F+E) sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ciinte.U6

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMZ285

   Plasmid

   #52226

   Purpose

   Expression of sgRNA precursor targeted against ade6M210

   Depositor

   Mikel Zaratiegui

   Article

   Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

   Insert

   rrk1-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   rrk1

   Available since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pb-CAGGS-dCas9-VP64-blasto

   Plasmid

   #218556

   Purpose

   Encodes CAG promoter-driven dCas9-VP64 with a bipartite SV40 NLS between dCas9 and VP64, along with blasticidin resistance

   Depositor

   Alejandro Chavez

   Insert

   dCas9
   

   Use

   CRISPR; Piggybac transposition

   Tags

   2xSV40NLS-VP64

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   Available since

   Aug. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. (829) SB Tet-ON dCasphi-[myc-ZIM3-HA] U6-gStop

   Plasmid

   #195622

   Purpose

   Sleeping Beauty Tet-ON plasmid for induced expression of dCasphi-ZIM3 CRISPRi construct

   Depositor

   Tonia Rex

   Insert

   dCasphi
   

   Use

   Tags

   C-Myc (NLS), HA tag, and ZIM3

   Expression

   Mammalian

   Mutation

   P749A and P753A

   Promoter

   Tight TRE promoter; Tet-ON (doxycycline)

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. (942) pAAV EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

   Plasmid

   #195624

   Purpose

   pAAV EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct

   Depositor

   Tonia Rex

   Insert

   dCasphi
   

   Use

   AAV

   Tags

   C-Myc (NLS), HA tag, and ZIM3

   Expression

   Mammalian

   Mutation

   P749A and P753A

   Promoter

   EF1a (Mlu1/EcoR1)

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSG1017-2xBsaI-SaKKH-2xUGI_P2A_EGFP

   Plasmid

   #239450

   Purpose

   Codon-optimized S. aureus KKH Cas9 with 2x UGI. 2x BsaI sites for easy cloning of varying deaminases for cytosine base editing. EGFP can serve as a transfection marker.

   Depositor

   Alexis Komor

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSG1039-rA1-SaKKH_P2A_EGFP

    Plasmid

    #239462

    Purpose

    Cytosine base editor with codon-optimized S. aureus KKH Cas9 and rAPOBEC1 deaminase. EGFP can be used as a transfection marker.

    Depositor

    Alexis Komor

    Insert

    S.a. rA1 CBE
    

    Use

    CRISPR

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pARID4B.1.0-gDNA

    Plasmid

    #132436

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ARID4B (ARID4B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHMG20B.1.0-gDNA

    Plasmid

    #132449

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    HMG20B (HMG20B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBRCA2.1.0-gDNA

    Plasmid

    #132448

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    BRCA2 (BRCA2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMECOM.1.0-gDNA

    Plasmid

    #132445

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    MECOM (MECOM Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. ReaLiSM A1-43-GPIba+2cys

    Plasmid

    #59577

    Purpose

    express VWF A1-GPIba ReaLiSM construct for single molecule study

    Depositor

    Timothy Springer

    Insert

    VWF A1-linker-GPIba (VWF Human)
    

    Use

    Tags

    6His

    Expression

    Mammalian

    Mutation

    Promoter

    chick beta-actin

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pMZ286

    Plasmid

    #52227

    Purpose

    Expression of sgRNA precursor targeted against ade6L469

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Insert

    rrk1-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    rrk1

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pdCas9-sgRNA-RFP

    Plasmid

    #166005

    Purpose

    Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

    Depositor

    Martin Buck

    Article

    Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFD6

    Plasmid

    #73915

    Purpose

    tissue-specific multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    :tRNA:gRNA:tRNA:gRNA:tRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS-DSCP

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX458-AAVS1

    Plasmid

    #113194

    Purpose

    Encodes gRNA for human AAVS1

    Depositor

    Adam Karpf

    Insert

    AAVS1 (PPP1R12C, AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBGK

    Plasmid

    #65796

    Purpose

    Vector for Cas9 mRNA synthesis

    Depositor

    Masato Ohtsuka

    Article

    Harms et al Curr Protoc Hum Genet. 2014 Oct 1;83:15.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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