We narrowed to 4,744 results for: NAP

1. CpX-1

   Plasmid

   #218676

   Purpose

   Expresses complexin-1 in bacteria

   Depositor

   Axel Brunger

   Article

   Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

   Insert

   Rat complexin-1 (Cplx1 Rat)
   

   Use

   Tags

   6 his

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-FKBP-GFP-OPTN

   Plasmid

   #208866

   Purpose

   Stably express GFP-tagged OPTN for CID induced mitophagy

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   OPTN (OPTN Human)
   

   Use

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

   Plasmid

   #219653

   Purpose

   General mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark Venus

   Depositor

   Thomas Oertner

   Insert

   2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
   

   Use

   AAV

   Tags

   2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)

   Expression

   Mammalian

   Mutation

   Promoter

   nEf Addgene # 149296

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-Duet_OPTN-thrombin-GST

   Plasmid

   #216843

   Purpose

   For expression in E. coli of OPTN (Internal ref. SMC1789)

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   Optineurin (OPTN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETmsGFPSyt1(80 JuxtaK-142)

   Plasmid

   #209013

   Purpose

   Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Use

   Tags

   6xHis-msfGFP

   Expression

   Bacterial

   Mutation

   aa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

   Promoter

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETSyt1C2AB(143-421)msGFP

   Plasmid

   #209010

   Purpose

   Recombinant protein expression of truncated syt1 tagged with GFP

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Use

   Tags

   6xHis and msfGFP

   Expression

   Bacterial

   Mutation

   aa 143-421 only

   Promoter

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETSUMOSyt1C2AB(80 JuxtaK-421)

   Plasmid

   #209008

   Purpose

   Recombinant protein expression of truncated syt1 construct with Juxta K mutations

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

   Promoter

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFSynWSyt1C2AB(143-421)msGFP

   Plasmid

   #209015

   Purpose

   Overexpression of truncated syt1 tagged with GFP in mammalian cells

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   aa 143-421 only

   Promoter

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX SYT9 C2A

   Plasmid

   #195707

   Purpose

   Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   encodes amino acids 124-211 of mouse SYT9

   Promoter

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX SYT9 C2B

    Plasmid

    #195708

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 253-344 of mouse SYT9

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX SYT9 C2AB

    Plasmid

    #195709

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 104-386 of mouse SYT9

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX SYT1 C2A

    Plasmid

    #195710

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT1 C2A domain

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 96-264 of mouse SYT1

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX SYT1 C2B

    Plasmid

    #195711

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT1 C2B domain

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 248-421 of mouse SYT1

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX SYT1 C2AB

    Plasmid

    #195712

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT1 C2AB domain

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 96-421 of mouse SYT1

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET (SUMO)-Syt9

    Plasmid

    #195713

    Purpose

    Bacterial expression plasmid encoding SUMO-tagged full-length Syt9

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Rat)
    

    Use

    Tags

    GST-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNMSB91

    Plasmid

    #199319

    Purpose

    Combine with Gal4 to direct ACR1 expression

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    15xUAS::delta pes-10::::ACR1::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    15xUAS::delta pes-10

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28b_TypeIC_acrIF2_invivo

    Plasmid

    #196402

    Purpose

    expresses acrIF2 protein targeting CRISPR Cascade Type IC for in vivo assay

    Depositor

    David Taylor

    Article

    O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

    Insert

    anti-CRISPR IF2 protein (orf30 P. aeruginosa)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGADT7-AP-1 σ1B

    Plasmid

    #198335

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-1 σ1B
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Expresses a LELQMNRRY nonapeptide after the last …

    Promoter

    ADH1

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGADT7-AP-1 σ1A

    Plasmid

    #198334

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-1 σ1A
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Expresses a LELQMNRRY nonapeptide after the last …

    Promoter

    ADH1

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-hSyn-ASAP3ER

    Plasmid

    #195358

    Purpose

    ASAP3ER under control of human synapsin promoter

    Depositor

    Brenda Bloodgood

    Article

    Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

    Insert

    ASAP3ER
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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