167,453 results

1. pLenti UBC ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)

   Plasmid

   #215012

   Purpose

   Express a ERphagy reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   KDEL
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-ADP-MCS-FLAG

   Plasmid

   #192360

   Purpose

   Adipocyte-specific overexpression of genes

   Depositor

   Yifu Qiu

   Article

   Xue et al Cell Metab. 2022 Sep 6;34(9):1325-1341.e

   Type

   Empty backbone

   Use

   AAV

   Tags

   3xflag

   Expression

   Mammalian

   Promoter

   adiponectin promoter

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICSL11024 (pICH47732::NOSp-NPTII-OCST)

   Plasmid

   #51144

   Purpose

   Confers KAN resistance in planta

   Depositor

   Jonathan D Jones

   Insert

   NOSp-NPTII-OCST
   

   Available Since

   Feb. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHCMV_DENV4_PrME

   Plasmid

   #207266

   Purpose

   Mammalian expression of Dengue virus 4 PrME protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   DENV4_PrME (POLY Dengue virus 4)
   

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS_L_EBOV

   Plasmid

   #103052

   Purpose

   Encodes Ebola virus L gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

   Depositor

   Elke Mühlberger

   Article

   Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

   Insert

   L gene of Ebola virus (L Zaire ebolavirus, Mayinga)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FLEX-tdTomato (AAV5)

   Viral Prep

   #28306-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   tdTomato (Cre-dependent)

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

7. pGL3-U6-sgRNA-PGK-puromycin

   Plasmid

   #51133

   Purpose

   expresses sgRNA from the U6 promoter.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. SMN2E7-Fluc-Nluc-reporter

   Plasmid

   #221005

   Purpose

   Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.

   Depositor

   Mikko Taipale, Benjamin Blencowe

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   Fluc-SMN2E7-STOP-Nluc
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FLEX-tdTomato (AAV Retrograde)

   Viral Prep

   #28306-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. Cre-dependent (FLEX) tdTomato expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   tdTomato (Cre-dependent)

   Available Since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pET28a-gp32-H6

    Plasmid

    #163912

    Purpose

    Expresses T4 gp32 for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    gp32
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Platinum Gate TALEN Kit

    Plasmid Kit

    #1000000043

    Purpose

    Enables construction of highly-active Platinum TALENs using two-step Golden Gate cloning method. Platinum TALENs have variable TALE repeats with either +136/+63 or +153/+47 TALE scaffolds.

    Depositor

    Takashi Yamamoto

    Available Since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

12. RHO3

    Bacterial Strain

    #124700

    Purpose

    E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

    Depositor

    Herbert Schweizer

    Article

    Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

    Bacterial Resistance

    Dap (400 µg/ml)

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

13. pTRKH3-ermGFP

    Plasmid

    #27169

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
    

    Expression

    Bacterial

    Mutation

    presence of an additional 183-bp region at N term…

    Available Since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. elavl3:hArr-TEV;he1.1:YFP

    Plasmid

    #218855

    Purpose

    D. rerio elavl3 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hArr
    

    Tags

    human Arrestin-Tobbaco Etch Virus protease fusion…

    Expression

    Bacterial

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry

    Plasmid

    #218860

    Purpose

    10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hGCG-ICAM(1235)-nrxn1b
    

    Expression

    Bacterial

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS_NP_EBOV

    Plasmid

    #103049

    Purpose

    Encodes Ebola virus NP gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    NP gene of Ebola virus (NP Zaire ebolavirus, Mayinga)
    

    Expression

    Mammalian

    Mutation

    silent mutation (T to C) at position 928 of NP ge…

    Promoter

    CAG

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-PD-1 (Y223F, Y248F)-mGFP

    Plasmid

    #180790

    Purpose

    Expression of human PD-1 (Y223F, Y248F) fused to mEGFP

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    PD-1 (Y223F, Y248F) (PDCD1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Mutation

    PD-1 (Y223F, Y248F)

    Promoter

    SFFV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N1 Dynamin1 wt

    Plasmid

    #120313

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCXLE-EGFP

    Plasmid

    #27082

    Purpose

    Integration-free (episomal) expression of EGFP

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    eGFP
    

    Expression

    Mammalian

    Available Since

    March 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS_VP30_EBOV

    Plasmid

    #103051

    Purpose

    Encodes Ebola virus VP30 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    VP30 gene of Ebola virus (VP30 Zaire ebolavirus, Mayinga)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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