We narrowed to 10,362 results for: Coli
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Plasmid#173783PurposeThe 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.DepositorInsert186 Integrase
UseSynthetic BiologyExpressionBacterialMutationE59G - please see depositor commentsAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper_W
Plasmid#173784PurposeThe Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.DepositorInsertWphi Integrase
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper_D
Plasmid#173781PurposeThe D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.DepositorInsertD145 Integrase
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGR-ECK120010783
Plasmid#46009DepositorInsertECK120010783 Terminator
UseSynthetic BiologyExpressionBacterialPromoterPBADAvailable SinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
TDGS50
Plasmid#183189PurposeTEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoterDepositorInsertsDivIVA-GFP(tevR M0050)
TEV-S219V
ExpressionBacterialAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3-AD010
Plasmid#116851PurposeExpresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010DepositorInsertsAraC
alpha-HL
mScarletI
UseSynthetic BiologyExpressionBacterialPromoterpBADAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEL1324
Plasmid#139874PurposeExpression plasmid for WT LetB (without TM region) for cryo and negative stain EMDepositorInsertLetB
ExpressionBacterialMutationLetB(43-877)-6xHisAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGR-ECK125108723
Plasmid#46008DepositorInsertECK125108723 Terminator
UseSynthetic BiologyExpressionBacterialPromoterPBADAvailable SinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKS012
Plasmid#65465PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and mCherryDepositorInsertNtag-mCherry
UseSynthetic BiologyTagsFLAGExpressionBacterialPromoterpl-TetOAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL4
Plasmid#72988PurposeRBS_H5DepositorInsertH5 RBS
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL8
Plasmid#72992PurposeRBS_L1DepositorInsertL1 RBS
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-L1U1H09
Plasmid#73008PurposeL1U1H09 terminatorDepositorInsertL1U1H09 terminator
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZH512
Plasmid#102666PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDUET-ctCPR-BovAMO
Plasmid#20113DepositorInsertp450 reductase, and amorphadiene oxidase
UseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTH848-gst-disec
Plasmid#89589PurposePlasmid for bacterial expression of His-tagged GST encoded by non-preferred codonsDepositorArticleInsertglutathione-S transferase codon-dis-optimised for bacterial expression
Tags8xhistidineExpressionBacterialPromoterT7Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGR-ECK120010800
Plasmid#46010DepositorInsertECK120010800 Terminator
UseSynthetic BiologyExpressionBacterialPromoterPBADAvailable SinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKB234.2
Plasmid#170023PurposeEncodes LacI-controlled expression of surface-displayed hBD3 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
hBD3 Δ alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceDec. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDUET-ctCPR-sAMO
Plasmid#20111DepositorInsertp450 reductase and codon optimized amorphadiene oxidase
UseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pZH511
Plasmid#102665PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGC4780
Plasmid#113408PurposeTranslation reporter with inducible translation coupling (high native GFP expression)DepositorInsert158_33121123_1
UseSynthetic BiologyTagsfused to codon deptimized sfGFP with Flexible lin…ExpressionBacterialAvailable SinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pES E38A
Plasmid#13049DepositorInsertMutS
TagsHis6ExpressionBacterialMutationmutation Glu38 to AlaAvailable SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ClbN-NHis
Plasmid#51497PurposeNRPS module containing CATE domains with N-terminal 6His tagDepositorInsertClbN
Tags6x His tag and T7 tagExpressionBacterialMutationcontains aa30-1094 of NP_754347.1PromoterT7Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDUET-ctCPR-A13AMO
Plasmid#20114DepositorInsertp450 reductase and amorphadiene oxidase
UseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDUET-ctCPR-trAMO
Plasmid#20112DepositorInsertp450 reductase and truncated amorphadiene oxidase
UseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAK
Plasmid#19490DepositorInsertdnaK molecular chaperone DnaK
TagsGFPExpressionMammalianAvailable SinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEc-crRNA1
Plasmid#170088Purposeencodes E. coli type I-E single spacer arrayDepositorInsertE. coli type I-E single spacer array
UseCRISPR and Synthetic BiologyPromoterJ23119Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
circRAJ31-Toehold_mutant
Plasmid#69930PurposeExpresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31.DepositorInsertplTet-circRAJ31_toeholdmutant
UseSynthetic BiologyMutation4 bases mutated in toehold of riboregulatorPromoterPltetO1-2Available SinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTEI122
Plasmid#126062PurposeModule 0 vector of PlaMiNGo toolkitDepositorInsertccdB Cassette
UseSynthetic Biology; Module 0Available SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS DNAK
Plasmid#19544DepositorInsertdnaK molecular chaperone DnaK
TagsHisExpressionMammalianAvailable SinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL9
Plasmid#74098PurposeTL9 RBS (AAAAAG)DepositorInsertTL9 RBS
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only