We narrowed to 21,339 results for: pET

1. pet151D-TOPO-BROX 1-411; L212D

   Plasmid

   #89864

   Purpose

   Expresses full length human BROX with mutation L212D in bacteria; internal ID WISP11-300

   Depositor

   Wesley Sundquist

   Article

   Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

   Insert

   BROX (BROX Human)
   

   Use

   Tags

   His Tag, V5 tag

   Expression

   Bacterial

   Mutation

   mutated leucine 212 to aspartic acid

   Promoter

   T7

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET151-GFP-PP-RPN2 (918-953)

   Plasmid

   #73743

   Purpose

   Expression of GFP-PP-RPN2 (918-53) fusion in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN2 (RPN2 Human)
   

   Use

   Tags

   6x His and GFP

   Expression

   Bacterial

   Mutation

   Construct possesses only residues 918-953 of RPN2

   Promoter

   T7

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pET151-GFP-PP-RPN2 (932-953)

   Plasmid

   #73745

   Purpose

   Expression of GFP-PP-RPN2 (932-953) fusion in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN2 (RPN2 Human)
   

   Use

   Tags

   6x His and GFP

   Expression

   Bacterial

   Mutation

   Construct possesses only residues 932-953 of RPN2

   Promoter

   T7

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pET151-GFP-PP-RPN2 (918-947)

   Plasmid

   #73747

   Purpose

   Expression of GFP-PP-RPN2 (918-947) fusion in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN2 (RPN2 Human)
   

   Use

   Tags

   6x His and GFP

   Expression

   Bacterial

   Mutation

   Construct possesses only residues 918-947 of RPN2

   Promoter

   T7

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pET151-GFP-PP-RPN2 (797-953)

   Plasmid

   #74228

   Purpose

   [Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN2 (RPN2 Human)
   

   Use

   Tags

   6x His and GFP

   Expression

   Bacterial

   Mutation

   Construct possesses only residues 918-953 of RPN2

   Promoter

   T7

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pET151-Vps4(101-437) L151D

   Plasmid

   #87736

   Purpose

   Bacterial expression of a monomeric variant of the S. cerevisiae Vps4 AAA ATPase cassette

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Use

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100, mutated Leu 151 to Asp

   Promoter

   T7

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-Hook3 Q147A, M151A, I154A

   Plasmid

   #74616

   Purpose

   Expresses a triple mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Q147A, M151A, I154A

   Promoter

   T7

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-Hook3 N68A, W69A, K77A

   Plasmid

   #74619

   Purpose

   Expresses a triple mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET151-hUCH37 (ISF1)(M148D/F149D)

   Plasmid

   #71388

   Purpose

   Expression of hUCH37 (M148D/F149D) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Insert

   hUCH37 (ISF1) M148D/F149D (UCHL5 Human)
   

   Use

   Tags

   HIS-TEV sequence

   Expression

   Bacterial

   Mutation

   changed methionine 148 to aspartate, phenylalanin…

   Promoter

   T7

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET151-hUCH37 (ISF1)(M148A/F149A)

    Plasmid

    #61933

    Purpose

    Expression of hUCH37 (M148A/F149A) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) (UCHL5 Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Methionine 148 and Phenylalanine 149 were mutated…

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)

    Plasmid

    #61941

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-117) (NFRKB Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 117, stop codon introdu…

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

12. 213-petDuet1-GBD-LUMP--cdc42

    Plasmid

    #65973

    Purpose

    Dual expression of GBD-LUMP and cdc42

    Depositor

    Gerard Marriott

    Article

    Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

    Inserts

    LUMP
    cell division cycle 42 (CDC42 Human)
    

    Use

    Tags

    GBD (GTPase binding domain from ACK1; residues 44…

    Expression

    Bacterial

    Mutation

    constitutively active Cdc42 (Q61L)

    Promoter

    T7

    Available Since

    July 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET11+PCNA3-CYP119(D77R/T214V)

    Plasmid

    #66138

    Purpose

    Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    D77R, T214V

    Promoter

    T7 promoter

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-22HT-MSI1(7-192)Q30D

    Plasmid

    #60346

    Purpose

    Q30D mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    Q30D; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Dec. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pET-22HT-MSI1(7-192)R53E

    Plasmid

    #60352

    Purpose

    R53E mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    R53E; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pET-22HT-MSI1(7-192)G64A

    Plasmid

    #60351

    Purpose

    G64A mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    G64A; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pET-22HT-MSI1(7-192)H83L

    Plasmid

    #60350

    Purpose

    H83L mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    H83L; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pET-22HT-MSI1(7-192)G35D

    Plasmid

    #60347

    Purpose

    G35D mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    G35D; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pET-22HT-MSI1(7-192)G35E

    Plasmid

    #60348

    Purpose

    G35E mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    G35E; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pET-22HT-MSI1(7-192)H83F

    Plasmid

    #60349

    Purpose

    H83F mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    H83F; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pET28a-ybbR-HIS-Spec(x2)-DocI

    Plasmid

    #58713

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    Spec(x2)-DocI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET15b/GgVcl 1-851 A50I mutation

    Plasmid

    #46174

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

    Insert

    Vcl 1-851 A50I (VCL Chicken)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 1-851 A50I mutation (talin binding domain mut…

    Promoter

    T7

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET15b/GgVcl 1-258 A50I mutation

    Plasmid

    #46175

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

    Insert

    Vcl 1-258 A50I (VCL Chicken)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 1-258 (VD1) A50I mutation; talin binding defic…

    Promoter

    T7

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28b(+)_S. cerevisiae Hbs1(162-611)

    Plasmid

    #39337

    Depositor

    SeWon Suh

    Insert

    Elongation factor 1 alpha-like protein
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pET-21a(+)-DR RecR(K23A/R27A)

    Plasmid

    #12619

    Depositor

    SeWon Suh

    Article

    Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

    Insert

    Deinococcus radiodurans RecR (recR Deinococcus radiodurans)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Lysine 23 to Alanine, and Arginine 27 to …

    Promoter

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p141 pETF-GST RanBP10 G308R

    Plasmid

    #11094

    Depositor

    Ramesh Shivdasani

    Insert

    RanBP10 (Ranbp10 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G308R

    Promoter

    Available Since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFS_1113_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid

    #184741

    Purpose

    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23103

    Depositor

    Randall Platt

    Article

    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

    Insert

    FsRT-Cas1(opt)-Cas2(opt)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sequence codon optimized for expression in E. coli

    Promoter

    pTetA

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFS_1142_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader1-ARRAY1-DR1-FaqI_Term_NotI

    Plasmid

    #184742

    Purpose

    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 1 transcribed by BBa_J23103

    Depositor

    Randall Platt

    Article

    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

    Insert

    FsRT-Cas1(opt)-Cas2(opt)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sequence codon optimized for expression in E. coli

    Promoter

    pTetA

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid

    #184739

    Purpose

    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106

    Depositor

    Randall Platt

    Article

    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

    Insert

    FsRT-Cas1(opt)-Cas2(opt)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sequence codon optimized for expression in E. coli

    Promoter

    pTetA

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid

    #184740

    Purpose

    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109

    Depositor

    Randall Platt

    Article

    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

    Insert

    FsRT-Cas1(opt)-Cas2(opt)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sequence codon optimized for expression in E. coli

    Promoter

    pTetA

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET His6 TEV LIC cloning vector (2B-T)

    Plasmid

    #29666

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET StrepII TEV LIC cloning vector (1R)

    Plasmid

    #29664

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Strep II and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET GFP LIC cloning vector (u-msfGFP)

    Plasmid

    #29772

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    SiriusGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET mCherry LIC cloning vector (u-mCherry)

    Plasmid

    #29769

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET His6 TEV LIC cloning vector (1B)

    Plasmid

    #29653

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET His6 LIC cloning vector (2Bc-T)

    Plasmid

    #37236

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET Flag TEV LIC cloning vector (2L-T)

    Plasmid

    #29715

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    FLAG-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid

    #73440

    Purpose

    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE-P4A6-vioC-P3A2-vioD
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET tdTomato LIC cloning vector (H6-tdTomato)

    Plasmid

    #86928

    Purpose

    pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and td…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET mOrange LIC cloning vector (u-mOrange)

    Plasmid

    #29770

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    mOrange

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET GFP LIC cloning vector (2GFP-T)

    Plasmid

    #29716

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, SiriusGFP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET Flag TEV LIC cloning vector (1L)

    Plasmid

    #29662

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Flag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET His10 TEV LIC cloning vector (2B-T-10)

    Plasmid

    #78173

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    10xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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44. pET mCerulean LIC cloning vector (H6-mCerulean)

    Plasmid

    #29726

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and mC…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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45. pET tdTomato LIC cloning vector (u-tdTomato)

    Plasmid

    #86926

    Purpose

    pET LIC cloning vector for yORF-tdTomato

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    tdTomato

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. HIV-1 integrase F185K/C280S in pET-15b

    Plasmid

    #61669

    Purpose

    HIV-1 integrase F185K/C280S in pET-15b

    Depositor

    Robert Craigie

    Article

    Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

    Insert

    HIV-1 integrase F185K/C280S
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    T7 promotor

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

47. pET mCitrine LIC cloning vector (u-mCitrine)

    Plasmid

    #29771

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    mCitrine

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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48. His-TEV-Aurein1.2_(+36GFP)-LPETG-His

    Plasmid

    #71755

    Purpose

    His-TEV-Aurein1.2+36GFP-LPETG-His expression in E.Coli

    Depositor

    David Liu

    Article

    Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

    Insert

    Aurein1.2_(pos36_GFP)-LPETG-His
    

    Use

    Tags

    6XHis, Sortase (LPETG), and TEV Protease

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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49. pET mCerulean LIC cloning vector (u-mCerulean)

    Plasmid

    #29773

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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50. pET PPL His6 LIC cloning vector (2J-T)

    Plasmid

    #37182

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6 and PPL

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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