We narrowed to 17,230 results for: EMB

1. pEx-deletion-erm

   Plasmid

   #172213

   Purpose

   For allelic exchange in erythromycin-sensitive Prevotella copri strains

   Depositor

   Till Strowig

   Article

   Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

   Type

   Empty backbone

   Use

   Allelic exchange in prevotella copri

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGene-TAP-GBP1

   Plasmid

   #138087

   Purpose

   Plasmid with TAP-GBP1 ORF

   Depositor

   Eva Frickel

   Article

   Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

   Insert

   human GBP1 (GBP1 Human)
   

   Use

   Tags

   Tandem affinity purification (TAP) tag

   Expression

   Mammalian

   Mutation

   Promoter

   Gal4-E1b

   Available since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTM-BPL

   Plasmid

   #173766

   Purpose

   Fluorescent labeling of the nuclear envelope

   Depositor

   Shinji Sueda

   Article

   Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14

   Insert

   PDGF receptor TM domain, Biotin protein ligase
   

   Use

   Tags

   FLAG and Signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBCCP-GFP-NLS

   Plasmid

   #173923

   Purpose

   Fluorescent labeling of the nuclear envelope

   Depositor

   Shinji Sueda

   Article

   Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14

   Insert

   Biotin carboxyl carrier protein, AcGFP1
   

   Use

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV_Stargazin-mTurquoise2-iLID

   Plasmid

   #161001

   Purpose

   Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion

   Depositor

   Sean R Collins

   Article

   Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

   Insert

   Stargazin-mTurquoise2-iLID
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSP1E3D1_D73C

   Plasmid

   #39328

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

   Insert

   MSP1E3D1_D73C (APOA1 synthetic gene)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   cys mutant of MSP1E3D1

   Promoter

   T7

   Available since

   Sept. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pNDGG003

   Plasmid

   #231316

   Purpose

   BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Tc-par-ELT4; GG assembly from pOGG010, pOGG012, pOGG014, pOGG042, and pOGG004, TcR

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. MmXYRS-4xM15

   Plasmid

   #155343

   Purpose

   Plasmid encoding the translational pair that enables efficient incorporation of multiple phenylalanine-based amino acids in response to the amber codon into proteins in mammmalian cells.

   Depositor

   Irene Coin

   Article

   Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

   Insert

   Humanized MmXYRS/tRNAM15
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/U6

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Tollip

   Plasmid

   #178050

   Purpose

   GFP fused to human Tollip cDNA. Localisation and expression in mammalian cells.

   Depositor

   David Tumbarello

   Article

   Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

   Insert

   Toll-Interacting Protein (TOLLIP Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. 5HT6-YFP-Inp54p

    Plasmid

    #96812

    Purpose

    cilia-membrane-targeted inositol polyphosphate 5-phosphatase

    Depositor

    Takanari Inoue

    Article

    Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

    Insert

    5HT6-YFP-Inp54p
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Opto-ACTU−

    Plasmid

    #198869

    Purpose

    Optogenetic recruitment construct to increase inner membrane surface charge

    Depositor

    Peter Devreotes

    Article

    Banerjee et al Nat Cell Biol. 2022 Oct;24(10):1499-1515

    Insert

    Rad17 polyanionic tail (-14)
    

    Use

    Synthetic Biology

    Tags

    CRY2PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-cMSP1E3D1

    Plasmid

    #199772

    Purpose

    Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

    Insert

    cMSP1E3D1
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    Extended cMSP1D1; external His-Tags flanking the …

    Promoter

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNDGG050

    Plasmid

    #231319

    Purpose

    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Gm-par-ELT4; GG assembly from pOGG004, pOGG009, pOGG010, pOGG012, and pOGG014, GmR

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEx-deletion-tet

    Plasmid

    #172214

    Purpose

    For allelic exchange in tetracyclin-sensitive Prevotella copri strains

    Depositor

    Till Strowig

    Article

    Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

    Type

    Empty backbone

    Use

    Allelic exchange in prevotella copri

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCherry-mNrp1

    Plasmid

    #21934

    Depositor

    Guido Serini

    Article

    Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

    Insert

    neuropilin 1 (Nrp1 Mouse)
    

    Use

    Tags

    Cherry and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pLV_ADRB2-mTurquoise2-iLID

    Plasmid

    #161002

    Purpose

    Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion

    Depositor

    Sean R Collins

    Article

    Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

    Insert

    ADRB2-mTurquoise2-iLID
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-MLC2 WT

    Plasmid

    #195235

    Purpose

    Mammalian expression of GFP tagged MLC2. Wild-type

    Depositor

    Keith Mostov, David Bryant

    Article

    Yu et al EMBO Rep. 2008 Sep;9(9):923-9. doi: 10.1

    Insert

    MLC2 (MYL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. 5HT6-YFP-PIPK

    Plasmid

    #96810

    Purpose

    cilia-membrane-targeted phosphatidylinositol 4-phosphate 5-kinase

    Depositor

    Takanari Inoue

    Article

    Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

    Insert

    5HT6-YFP-PIPK
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-C2-GGA2

    Plasmid

    #199172

    Purpose

    Expresses GFP-GGA2 fusion in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

    Insert

    GGA2 (GGA2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Ala 23 Thr substitution; silent substitution in c…

    Promoter

    CMV

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmCherry-C1-pmDH

    Plasmid

    #67897

    Purpose

    Expresses a fusion of mCherry with the membrane located DH domain (1-347) of p63RhoGEF in mammalian cells

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    mCherry-pmDH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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