We narrowed to 42,279 results for: Tro;

1. pCW57.1 tTA SDHB

   Plasmid

   #177977

   Purpose

   All-in-one dox-repressible expression of cDNA

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   SDHB (SDHB Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Silent point mutations to SDHB sgRNA target

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. OPA1-isoform5

   Plasmid

   #70177

   Purpose

   Mammalian expression of hsOPA1 isoform 5

   Depositor

   David Chan

   Article

   Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

   Insert

   hsOPA1 isoform 5 (OPA1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. HSC1-HS4-D4Z4c-GiP

   Plasmid

   #58541

   Purpose

   Derived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoter

   Depositor

   James Ellis

   Article

   Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

   Inserts

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   D4Z4 repeat, partial
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   Human EF1-alpha

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-Rab35 WT

   Plasmid

   #47424

   Depositor

   Peter McPherson

   Article

   Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

   Insert

   Rab35 (RAB35 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. SID4x-dCas9-KRAB

   Plasmid

   #106399

   Purpose

   Expresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domain

   Depositor

   Jason Gertz

   Article

   Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

   Inserts

   SID4x (MXD1 Human)
   KRAB (ZNF10 Human)
   

   Tags

   FLAG, HA, and NLS

   Expression

   Mammalian

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSCL758

   Plasmid

   #219504

   Purpose

   Express Eco3RT from a CAG promoter; also express an msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

   Insert

   msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
   

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRubiC-T2A-Cas9

   Plasmid

   #75347

   Purpose

   Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   mCherry (via t2a)

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe-puro-mRipk3

   Plasmid

   #78830

   Purpose

   retroviral expression of mRIPK3

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK3 (Ripk3 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   5'LTR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry

   Plasmid

   #74309

   Purpose

   Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Insert

   TRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Human, Rat)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-SspB-HaloTag-TIAM1-DHPH

    Plasmid

    #176127

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW57.1 tTA UQCRC2

    Plasmid

    #177978

    Purpose

    All-in-one dox-repressible expression of cDNA

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    UQCRC2 (UQCRC2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Silent point mutations to UQCRC2 sgRNA target

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMM643

    Plasmid

    #112533

    Purpose

    pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    ChuA
    luxCDABE
    

    Expression

    Bacterial

    Mutation

    A17P

    Promoter

    J23107, PL(HrtO), and ProD

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV[Exp]-Neo-EF1A>Tet3G

    Plasmid

    #184379

    Purpose

    The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Tet3G
    

    Use

    Lentiviral

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. OPA1-isoform2

    Plasmid

    #70174

    Purpose

    Mammalian expression of hsOPA1 isoform 2

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 2 (OPA1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. xlox dNGFR-TERT

    Plasmid

    #69805

    Purpose

    MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a tNGFR marker for tracking

    Depositor

    David Ott

    Article

    Andersen et al AIDS Res Hum Retroviruses. 2007 Mar;23(3

    Insert

    hTERT reverse transcriptase (TERT Human)
    

    Use

    Retroviral

    Tags

    none

    Promoter

    MLV promoter

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. OPA1-isoform3

    Plasmid

    #70175

    Purpose

    Mammalian expression of hsOPA1 isoform 3

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 3 (OPA1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

17. OPA1-isoform4

    Plasmid

    #70176

    Purpose

    Mammalian expression of hsOPA1 isoform 4

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 4 (OPA1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

18. OPA1-isoform6

    Plasmid

    #70178

    Purpose

    Mammalian expression of hsOPA1 isoform 6

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 6 (OPA1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pLentiCRISPRv1_SDHB

    Plasmid

    #177981

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting SDHB

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting SDHB (SDHB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-neo-TET

    Plasmid

    #51286

    Purpose

    expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    U6 (RNU6-1 Human)
    

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Promoter

    U6 upstream enhancer sequence

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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