We narrowed to 152,634 results for: des

1. PcDNA-DEST53-CypB-Del2 N-term GFP

   Plasmid

   #36133

   Depositor

   Patrick Van Oostveldt

   Article

   Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

   Insert

   cyclophilin B (PPIB Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains CypB AA 38-210

   Promoter

   CMV

   Available since

   Nov. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

2. PcDNA-DEST53-CypB-Del1 N-term GFP

   Plasmid

   #36131

   Depositor

   Patrick Van Oostveldt

   Article

   Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

   Insert

   cyclophilin B (PPIB Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains amino acids 37-216 of cyclophilin B; F21…

   Promoter

   CMV

   Available since

   Nov. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

3. PcDNA-DEST53-CypB-Nucl2 N-term GFP

   Plasmid

   #36125

   Depositor

   Patrick Van Oostveldt

   Article

   Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

   Insert

   cyclophilin B (PPIB Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains CypB AA 34-211

   Promoter

   CMV

   Available since

   Nov. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

4. PcDNA-DEST53-CypB-Del3 N-term GFP

   Plasmid

   #36135

   Depositor

   Patrick Van Oostveldt

   Article

   Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

   Insert

   cyclophilin B (PPIB Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains amino acids 39-210 of cyclophilin B

   Promoter

   CMV

   Available since

   Nov. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

5. MTK Lentivirus Multi Cassette Destination Vector (pAN2424)

   Plasmid

   #194221

   Purpose

   Backbone for constructing multi casette expression plasmids using the Mammalian Tookit

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MTK Lentivirus Single Cassette Destination Vector (pAN2414)

   Plasmid

   #194220

   Purpose

   Backbone for constructing single casette expression plasmids using the Mammalian Tookit

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHR-ON VIPER CAR (anti-CD19)-final design

   Plasmid

   #192117

   Purpose

   Lentiviral deliviery of ON VIPER CAR (anti-CD19)-final design

   Depositor

   Wilson Wong

   Article

   Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

   Insert

   pHR-pEF1a-VIPER CAR (anti-CD19scFV_CD28_NS3_41BB_CD3z)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3_attR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA_DEST

   Plasmid

   #194386

   Purpose

   Gateway Destination vector for GPCR split TEV assays with V2R tail

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   attR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Desmoplakin I affinity mutant (F40-based tension sensor)

   Plasmid

   #119190

   Purpose

   The affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   TCE

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

    Plasmid

    #105643

    Purpose

    Co-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermis

    Depositor

    Sandra Rieger

    Article

    Lisse et al Proc Natl Acad Sci U S A. 2016 Apr 12;11

    Insert

    Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pEF5/FRT-DEST EVC2-TEV-YFP-Flag

    Plasmid

    #41004

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Use

    Tags

    TEV-YFP-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDest-Tol2 (Chien)-Krt4:Gal4VP16:tdTomato:5xUAS_MCS

    Plasmid

    #105649

    Purpose

    tdTomato expression in differentiated keratinocytes of the periderm layer and during early larval stages also in the basal layer of the epidermis

    Depositor

    Sandra Rieger

    Insert

    Krt4:Gal4VP16:tdTomato:5xUAS_MCS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

13. hu-TLR4 299/399 dual snp flag pDEST40

    Plasmid

    #42648

    Purpose

    destination vector for expression of human TLR4 299/399 SNP in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 299/399 SNP (TLR4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D299G and T399I

    Promoter

    CMV

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268

    Plasmid

    #69224

    Purpose

    Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS and Zif268

    Expression

    Mammalian

    Mutation

    Arginine 1335 is changed to Lysine

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEF5/FRT-DEST EVC2dW-TEV-YFP-Flag

    Plasmid

    #41006

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Use

    Tags

    TEV-YFP-Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1177-1220

    Promoter

    Available since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

16. Desmoplakin II donor only control (FL-based tension sensor)

    Plasmid

    #118723

    Purpose

    The donor only (YPet(short)) control for the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin II-[YPet(short)-FL-mCherry(Y72L)] (internal-1353) (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-FL-mCherry(Y72L)

    Expression

    Mammalian

    Mutation

    inserted FL-based tension sensor module after aa1…

    Promoter

    CMV

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry

    Plasmid

    #69232

    Purpose

    Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

    Plasmid

    #229819

    Purpose

    A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-Tet-Off-FLEX-DEST (JDW 1186)

    Plasmid

    #229820

    Purpose

    An AAV, tet-off, gateway compatible destination vector with cre dependent expression of the insert. EFS driving destablized rTA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)

    Plasmid

    #229831

    Purpose

    A gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge