308,349 results

1. AAV-EFS-GFP

   Plasmid

   #170176

   Purpose

   Expresses GFP under the control of EF1a short promoter

   Depositor

   Xiaojun Lian

   Article

   Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available since

   July 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBiT3.1-N_SCAP:1-905

   Plasmid

   #211228

   Purpose

   Mammalian expression of full-length human SCAP isoform 2. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   SCAP:M1-D905 (SCAP Human)
   

   Use

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   isoform 2. V798I natural variant (VAR_012203)

   Promoter

   CMV

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. YIplac211-KAR2-msGFP2-HDEL

   Plasmid

   #160468

   Purpose

   Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signal

   Depositor

   Benjamin Glick

   Article

   Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

   Insert

   KAR2-msGFP2-HDEL
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEmerald-MCAK-C-7

   Plasmid

   #54161

   Purpose

   Localization: Microtubules/Kinesin, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   MCAK (KIF2C Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pET22-HT/plsA

   Plasmid

   #73625

   Purpose

   Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

   Depositor

   James Williamson, T. J. Kappock

   Article

   Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

   Insert

   adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223-ACVR2A

   Plasmid

   #23654

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ACVR2A (ACVR2A Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

7. RV-Cag-Dio-mCherry

   Plasmid

   #87664

   Purpose

   retroviral vector encoding cre dependent expression of membrane mCherry

   Depositor

   Oscar Marin

   Article

   Ciceri et al Nat Neurosci. 2013 Sep;16(9):1199-210. d

   Insert

   Mem-mCherry
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. AGPAT3_pCSdest

   Plasmid

   #53928

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   AGPAT3 (AGPAT3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

9. qTAG-C-moxGFP-Blast-H2BC11

   Plasmid

   #207757

   Purpose

   Donor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pcDNA5/FRT/TO HIS DNAJB2a

    Plasmid

    #19550

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB2a
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221_SLC25A48

    Plasmid

    #131995

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A48 (SLC25A48 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pMK1200

    Plasmid

    #84219

    Purpose

    Lentiviral expression of shRNAs, compatible with double-shRNA expression, EF1alpha promoter, mCherry

    Depositor

    Jonathan Weissman, Martin Kampmann

    Article

    Kampmann et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET11b-HA2

    Plasmid

    #165992

    Purpose

    Influenza HA ministem with removable HisTag

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    HA2
    

    Use

    Tags

    Foldon and HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-HA-DLC1-i4

    Plasmid

    #99534

    Purpose

    DLC1-isoform 4 expression plasmid

    Depositor

    Qian Tao

    Article

    Low et al Oncogene. 2011 Apr 21;30(16):1923-35. do

    Insert

    DLC1-isoform 4 (DLC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pICSL50010

    Plasmid

    #50310

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    C terminal Myc tag (4x Myc)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLIX403_Capture1_APOBEC_HA_P2A_mRuby

    Plasmid

    #183901

    Purpose

    Inducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

    Depositor

    Eugene Yeo

    Article

    Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    Capture1_APOBEC_HA_P2A_mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV5 DPC4 (1-514)

    Plasmid

    #14040

    Depositor

    Joan Massague

    Article

    Lagna et al Nature. 1996 Oct 31. 383(6603):832-6.

    Insert

    DPC4 (1-514) (SMAD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R515 was mutated into a stop codon. AGA to TGA. D…

    Promoter

    Available since

    May 10, 2007

    Availability

    Academic Institutions and Nonprofits only

18. mEmerald-Cx43-7

    Plasmid

    #54055

    Purpose

    Localization: Gap Junctions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Fong et al Autophagy. 2012 May 1;8(5):794-811. doi:

    Insert

    Cx43
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pJK-caCas9-NatMX-Neut5L-Ade2 drive

    Plasmid

    #89577

    Purpose

    caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Insert

    Ade2 gene drive
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-ms-Sox3

    Plasmid

    #50779

    Purpose

    retroviral expression of mouse Sox3

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

    Insert

    Sox3 (Sox3 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge