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pJZ001

Plasmid

#120230

Purpose

Contains intB13 integrase casette for knock-in to pCAP-BAC backbone

Depositor

Bradley Moore

Article

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Insert

intB13 integrase casette

Expression

Bacterial

Mutation

bla-g717a (amp resistance gene)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pPIC-mouseKir3.2-mC-S

Plasmid

#140745

Purpose

Expression of mouse Kir3.2 fused to mCherry and Streptag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2020 May 6. doi: 10.1021/a

Insert

Kir3.2 (Kcnj6 Mouse)

Tags

mCherry with Streptag II

Expression

Yeast

Promoter

AOX1

Available Since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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C239-E03: attB2r-IRES-ffLuc2-pA-attB3

Plasmid

#162919

Purpose

Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2 reporter using an internal ribosome entry sequence (IRES)

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

firefly luciferase with upstream IRES sequence

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3H08

Plasmid

#70996

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Nap1 (Nap1 Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
RNAPII_CTD27-37

Plasmid

#98686

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

heptads 27-37, residues 1773-1852

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ291

Plasmid

#129671

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

BthCas12b

Use

CRISPR; Gateway compatible bthcas12b entry clone

Expression

Plant

Mutation

BthCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pME-fli1b

Plasmid

#49019

Purpose

full length coding sequence for zebrafish fli1b gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

friend leukemia integration 1b (fli1rs Zebrafish)

Use

Gateway middle entry

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pEN_TT miRc2 3xFLAG

Plasmid

#83274

Purpose

Empty entry vector for cloning inducible mammalian expression and an in-frame N-terminal 3xFLAG

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Type

Empty backbone

Use

Entry vector for gateway cloning

Tags

3xFLAG

Promoter

TRE tight

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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IG101: pMVP (L5-L4) TRE promoter

Plasmid

#121729

Purpose

pMVP L5-L4 entry plasmid, contains TRE promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TRE promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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S8_attL1-N-term-EGFP-attL2

Plasmid

#128472

Purpose

EGFP entry clones

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

EGFP entry clones

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pME-BTK-PH

Plasmid

#67673

Purpose

Gateway entry clone containing BTK-PH with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

BTK-PH-NS

Use

Gateway multisite middle entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pTTR210K

Plasmid

#41750

Depositor

Jeff Gelles

Article

Thoresen et al Biochemistry. 2008 Sep 9;47(36):9514-21.

Insert

kinesin heavy chain (Khc Fly, E. coli)

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; R210K

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
A102-GAP-BoCCS-GRM

Plasmid

#123286

Purpose

Expression of Glo3 ΔAmpH (1-459) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Promoter

T5

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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6his R.3-AR GUSpET28a+/pLysS

Plasmid

#61157

Purpose

N-terminal His tagged round 3 aldehyde-resistant GUS expression vector

Depositor

Andrew Ellington, Ichiro Matsumura

Article

Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

Insert

GUSAR

Tags

6x His

Expression

Bacterial

Mutation

N66D, D151N, A219V, I396T, T480A, Q498R, D508E an…

Promoter

T7

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ290-D570A

Plasmid

#129673

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b-d570a entry …

Expression

Plant

Mutation

D570A; AacCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR221-WNT3

Plasmid

#79500

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WNT3 (WNT3 Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6 L1-R5

Plasmid

#62111

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(ATP1)/pENTR1A

Plasmid

#51993

Purpose

Gateway entry vector for Nano-lantern-based luminescent ATP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Use

Entry vector

Mutation

S257G in Rluc8

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-R4R3-mEGFP-N

Plasmid

#113746

Purpose

Gateway multi-site entry clone for second position mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP

Use

CRISPR; Gateway entry vector

Tags

mEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b G12D/C185S

Plasmid

#83165

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D/C185S mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Mutation

G12D/C185S

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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