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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 12,861 results for: sequence

Showing: 2321 - 2340 of 12861 results

  1. pOpen-EcoRV

    Plasmid
    #165548
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GATATC and cleaves after T-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    EcoRV
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165548-plasmid-map-sequence-id-339428

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  2. pOpen-EcoRIR

    Plasmid
    #165504
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    EcoRI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165504-plasmid-map-sequence-id-338511

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  3. pOpen-XbaIR

    Plasmid
    #165567
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence TCTAGA and cleaves after T-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    XbaI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165567-plasmid-map-sequence-id-340099

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  4. pMSCV2.2-IRES- GFP-Opa1

    Plasmid
    #191971
    Purpose
    Plasmid for cloning reaction, containing OPA1 coding sequence.
    Depositor
    Luca Scorrano
    Insert
    GFP-Opa1
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    191971-plasmid-map-sequence-id-379642

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  5. pAAV-CAG-Flex-Rev-3xGFP

    Plasmid
    #122500
    Purpose
    Cre dependent GFP expression.
    Depositor
    Kimberly Ritola
    Insert
    3x GFP
    Use
    AAV
    Tags
    Expression
    Mutation
    each GFP has unique nt sequence
    Promoter
    Available since
    Jan. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    122500-plasmid-map-sequence-id-237802

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  6. pOpen-NcoIR

    Plasmid
    #165568
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence CCATGG and cleaves after C-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    NcoI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165568-plasmid-map-sequence-id-340100

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  7. pOpen-PstIM

    Plasmid
    #165536
    Purpose
    Methylase that recognizes the dsDNA sequence CTGCAG, methylates on A-5 on both strands, and protects DNA from cleavage by PstI.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    M.PstI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165536-plasmid-map-sequence-id-339126

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  8. hU6-Sa_tracr

    Plasmid
    #135973
    Purpose
    Contains tracrRNA-hU6 sequence for double sgRNA cloning
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    135973-plasmid-map-sequence-id-313948

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  9. pCAGGS-MTS-EGFP-V5

    Plasmid
    #200816
    Purpose
    expresses MTS-EGFP-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    EGFP
    Use
    Tags
    V5 and mitochondrial targeting sequence (MTS)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200816-plasmid-map-sequence-id-409411

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  10. pETM-11-DmPABPC1_501-634_J

    Plasmid
    #146641
    Purpose
    Bacterial Expression of HsPABPC1_501-634
    Depositor
    Elisa Izaurralde
    Insert
    HsPABPC1_501-634
    Use
    Tags
    Expression
    Bacterial
    Mutation
    2 silent mutations compared to the sequence given…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146641-plasmid-map-sequence-id-289087

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  11. pAc5.1B-lambdaN-HA-HsTNRC6C_G

    Plasmid
    #146374
    Purpose
    Insect Expression of HsTRC6C
    Depositor
    Elisa Izaurralde
    Insert
    HsTRC6C
    Use
    Tags
    Expression
    Insect
    Mutation
    two silent mutations C2349T and A2547C compared t…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146374-plasmid-map-sequence-id-288819

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  12. pDEST-Opa1

    Plasmid
    #191961
    Purpose
    Gateway Destination Vector, containing OPA1 coding sequence, for Gateway Cloning Technology
    Depositor
    Luca Scorrano
    Insert
    Opa1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191961-plasmid-map-sequence-id-379662

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  13. pOME0276M

    Plasmid
    #182614
    Purpose
    Reversed sequence of Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome. TO_7 Control for pOME0275
    Depositor
    Dirk Dittmer
    Insert
    Reverse compliment HHV8 TO_7 control
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182614-plasmid-map-sequence-id-359450

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  14. pOME0237M

    Plasmid
    #182599
    Purpose
    Reverse complement sequence of anti-PAN pOME0236 Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
    Depositor
    Dirk Dittmer
    Insert
    Reverse compliment of anti-PAN (pOME0236)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182599-plasmid-map-sequence-id-359434

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  15. EGF-bio-His

    Plasmid
    #53340
    Purpose
    Expresses full-length Pro-epidermal growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    EGF (EGF Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53340-plasmid-map-sequence-id-83070

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  16. pLentiGuide

    Plasmid
    #117986
    Purpose
    pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.
    Depositor
    Paul Khavari
    Article
    Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31
    Insert
    sgRNA backbone (F+E)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    117986-plasmid-map-sequence-id-235371

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  17. CTGF-bio-His

    Plasmid
    #52028
    Purpose
    Expresses full-length Connective tissue growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CTGF (CCN2 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52028-plasmid-map-sequence-id-80102

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  18. EFNB1-bio-His

    Plasmid
    #51749
    Purpose
    Expresses full-length Ephrin-B1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    EFNB1 (EFNB1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51749-plasmid-map-sequence-id-79334

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  19. ESAM-bio-His

    Plasmid
    #51751
    Purpose
    Expresses full-length Endothelial cell-selective adhesion molecule precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ESAM (ESAM Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51751-plasmid-map-sequence-id-79336

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Showing: 2321 - 2340 of 12861 results