We narrowed to 21,284 results for: cat.1

1. PB-GG-OCT4-1-5-PGK-Puro

   Plasmid

   #102893

   Purpose

   PiggyBac transposon system construct with 5 concatenated U6 promoter driven transcriptional cassettes for the activation of OCT4. Contains PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   OCT4-gRNAs-1-5-PGK-puro (POU5F1 )
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

   Plasmid

   #198072

   Purpose

   Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Tags

   GST-TEV-EGFP

   Expression

   Insect

   Mutation

   TBK1ΔCTD (res 1-666)

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-hSyn-GFP-IRES-mSNCA(1-95)

   Plasmid

   #185716

   Purpose

   AAV expression of GFP and C-terminal truncated (1-95 amino acid) mouse α-Synuclein from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (Snca Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   deleted AA 95-144

   Promoter

   hSyn

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p36-p37-p38-p40-pET-Duet-1

   Plasmid

   #175049

   Purpose

   Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunits 2-5 (RFC4, RFC2, RFC3, RFC5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV6-XL4 ASXL1 (1-479) 3x FLAG

   Plasmid

   #74262

   Purpose

   Cancer-associated ASXL1 truncation mutant in mammalian expression vector

   Depositor

   Anjana Rao

   Article

   Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

   Insert

   ASXL1 (ASXL1 Human)
   

   Use

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Contains ASXL1 amino acids 1-479

   Promoter

   5’LTR

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. PB-GG-OCT4-1-5-PGK-Puro

   Plasmid

   #102893

   Purpose

   PiggyBac transposon system construct with 5 concatenated U6 promoter driven transcriptional cassettes for the activation of OCT4. Contains PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   OCT4-gRNAs-1-5-PGK-puro (POU5F1 )
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

   Plasmid

   #198072

   Purpose

   Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Tags

   GST-TEV-EGFP

   Expression

   Insect

   Mutation

   TBK1ΔCTD (res 1-666)

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-hSyn-GFP-IRES-mSNCA(1-95)

   Plasmid

   #185716

   Purpose

   AAV expression of GFP and C-terminal truncated (1-95 amino acid) mouse α-Synuclein from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (Snca Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   deleted AA 95-144

   Promoter

   hSyn

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pN1-RnKIF5C(1-560,Δ6)-Halo-Flag

   Plasmid

   #188072

   Purpose

   Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags. The delta6 mutation removes the first 6 amino acids.

   Depositor

   Kristen Verhey

   Article

   Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

   Insert

   rat KIF5C (Kif5c Rat)
   

   Use

   Tags

   Flag and Halo

   Expression

   Mammalian

   Mutation

   aa1-560, deletion of aa2-6

   Promoter

   CMV

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST

    Plasmid

    #223765

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BNIP3 soluble part (BNIP3 Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (WT)

    Plasmid

    #223736

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    NIX (BNIP3L) soluble part (BNIP3L Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETDuet1_FUNDC1(1-50aa)-EGFP-TEV-GST (WT)

    Plasmid

    #223737

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    NIX (BNIP3L) soluble part (BNIP3L Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pN1-RnKIF5C(1-560,Δ6)-Halo-Flag

    Plasmid

    #188072

    Purpose

    Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags. The delta6 mutation removes the first 6 amino acids.

    Depositor

    Kristen Verhey

    Article

    Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

    Insert

    rat KIF5C (Kif5c Rat)
    

    Use

    Tags

    Flag and Halo

    Expression

    Mammalian

    Mutation

    aa1-560, deletion of aa2-6

    Promoter

    CMV

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST

    Plasmid

    #223765

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BNIP3 soluble part (BNIP3 Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (WT)

    Plasmid

    #223736

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    NIX (BNIP3L) soluble part (BNIP3L Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETDuet1_FUNDC1(1-50aa)-EGFP-TEV-GST (WT)

    Plasmid

    #223737

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    NIX (BNIP3L) soluble part (BNIP3L Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen

    Plasmid

    #196974

    Purpose

    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tag

    Depositor

    Lukas Kapitein

    Article

    Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Use

    Tags

    GST and mNeonGreen

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Cer(1-158)-beta-5 in pcDNAI/Amp

    Plasmid

    #55708

    Purpose

    An amino-terminal fragment of mCerulean was fused to Gbeta5. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    mCer(1-158)-beta5 (GNB5 Aequorea victoria, Human)
    

    Use

    Tags

    Cer(1-158) was fused to the amino terminus of Gbe…

    Expression

    Mammalian

    Mutation

    GBeta-5 was amplified via PCR, which added an N-t…

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid

    #129041

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA1 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YFP(1-158)-gamma-2 in pcDNAI/Amp

    Plasmid

    #55762

    Purpose

    An amino-terminal YFP fragment was fused to Ggamma2. When co-expressed with a carboxyl terminal YFP or CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    YFP(1-158)-gamma-2 (GNG2 Aequorea victoria, Human)
    

    Use

    Tags

    YFP(1-158) was fused to the amino terminus of Gga…

    Expression

    Mammalian

    Mutation

    YFP (1-158) includes a substitution of Met for Gl…

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge