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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 5,046 results for: Apoe

Showing: 2361 - 2380 of 5046 results

  1. LLPS2-CAHS2_PARRC::SspB (pBS1043)

    Plasmid
    #185290
    Purpose
    For the mammalian expression of the tardigrade protein CAHS2_PARRC attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    CAHS2_PARRC
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185290-plasmid-map-sequence-id-363897

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  3. p1A Prk-

    Plasmid
    #162694
    Purpose
    p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    H. neapolitanus CbbLS and S. elongatus Prk
    Use
    Tags
    N terminal 6x His tag on PRK
    Expression
    Bacterial
    Mutation
    Prk inactive (K20M S21A native protein, this map …
    Promoter
    PLtet0-1 promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162694-plasmid-map-sequence-id-324149

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  4. LentiGuide-Puro-NCOA7g3 (BB24)

    Plasmid
    #139459
    Purpose
    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker
    Depositor
    Caroline Goujon
    Article
    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.
    Insert
    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a / U6
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139459-plasmid-map-sequence-id-308470

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  11. pHRIG-Akt1

    Plasmid
    #53583
    Purpose
    lentiviral expression of constitutively active human Akt1 and EGFP
    Depositor
    Heng Zhao
    Article
    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12
    Insert
    cAkt1 (AKT1 Human)
    Use
    Lentiviral
    Tags
    HA and IRES-EGFP
    Expression
    Mammalian
    Mutation
    constitutively active (myristoylated version)
    Promoter
    CMV
    Available since
    Oct. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    53583-plasmid-map-sequence-id-448192

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  17. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

    Plasmid
    #159107
    Purpose
    The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).
    Depositor
    Thomas Tuschl
    Article
    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.
    Insert
    NSP12
    Use
    Tags
    His-SUMO tag
    Expression
    Bacterial
    Mutation
    None
    Promoter
    T7
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    159107-plasmid-map-sequence-id-310025

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  19. pGEM-2t

    Plasmid
    #159752
    Purpose
    CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    sgRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159752-plasmid-map-sequence-id-313824

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  20. pYEE

    Plasmid
    #159750
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159750-plasmid-map-sequence-id-313819

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Showing: 2361 - 2380 of 5046 results