We narrowed to 4,065 results for: SPL;

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SpyCatcher-DIIG

Plasmid

#201196

Purpose

Expresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteria

Depositor

Vesa Hytönen

Article

Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

Insert

SpyCatcher-DIIG

Tags

HisTag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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SpyCatcher-SWG

Plasmid

#201194

Purpose

Expresses a peptide from the active site of furin genetically fused to SpyCatcher001 in bacteria

Depositor

Vesa Hytönen

Article

Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

Insert

SpyCatcher-SWG

Tags

HisTag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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SpyCatcher-PEED

Plasmid

#201195

Purpose

Expresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteria

Depositor

Vesa Hytönen

Article

Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

Insert

SpyCatcher-PEED

Tags

HisTag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_8xTEAD_luc2

Plasmid

#173005

Purpose

firefly luciferase reporter vector with 8x clustered TEAD binding sites

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

8xTEAD-luc2

Use

AAV and Luciferase

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSC-cUnaG-LPETG

Plasmid

#207635

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG

Tags

6xHis

Expression

Bacterial

Mutation

Amber stop codon at SC's critical lysine

Promoter

T7

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-ZpGal4DBD

Plasmid

#199199

Purpose

Insert to create ZpGal4DBD knock-ins, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-ZpGal4DBD

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-p65Zp

Plasmid

#199200

Purpose

Insert to create p65Zp knock-ins, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-p65Zp

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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GFP SMG1 T3550A/S3556A

Plasmid

#199572

Purpose

Expresses mutated SMG1 fused to GFP

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Tags

GFP

Expression

Mammalian

Mutation

T3550A and S3556A

Promoter

CMV

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-CeeIF4E3_1-215opt_AB

Plasmid

#148367

Purpose

Bacterial Expression of CeeIF4E3_1-215

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeeIF4E3_1-215

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-CeeIF4E3_30-215opt_AB

Plasmid

#148369

Purpose

Bacterial Expression of CeeIF4E3_30-215opt

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeeIF4E3_30-215opt

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Rtn2b-R60f-mCherry

Plasmid

#186607

Purpose

Third generation lentiviral vector expressing Reticulon 2 disease mutant R60f fused to mCherry

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 mutant R60f (RTN2 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

c.178insC (R60f)

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-NTD136-271-mNeon

Plasmid

#186604

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 136 to 271 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 136 to 271 (RTN2 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET PI-SceI C-His K301A

Plasmid

#195611

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutation

Depositor

Frederick Gimble

Article

He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

Insert

PI-SceI

Expression

Bacterial

Mutation

A822G, A823C, A824T

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET PI-SceI C-His

Plasmid

#195610

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter with a carboxyl-terminal histidine tag

Depositor

Frederick Gimble

Article

He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

Insert

PI-SceI

Expression

Bacterial

Mutation

T825C, C900G, A901C, A903C, T1131G, T1137C, T1227…

Promoter

T7

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pYI006

Plasmid

#170840

Purpose

FRO6p::GUSPlus::tOCS in pFP100

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

FRO6p::GUSPlus::tOCS

Expression

Plant

Promoter

FRO6

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-MBP-bhaB5

Plasmid

#167812

Purpose

Expresses His6- and MBP-tagged BhaB5 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

BhaB5

Tags

His6, MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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ppih.001.177.W133A

Plasmid

#137655

Purpose

Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Rajiv et al Biochem J. 2017 Oct 25;474(21):3689-3704

Insert

ppih (PPIH Human)

Tags

GST-(His)6-thrombin

Expression

Bacterial

Mutation

001-177-W133A

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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SPELL-pBabe-Vav2(DPZ) (Component 2-T2AP2A-Component 1)

Plasmid

#170488

Purpose

SPELL Vav2(DH-PH-ZnF). Two components cloned into the same plasmid with T2A/P2A sequence. Component 2-T2A-P2A-Component 1

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

FRB, Vav2(C-lobe), iFKBP, Vav2(N-lobe), mCherry

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-0-mTagBFP2(11x7)

Plasmid

#172064

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 0 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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DDX41_p.L237F_p.P238T_EGFP

Plasmid

#175845

Purpose

Expresses DDX41 with mutation

Depositor

Evan Braunstein

Article

Li et al Haematologica. 2016 Jun;101(6):e228-31.

Insert

DDX41 (DDX41 Human)

Tags

EGFP

Expression

Mammalian

Mutation

c.711G>T, p.L237F and c.712C>A, p.P238T

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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