We narrowed to 3,906 results for: SPL;

1. pGL2-p21 promoter-Luc

   Plasmid

   #33021

   Depositor

   Martin Walsh

   Article

   Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

   Insert

   p21 promoter (CDKN1A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   p21

   Available since

   April 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNA-CasRx_SD1-pSV40-TagBFP

   Plasmid

   #221004

   Purpose

   Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

   Depositor

   Mikko Taipale, Benjamin Blencowe

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   CasRx 30nt processed direct repeat with SD1 mutation
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   DR1 A>T

   Promoter

   U6

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4_CRE-CMVmin-luc2

   Plasmid

   #194384

   Purpose

   Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   6xCRE-CMVmin-luc2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV minimal

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHRm-NLS-dCas9-GFP11x7-NLS

   Plasmid

   #70224

   Purpose

   Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   NLS-dCas9-GFP11x7-NLS
   

   Use

   Tags

   GFP11x7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. BSD-CryC

   Plasmid

   #209672

   Purpose

   Expresses CRY2 fused with Split-TurboID-C and mCherry in mammalian cells

   Depositor

   Sanjeevi Sivasankar

   Article

   Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Lentiviral

   Tags

   Split-TurboID-C and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-NT-3/D15A

   Plasmid

   #73035

   Purpose

   Express an NT-3-BDNF hybrid neurotrophic factor in mammalian cells

   Depositor

   Pantelis Tsoulfas

   Article

   Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

   Insert

   NT-3/D15A (NTF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Mutated Aspartic acid in position 15 of the matur…

   Promoter

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcI-ts,ind+-(mtSSB delta20)

   Plasmid

   #186280

   Purpose

   Expresses Human Mitochondrial SSB in E. coli

   Depositor

   Kenneth Johnson

   Article

   Qian et al J Biol Chem. 2017 Aug 4;292(31):13068-13

   Insert

   Mitochondrial Single Stranded DNA Binding Protein (SSBP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   First 20 amino acids corresponding to probable mi…

   Promoter

   lambda

   Available since

   July 6, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. TRE-UBC-HLA-E SCT-T2A-GFP

   Plasmid

   #205461

   Purpose

   lentiviral plasmid for expression of HLA-E SCT

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   HLA-E SCT (HLA-E Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4-Chk1-Flag

   Plasmid

   #22894

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

   Insert

   Chk1 (CHEK1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAT15516_BEAR-GFP-2in1

    Plasmid

    #174082

    Purpose

    BEAR-GFP plasmid with a gRNA that targets its own plasmid

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCRISPR-E

    Plasmid

    #78852

    Purpose

    Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

    Depositor

    Phillip Abbosh

    Article

    Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Cas9-P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX317-EGFP

    Plasmid

    #115439

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRE-UBC-HLA-G SCT-T2A-GFP

    Plasmid

    #205462

    Purpose

    lentiviral plasmid for expression of HLA-G SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    HLA-G SCT (HLA-G Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid

    #133451

    Purpose

    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    TfR-sfGFP-myc tag-SpyCatcher003
    

    Use

    Tags

    GSSGS and myc tag

    Expression

    Mammalian

    Mutation

    Contains C20 and A23 mutations that improve plasm…

    Promoter

    CMV

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV7.1_3xFLAG-TAOK1

    Plasmid

    #172991

    Purpose

    constitutive expression of FLAG-tagged TAOK1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-TAOK1 (TAOK1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Intron-Tagging-pX330-Cas9-Blast

    Plasmid

    #159741

    Purpose

    Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

    Insert

    donor plasmid targeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV7.1_3xFLAG-TAOK2

    Plasmid

    #172992

    Purpose

    constitutive expression of FLAG-tagged TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-TAOK2 (TAOK2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-hPMCA2w/b

    Plasmid

    #47586

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2w/b (ATP2B2 Human)
    

    Use

    Tags

    EGFP-HA-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZT-AAVS1-R1

    Plasmid

    #52638

    Purpose

    TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

    Depositor

    Mahendra Rao, Jizhong Zou

    Article

    Luo et al Stem Cells Transl Med. 2014 May 15. pii:

    Insert

    Right AAVS1 TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZT-AAVS1-L1

    Plasmid

    #52637

    Purpose

    TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

    Depositor

    Mahendra Rao, Jizhong Zou

    Article

    Luo et al Stem Cells Transl Med. 2014 May 15. pii:

    Insert

    Left AAVS1 TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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