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Plasmid#92396PurposeCAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9_KRAB_2A_EGFP
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRCC-N
Plasmid#81192PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Human LFA-1 alpha
Plasmid#8630DepositorPublicationInsertLFA1 alpha (ITGAL Human)
ExpressionBacterialAvailable sinceJune 23, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCEP4-myc-HLA-A2
Plasmid#135504PurposeMammalian expression of myc-tagged HLA-A*02:01DepositorInsertHLA-A*02:01 (HLA-A Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
p133 pSV-STOP-luc
Plasmid#8390DepositorInsertfloxed STOP
UseCre/Lox and LuciferaseExpressionMammalianAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINO_NbH11-H4
Plasmid#155364PurposeExpresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2DepositorInsertH11-H4
TagsHis6ExpressionBacterialPromoterT7Available sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pYLsgRNA-OsU6b
Plasmid#66196Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorPromoterOsU6bAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ352
Plasmid#30539DepositorInsertttTi4348 region
ExpressionWormAvailable sinceJune 29, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWZL Neo Myr Flag PLK1
Plasmid#20589DepositorPublicationAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPR-aBEST
Plasmid#131464PurposeExpression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsStreptomyces codon optimized spCas9n (D10A)
streptomyces codon optimized tadA
UseCRISPR and Synthetic BiologyPromotertipAAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCT5-bac1.8
Plasmid#119871PurposeConstitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR'
sfGFP
UseSynthetic BiologyExpressionBacterialMutationSNP in 592 bpPromoterPserA promoter and Pveg promoter with CuO sequenceAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-mNeonGreen
Plasmid#99135PurposeAn AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the hSyn1 promoterDepositorInsertmNeonGreen
UseAAVExpressionMammalianPromoterhSyn1Available sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Multiplex Yeast Toolkit (MYT)
Plasmid kit#1000000229PurposeCollection of plasmids which extend the capabilities of the MoClo Yeast Toolkit (YTK) for genetically engineering S. cerevisiae.DepositorApplicationCloning and Synthetic BiologyVector typeYeast ExpressionCloning typeGolden Gate (MoClo)Available sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b-RvLEAMshort
Plasmid#218122PurposeExpresses truncated RvLEAM protein under IPTG induction in bacterial host cells.DepositorInsertRvLEAMshort
Tags6xHISExpressionBacterialMutationTruncated form (58-181aa)PromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLminP_Luc2P_RE10
Plasmid#90344PurposeIRF3 - Viral response gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterIRF3Available sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lamin A-mRFP
Plasmid#124268PurposeWild-type Lamin A, C-terminally tagged with mRFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNA (LMNA Human)
TagsmRFPExpressionMammalianAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQE9-His-p97(wt)
Plasmid#14666DepositorPublicationAvailable sinceApril 13, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTOX6
Plasmid#127451PurposeEncodes Tse2 toxin and amilCP chromoprotein. CAM-RDepositorInsertstse2 toxin
amilCP chromoprotein
ExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDY1243-Fluorescent RADARS
Plasmid#182540PurposeExpress Fluorescent RADARS in bicistronic vector with mCherry as control geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmNeon
ExpressionMammalianPromoterCMVAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-TAG-ERN
Plasmid#80476PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonExpressionMammalianAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by