We narrowed to 9,501 results for: pho;

1. pSG5-Myr-FLAG-p110α-DM

   Plasmid

   #55713

   Purpose

   eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   p110 alpha (Pik3ca Mouse)
   

   Tags

   myristoylation tag (Myr-FLAG)

   Expression

   Mammalian

   Mutation

   T207D, K227A

   Promoter

   T7

   Available Since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCIneoLuc-PP1A

   Plasmid

   #128348

   Purpose

   Vector for LUMIER assay

   Depositor

   Yutaka Hata

   Article

   Kawano et al J Biochem. 2015 May 14. pii: mvv051.

   Insert

   PP1A (PPP1CA Human)
   

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUAST-SIK3 T196A

   Plasmid

   #52974

   Purpose

   insect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)

   Depositor

   Biao Wang, Marc Montminy

   Article

   Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

   Insert

   SIK3 (Sik3 Fly)
   

   Tags

   HA

   Expression

   Insect

   Mutation

   T196A LKB1 phosphorylation defective mutant

   Promoter

   Hsp70

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pLenti-CMV-cGASΔN-HA (murine)

   Plasmid

   #130915

   Purpose

   Expresses murine cGASΔN (aa148-507)-HA; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   cGASΔN murine
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   murine cGAS catalytic domain (aa148-507)

   Promoter

   CMV

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-4T1-Claspin-MΔN

   Plasmid

   #31624

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

   Insert

   Claspin (CLSPN Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 899-1206 only

   Available Since

   Nov. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pGEX Nck-SH2

   Plasmid

   #46457

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Nck (NCK1 Human)
   

   Tags

   GST and Thrombin site

   Expression

   Bacterial

   Mutation

   contains amino acids 275-377

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX ShcA-SH2

   Plasmid

   #46486

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   ShcA (SHC1 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 476-584

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. GB1-PRD(800-868)-strep

   Plasmid

   #180023

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   GB1-His6-TEV-ALIX(800-868)-Strep (PDCD6IP Human)
   

   Tags

   GB1-His6-TEV and Strep tag (WSHPQFEK)

   Expression

   Bacterial

   Available Since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. shNg Lenti FHRC3J1UGW

   Plasmid

   #92233

   Purpose

   lentiviral expression of EGFP and Ng shRNA

   Depositor

   Weifeng Xu

   Article

   Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

   Insert

   shRNA targeting Ng
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PKM-IRES-hrGFP

    Plasmid

    #73405

    Purpose

    Mammalian expression of mouse PKM and hrGFP

    Depositor

    Raymond Swanson

    Article

    Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

    Insert

    PKM (Prkcz Mouse)
    

    Tags

    3xFLAG and pIRES-hrGFP-2a

    Expression

    Mammalian

    Mutation

    truncated, constitutively active form

    Promoter

    CMV

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pLJM60-S6K1(T389S) barcoded

    Plasmid

    #48800

    Purpose

    expresses mutant S6K1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    S6K1 T389S (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG and barcode: GGTACC

    Expression

    Mammalian

    Mutation

    T389S

    Promoter

    CMV

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. mCherry-C1 SNX9 KR mutant K267N R327N

    Plasmid

    #161994

    Purpose

    Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    SNX9 (Snx9 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    K267N R327N

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV asyn S87E

    Plasmid

    #36057

    Depositor

    Hilal Lashuel

    Article

    Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

    Insert

    alpha-synuclein S87E (SNCA Human)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S87E

    Promoter

    CMV

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pBS/U6/RNAi SadBs shRNA mouse

    Plasmid

    #67152

    Purpose

    pBS/U6 containing shRNA to mouse SADBs

    Depositor

    Ana Carrera, Maria Alvarado-Kristensson

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    BRSK1 (Brsk1 Mouse)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFBD-Akt1(T308A/S473A)

    Plasmid

    #86578

    Purpose

    Expresses human full-length Akt1(T308A/S473A) phosphomutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    T308A/S473A phosphomutant

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T Doc2beta C2AB(125) WT

    Plasmid

    #134690

    Purpose

    Encodes cytoplasmic domain of Doc2beta for bacterial expression and purification

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Double C2 protein beta (Doc2b Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRB113.2

    Plasmid

    #181948

    Purpose

    aTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    NarL-mNG; mCherry; tetR (AX04_RS23485, narL Synthetic, narL - E. coli)
    

    Tags

    mNeonGreen

    Expression

    Bacterial

    Promoter

    NarL-mNG-PLtetO-1; mCherry-PdcuS; tetR-J23106

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-UVRAG S498A

    Plasmid

    #86741

    Purpose

    N-terminal EGFP-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

    Insert

    human UVRAG (UVRAG Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S498A

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX SH2A-SH2

    Plasmid

    #46481

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SH2A (SH2D4A Human)
    

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 338-445

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-UVRAG S498A

    Plasmid

    #86744

    Purpose

    N-terminal mCherry-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

    Insert

    human UVRAG (UVRAG Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    S498A

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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