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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,177 results for: FRI;

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Showing: 441 - 480 of 3177 results

  4. PE-pegRNA(BG)-nsgRNA(BG)

    Plasmid
    #232727
    Purpose
    A dual-targeting vector that contains PE3 system guide RNAs against BFP (pegRNA(BG) and nsgRNA(BG)) with restriction enzyme sites for insertion of target site guide RNAS (pegRNAs(TS) and nsgRNA(TS)).
    Depositor
    David Brafman
    Article
    Frisch et al Mol Ther Nucleic Acids. 2023 Jul 17;33:4
    Insert
    pegRNA(BG), nsgRNA(BG), pegRNAs(TS), nsgRNA(TS)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232727-plasmid-map-sequence-id-462122

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  22. pETDuet-msGC-NT21

    Plasmid
    #78102
    Purpose
    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Depositor
    William Montfort
    Article
    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.
    Inserts
    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    fragment 1-380 of the beta subunit and fragment 2…
    Promoter
    T7
    Available Since
    June 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    78102-plasmid-map-sequence-id-151428

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  23. pETDuet-msGC-NT13

    Plasmid
    #75087
    Purpose
    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Depositor
    William Montfort
    Article
    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.
    Inserts
    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    fragment 1-380 of the beta subunit and fragment 4…
    Available Since
    May 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    75087-plasmid-map-sequence-id-148282

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  25. pEK42

    Plasmid
    #214958
    Purpose
    Endogenous tagging of B. dendrobatidis GAPDH with a red fluorescent protein, hygromycin B selection
    Depositor
    Lillian Fritz-Laylin
    Article
    Brody et al Proc Natl Acad Sci U S A. 2025 Nov 4;122
    Inserts
    BdGAPDH_LHA2-G4S-HsmRuby3-ScADH1ter
    SpH2Bpro-Hshph-BdGAPDH_RHA2
    Use
    Bd gene targeting vector
    Expression
    Bacterial
    Promoter
    SpH2Bpro
    Available Since
    Oct. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    214958-plasmid-map-sequence-id-426037

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  26. pEK48

    Plasmid
    #214964
    Purpose
    Endogenous tagging of B. dendrobatidis Myo17D with a red fluorescent protein, hygromycin B selection
    Depositor
    Lillian Fritz-Laylin
    Article
    Brody et al Proc Natl Acad Sci U S A. 2025 Nov 4;122
    Inserts
    BdMyo17D_LHA-G4S-HsmRuby3-ScADH1ter
    SpH2Bpro-Hshph-BdMyo17D_RHA
    Use
    Bd gene targeting vector
    Expression
    Bacterial
    Promoter
    SpH2Bpro
    Available Since
    Oct. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    214964-plasmid-map-sequence-id-426034

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  34. pMega-MaPylRS

    Plasmid
    #200225
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Tags
    none
    Expression
    Bacterial
    Mutation
    none
    Promoter
    proK-lacO and tacI
    Available Since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    200225-plasmid-map-sequence-id-400630

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  37. pMega-MaFRSA

    Plasmid
    #200226
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutated Asparagine 166 to Alanine and Valine 168 …
    Promoter
    proK-lacO and tacI
    Available Since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    200226-plasmid-map-sequence-id-393639

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  38. KCC2-pHext

    Plasmid
    #104076
    Purpose
    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)
    Depositor
    Igor Medyna
    Article
    Friedel et al eNeuro. 2017 Jul 21;4(4). pii: ENEURO.00
    Insert
    KCC2 (b isoform) (Slc12a5 Rat)
    Tags
    pHluorine tag inserted in the second putative tra…
    Expression
    Mammalian
    Mutation
    EcoR1 site in rat KCC2 was removed by silent muta…
    Promoter
    UbC
    Available Since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104076-plasmid-map-sequence-id-199360

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Showing: 441 - 480 of 3177 results