We narrowed to 4,889 results for: phy

1. pThom77.6

   Plasmid

   #101637

   Purpose

   Encodes the fluorescent protein amFP486/K68M under a CMV promoter.

   Depositor

   Thomas Hughes

   Article

   Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

   Insert

   amFP486/K68M
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K68M

   Promoter

   CMV

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pThom77.3

   Plasmid

   #101636

   Purpose

   Encodes the fluorescent protein amFP486/K68M for constitutive expression in E. coli.

   Depositor

   Thomas Hughes

   Article

   Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

   Insert

   amFP486/K68M
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   K68M

   Promoter

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-SARS-CoV-2 NSP13

   Plasmid

   #176492

   Purpose

   Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

   Depositor

   Xiaodong Cheng

   Article

   White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

   Insert

   SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)
   

   Use

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   I570 from SARS-CoV NSP13 was mutated to V to conv…

   Promoter

   T7

   Available since

   Jan. 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pET28a-RBP-GFP(S45C)-ZdK1

   Plasmid

   #185791

   Purpose

   Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker

   Depositor

   Lizhong He

   Article

   Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

   Insert

   RBP-sfGFP(S45C)-ZdK1
   

   Use

   Tags

   10xHis, TEV protease cleavage site after His Tag

   Expression

   Bacterial

   Mutation

   Ser to Cys mutation at start of GFP, residue 45 i…

   Promoter

   T7

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSM4

   Plasmid

   #101642

   Purpose

   Encodes the fluorescent protein KCyG4219 for constitutive expression in E. coli.

   Depositor

   Thomas Hughes

   Article

   Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

   Insert

   KCyG4219
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   C-terminal residues (221-223) deletion. Ser220Val…

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTK41

   Plasmid

   #236110

   Purpose

   Expresses the motor domain of rat KIF5C fused with SNAP tag and ALFA tag nanobody

   Depositor

   Shinsuke Niwa

   Article

   Kita et al Cell Rep Phys Sci, 6 (2): 102440. 19 Feb

   Inserts

   KIF5C(C7S)(1-430)-SNAP-His (Kif5c Rat)
   KIF5C(C7S)(1-430)-ALFA tag nanobody-StrepII tag (Kif5c Rat)
   

   Use

   Tags

   ALFA tag nanobody, His tag, SNAP tag, and StrepII…

   Expression

   Bacterial

   Mutation

   C7S mutation is introduced. 1-430 aa is encoded.

   Promoter

   T7

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDest17_hnRNPA1_FtoW

   Plasmid

   #224254

   Purpose

   Bacterial expression of N-terminally 6His-tagged hnRNPA1_FtoW

   Depositor

   Tanja Mittag

   Article

   Alshareedah et al Nat Phys. 2024 Sep;20(9):1482-1491. doi:

   Insert

   hnRNPA1_FtoW (HNRNPA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F17W, F25W, F31W, F37W, F43W, F62W, F69W, F78W, F…

   Promoter

   T7

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDest17_hnRNPA1_YtoW

   Plasmid

   #224253

   Purpose

   Bacterial expression of N-terminally 6His-tagged hnRNPA1_YtoW

   Depositor

   Tanja Mittag

   Article

   Alshareedah et al Nat Phys. 2024 Sep;20(9):1482-1491. doi:

   Insert

   hnRNPA1_YtoW (HNRNPA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y52W, Y59W, Y75W, Y81W, Y110W, Y120W, Y129W

   Promoter

   T7

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDest17_hnRNPA1_allW

   Plasmid

   #224252

   Purpose

   Bacterial expression of N-terminally 6His-tagged hnRNPA1_allW

   Depositor

   Tanja Mittag

   Article

   Alshareedah et al Nat Phys. 2024 Sep;20(9):1482-1491. doi:

   Insert

   hnRNPA1_allW (HNRNPA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F17W,F25W,F31W,F37W,F43W,Y52W, Y59W, F62W, F69W, …

   Promoter

   T7

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pACE-Truncated-Kir3.2-StreptagII

    Plasmid

    #177263

    Purpose

    Expresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cells

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al J Phys Chem Lett. 2021 Dec 30;12(51):122

    Insert

    G-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)
    

    Use

    Tags

    StreptagII

    Expression

    Insect

    Mutation

    Residues 49-378

    Promoter

    polyhedrin promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PCT48

    Pooled Library

    #170392

    Purpose

    Barcoded plasmid library

    Depositor

    Jonathan Weissman

    Article

    Jones et al Genome Biol. 2020 Apr 14;21(1):92. doi:

    Expression

    Mammalian

    Species

    Synthetic

    Use

    CRISPR and Lentiviral

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

12. Pan-Druggable Cancer Library

    Pooled Library

    #182133

    Purpose

    All-in-one CRISPR/Cas system with 74 individual elements. 63 elements target cancer-related genes with FDA-approved drug treatments, and 3 elements target key medulloblastoma driver genes.

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

13. pABD1275

    Plasmid

    #68017

    Purpose

    PIF1 protein overproduction for purification from bacterial cells

    Depositor

    Bruce Downie

    Article

    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11

    Insert

    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 AT2G20180, Mustard Weed)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Codon Optimized for bacterial expression (GenScri…

    Promoter

    T7

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pT2iApowriter2(#336)

    Plasmid

    #184104

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transient transgenesis

    Tags

    internal tag: 5myc

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT2iU6Apowriter2N(#355)

    Plasmid

    #184106

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transient transgenesis + in vitro trans…

    Tags

    internal tag: 5myc

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    Zf-Ubi+SP6

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT22Apowriter2(#340)

    Plasmid

    #184105

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    internal tag: 5myc

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT22iuasvApoWtEos3N(#486)

    Plasmid

    #184107

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    4xUAS

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT22iuasvApoWChe(#488)

    Plasmid

    #184108

    Purpose

    cyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    4xUAS

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pABD1031

    Plasmid

    #68004

    Purpose

    Protein co-localization of PIF1 (PIL5) via fluorescent fusion after transient expression in planta

    Depositor

    Bruce Downie

    Article

    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11

    Insert

    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 )
    

    Use

    Tags

    eYFP

    Expression

    Plant

    Mutation

    Promoter

    2X Cauliflower Mosaic Virus 35S promoter

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pAL101

    Plasmid

    #22474

    Purpose

    Expresses APB domain of PIF6 fused to mYFP, for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF6APB
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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