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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,982 results for: crispr grnas

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Showing: 2381 - 2400 of 13982 results

  3. pEh_gRNA1

    Plasmid
    #178758
    Purpose
    Expression of a gRNA that targets next to PAM library, used for E. haloalkaliphila type I-E system
    Depositor
    Chase Beisel
    Article
    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2
    Insert
    gRNA that targets next to PAM library, used for E. haloalkaliphila type I-E system
    Expression
    Bacterial
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178758-plasmid-map-sequence-id-355197

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  4. pMs_gRNA1

    Plasmid
    #178764
    Purpose
    Expression of a gRNA that targets next to PAM library, used for Marinomonas sp. type I-E system
    Depositor
    Chase Beisel
    Article
    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2
    Insert
    gRNA that targets next to PAM library, used for Marinomonas sp. type I-E system
    Expression
    Bacterial
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178764-plasmid-map-sequence-id-355195

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  5. pAc3_gRNA1

    Plasmid
    #178746
    Purpose
    Expression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #3 system
    Depositor
    Chase Beisel
    Article
    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2
    Insert
    gRNA that targets next to PAM library, used for A. chroococcum type I-E #3 system
    Expression
    Bacterial
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178746-plasmid-map-sequence-id-355198

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  7. pAc2_gRNA1

    Plasmid
    #178740
    Purpose
    Expression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #2 system
    Depositor
    Chase Beisel
    Article
    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2
    Insert
    gRNA that targets next to PAM library, used for A. chroococcum type I-E #2 system
    Expression
    Bacterial
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178740-plasmid-map-sequence-id-355194

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  11. Cas9-sgRNA-A+B

    Plasmid
    #149370
    Purpose
    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites
    Depositor
    Tarek Abbas
    Article
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Inserts
    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CBh, PGK, and U6
    Available Since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    149370-plasmid-map-sequence-id-315474

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  17. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

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  18. pCAG-memRFP-3xControlgRNA

    Plasmid
    #224567
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    mRFP1
    Use
    CRISPR
    Tags
    Membrane localization signal
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224567-plasmid-map-sequence-id-451479

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  19. pCAG-nucGFP11-3xControlgRNA

    Plasmid
    #224568
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    GFP11-H2B
    Use
    CRISPR
    Tags
    Histone H2B (nuclear localization)
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224568-plasmid-map-sequence-id-451496

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  20. pShoHELIX-sgRNA_entry (BO3)

    Plasmid
    #181784
    Purpose
    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181784-plasmid-map-sequence-id-358224

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Showing: 2381 - 2400 of 13982 results