We narrowed to 14,310 results for: eng
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Plasmid#1188DepositorAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pBLADE(FP6**)-mCherry
Plasmid#168049PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.DepositorInsertsBlue light-inducible AraC dimers in Escherichia coli
mCherry
ExpressionBacterialPromoterJ23101** and PBADAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pS448∙CsR
Plasmid#122096PurposeCRISPR/Cas9 counterselection in Gram-negative bacteriaDepositorInsertsCas9
sgRNA
UseSynthetic BiologyExpressionBacterialPromoterPem7 and PmAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEn-Chimera
Plasmid#61432PurposeGateway-Entry vector containing AtU6-26 promoter and sgRNA backboneDepositorInsertAtU6-26:sgRNA
UseCRISPRExpressionPlantPromoterAtU6-26Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJW1259
Plasmid#61251PurposeCas9 plasmid with sgRNA deleted; for use in C. elegansDepositorInsertDeletion
UseCRISPRExpressionWormMutationDeleted sgRNA(F+E) sequence from pJW1219Available sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cas9m2
Plasmid#47314PurposeCas9 D10A+H840ADepositorInsertCas9m2
UseCRISPRAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB WT
Plasmid#60419PurposeMammalian Expression of ITSN DH/PH-tgRFPt-NanoDepositorInserthITSN1-tgRFPt-SSPB (ITSN1 Human, Synthetic)
UseCre/Lox and LentiviralTagstgRFPtExpressionMammalianMutationhITSN 1159-1509 // SSPB Y11K and A15EPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD5-NS
Plasmid#149546PurposeExpression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOD_C2200
Plasmid#91082PurposeModule C, Promoter: none – to be combined with gRNA array in module B, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_B2203), Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRPromoternone (will be fused to gRNA array in MODULE B)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBI-MCS-EYFP
Plasmid#58855PurposeThe tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFPDepositorPublicationInsertenhanced yellow fluorescent protein
ExpressionMammalianPromoterCMVAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FUGW-APPSwe-T2A-mCherry
Plasmid#190804PurposeExpress Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherryDepositorAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
T4 td upstream intron-PABP
Plasmid#189972PurposePart 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAsDepositorInsertT4 td upstream intron-PABP
UseCloningPromoterN/AAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mouse SLC KO Library
Pooled library#141436PurposeKnockout library targeting mouse SLC proteins.DepositorExpressionMammalianSpeciesMus musculusUseCRISPR and LentiviralAvailable sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK_tBFP
Plasmid#85432PurposeLentiviral vector for Gal4 inducible human full-length TRAIL with mCherry reporter and constitutive tBFP expressionDepositorInserthuman TRAIL
UseLentiviralExpressionMammalianAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
J23104-mRFP1-331Bb
Plasmid#78274PurposemRFP1 behind constitutive promoter J23104 in pSEVA331Bb backboneDepositorInsertJ23104-mRFP1
Available sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCEV-G2-Km
Plasmid#46815PurposeTEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneTagsFLAG and c-mycExpressionYeastPromoterTEF1 and PGK1Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTD-NStrepHis
Plasmid#45936Purposeuse to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tagDepositorTypeEmpty backboneTags6xHis and Strep IIExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX458-Dual-Guide-Donor-ShuttleOnly
Plasmid#171098PurposeDonor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.DepositorTypeEmpty backboneUseCRISPRMutationXbaI site added upstream of U6 promotor. GtoC mu…Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-NLS-AcrIIA4
Plasmid#113037PurposeExpression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)DepositorInsertSV40 NLS-AcrIIA4
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by